LANCE® TR-FRET Epigenetics Tool Box

Consistent, validated solutions for your most demanding epigenetics applications.

Proven, cost-efficient LANCE Ultra reagents can be used to quantitate peptide ififications, detecting specific meythylation and acetylation states. No wash, homogenous LANCE Ultra reagents are HTS friendly - design your own epigenetics screening strategy for greatest efficiency.

LANCE Ultra time-resolved fluorescence resonance energy transfer (TR-FRET) assays use a proprietary europium chelate donor dye, together with ULight™, an innovative small molecular weight acceptor dye with a red-shifted fluorescent emission.

Epigenetic enzymatic assays are optimized using a biotinylated histone H3-derived peptide as substrate. The modified peptide is captured by the Eu-labeled antibody (Ab) and ULight-Streptavidin (SA) which bring the Eu donor and ULight acceptor dye molecules into close proximity. Upon irradiation at 320 or 340 nm, the energy from the Eu donor is transferred to the ULight acceptor dye which, in turn, generates light at 665 nm. The intensity of the light emission is proportional to the level of biotinylated substrate modification.

LANCE Ultra
Acetyl Modifications
ReagentsValidation:  Technical Data Sheets
TargetMark SpecificityAcetyltransferasesDeacetylases
Histone H3Unmodified (H3K4)HDAC2
SIRT1
Unmodified H3K9/K27HDAC1
H3K9acp300
H3K27acHDAC1
Histone H4H4Kac panTIP60
p53K382acSIRT1
TauO-GlcNAcOGA
Methyl Modifications
ReagentsValidation:  Technical Data Sheets
TargetMark SpecificityMethyltransferasesDemethylases
Histone H3Unmodified (H3K4)LSD1
Unmodified H3K9/K27JMJD1A
H3K4me1-2SET7/9 (peptide)
H3K9me2G9aJMJD2C
H3K27me2-1EZH2JMJD3
H3K27me3 
H3K36meJMJD2A
H3/H4Rme panPRMT4
Histone H4H4R3mePRMT1

Products

LANCE® Ultra Europium-anti-acetyl-Histone H3 Lysine 9 (H3K9ac)

Europium-labeled monoclonal antibody recognizing human Histone H3 acetylated at lysine 9 (H3K9ac). ...  View more

LANCE® Ultra Europium-anti-methyl-Histone H3 Lysine 4 (H3K4me1-2)

Europium-labeled monoclonal antibody recognizing human Histone H3 mono- or di-methylated at lysine ...  View more

LANCE® Ultra Europium-anti-methyl-Histone H3 Lysine 9 (H3K9me2)

Europium-labeled monoclonal antibody recognizing human Histone H3 di-methylated at lysine 9 (H3K9me2)....  View more

LANCE® Ultra Europium-anti-unmodified-Histone H3 Lysine 9/Lysine 27 (H3K9/K27) Antibody

Europium-labeled monoclonal antibody recognizing human Histone H3 that is unmodified at lysine 9 and...  View more

LANCE® Ultra Europium-anti-acetyl-Histone H3 Lysine 27 (H3K27ac) Antibody

Europium-labeled monoclonal antibody recognizing human Histone H3 acetylated at lysine 27 (H3K27ac)....  View more

LANCE® Ultra Europium-anti-acetyl-Histone H4 Lysine (H4Kac pan) Antibody

Europium-labeled monoclonal antibody recognizing human Histone H4 acetylated lysine residues (H4Kac ...  View more

LANCE® Ultra Europium-anti-acetyl-p53 Lysine 382 (p53K382ac) Antibody

Europium-labeled monoclonal antibody recognizing human p53 acetylated at lysine 382 (p53K382ac). Broad...  View more

LANCE® Ultra Europium-anti-di/mono-methyl-Histone H3 Lysine 27 (H3K27me2-1) Antibody

Europium-labeled monoclonal antibody recognizing human Histone H3 mono- or di-methylated at lysine 27...  View more

LANCE® Ultra Europium-anti-di-methyl-Histone H3 Lysine 36 (H3K36me2) Antibody

Europium-labeled monoclonal antibody recognizing human Histone H3 di-methylated at lysine 36 (H3K36me2)....  View more

LANCE® Ultra Europium-anti-methyl-Histone H3/H4 Arginine (H3/H4Rme pan) Antibody

Europium-labeled monoclonal antibody recognizing human Histone H3 and H4 methylated arginine residues...  View more

LANCE® Ultra Europium-anti-methyl-Histone H4 Arginine 3 (H4R3me) Antibody

Europium-labeled monoclonal antibody recognizing human Histone H4 methylated at arginine 3 (H4R3me)....  View more

LANCE® Ultra Europium-anti-O-linked-GlcNAc Antibody

Europium-labeled monoclonal antibody recognizing O-linked n-acetylglucosamine (O-linked GlcNAc). Broad...  View more

LANCE® Ultra Europium-anti-tri-methyl-Histone H3 Lysine 27 (H3K27me3) Antibody

Europium-labeled monoclonal antibody recognizing human Histone H3 tri-methylated at lysine 27 (H3K27me3)....  View more

LANCE® Ultra Europium-anti-unmodified-Histone H3 Lysine 4 (H3K4) Antibody

Europium-labeled monoclonal antibody recognizing human Histone H3 that is unmodified at lysine 4 (H3K4)....  View more