PerkinElmer

Alpha Reagents

OF

Alpha Technology

Virtually anything can be measured with Alpha Technology quickly and easily, including enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein:protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous, no-wash assay, with no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerbrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the Alpha solution that’s right for you

 

  • AlphaLISA – when running large numbers of ELISA from the same sample or working with difficult matrix, serum or plasma
  • AlphaLISA SureFire Ultra and AlphaScreen SureFire – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaScreen – perfect for studying fusion-tagged proteins or protein:protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf kit, the toolbox gives you everything you need to create your own

AlphaLISA assay principle
AlphaLISA assay principle

Biomarker/cytokine detection

Detection and quantitation of molecular targets in serum, plasma, culture supernatants, cellular lysates, and other sample types.Convert troublesome traditional ELISAs to a far superior alternative - PerkinElmer's highly sensitive, homogeneous AlphaLISA platform for biomarkers and cytokines. Our exclusive AlphaLISA assay platform offers a wide dynamic range and easy automation for analysis of biomarkers, cytokines and other analytes. With AlphaLISA you can also shorten the time it takes you to run assays to less than 3 hours and benefit from small sample volumes (1-5 µL) to conserve precious sample.
Alpha_cell_signal.jpg
Alpha_cell_signal.jpg

Cell signaling

AlphaScreen SureFire, AlphaLISA SureFire Ultra, and Alpha SureFire Multiplex assay kits to measure protein phosphorylation in cell signaling pathways.

  • All-in-one-well alternative to Western Blotting
  • Flexibility to use cultured cell lines, stem cells and primary cells
  • Cost-effective and flexible – a single plate can accommodate any assay for any target
  • Great sensitivity and dynamic range
  • Easy automation
  • No harmful chemicals and non-radioactive, eliminating special reagent handling and disposal
Alpha_PPI.jpg
Alpha_PPI.jpg

Protein:protein interactions

Handle large biomolecular interactions with ease.

  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders
  • Conserve reagents: robust assays using nM concentrations of proteins/antibodies

 

AL717_AL721_AL723_assay_principle
AL717_AL721_AL723_assay_principle

Epigenetics

Highly sensitive no wash detection for measuring biochemical modification of peptides, proteins, histones, and nucleosomes. Cellular Detection Kits for rapid and direct detection of endogenous modification of epigenetic marks. Kits and reagents for writer, reader, and eraser assays.

  • Flexibility: Work with peptide or full-length protein substrates, or endogenous histones in cell-based format
  • Sensitivity: Robust and reproducible detection, even at low enzyme concentrations
  • Specificity: Reagents highly specific for the mark of interest
  • Simplicity: One-well, no-wash assay platforms amenable to automation and HTS
  • Validated: Detailed, ready-to-use protocols for over 30 epigenetic enzyme assays

 

Alpha_cAMP.jpg
Alpha_cAMP.jpg

GPCR research

Measure cAMP and downstream phosphorylation of proteins for functional GPCR assays.
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"Alpha Reagents" 1-50 of 855 Products and Services

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AlphaLISA immunoassay kits

Residual Protein A AlphaLISA Detection Kit

The AlphaLISA Residual Protein A Detection Kit is designed for the quantitative detection of Protein A contaminants in buffered solution using a homogeneous (no wash steps, no separation steps) assay.
Price Range : 476.00 GBP - 3970.00 GBP
AlphaLISA immunoassay kits

IgG2 (human) isotyping AlphaLISA Detection Ki

The AlphaLISA Human IgG2 Detection Kit is designed for detection, quantitation, and isotyping of human IgG2 in cell culture medium and non-human serum, using a homogeneous (no wash steps, no separation steps) assay. The assay shows negligible cross-reactivity with other human IgG isotypes and monkey IgG.
Price Range : 374.00 GBP - 5830.00 GBP
AlphaLisa Analyte 2

PIGF (human), lyophilized (AlphaLISA)

Human placental growth factor (PIGF) standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human PIGF Detection Kit, but can also be ordered separately.
List Price : 146.00 GBP
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-eIF4E (Ser209) Assay Kits

The AlphaLISA® SureFire® Ultra™ p-eIF4E(Ser209) assay is a sandwich immunoassay for quantitative detection of phospho-eIF4E (phosphorylated on Ser209) in cellular lysates using Alpha Technology.
Price Range : 307.00 GBP - 17790.00 GBP
AlphaLISA immunoassay kits

IL-1ß (rat) AlphaLISA Detection Kit

The AlphaLISA® Rat Interleukin 1ß (IL-1ß) Detection Kit is designed for detection and quantitation of rat IL-1ß in bronchial lavage fluid (BALF), buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 954.00 GBP - 6330.00 GBP

Protein G AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to Protein G. These beads can be used to capture antibodies that associate with Protein G.
Price Range : 369.00 GBP - 15140.00 GBP
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-ERK1/2 (Thr202/Tyr204) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-ERK 1/2 (Thr202/Tyr204) assay is a sandwich immunoassay for quantitative detection of phospho-ERK 1/2 (phosphorylated on Thr202/Tyr204 in ERK1, or Thr185/Tyr187 in ERK2) in cellular lysates using Alpha Technology.
Price Range : 307.00 GBP - 17790.00 GBP

Anti-Human IgG1 AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to a anti-human IgG1. This bead can be used to create no-wash AlphaLISA assays for isotyping and other applications.
Price Range : 354.00 GBP - 14540.00 GBP
AlphaLisa Analyte 2

C-Peptide (rat), lyophilized (AlphaLISA)

Rat Connecting Peptide (C-Peptide) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Mouse/Rat C-Peptide kit, but can be ordered separately.
List Price : 332.00 GBP
AlphaLISA immunoassay kits

Factor VIII (human) AlphaLISA Biotin-Free Detection Kit

The AlphaLISA® Human BDNF Biotin-Free Detection Kit is designed for the quantitative detection of human Brain Derived Neurotrophic Factor (BDNF) in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit is compatible with high-biotin culture media and other sample types that contain high levels biotin.
Price Range : 288.00 GBP - 6300.00 GBP

Protein A AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to Protein A. These beads can be used to capture antibodies that associate with Protein A.
Price Range : 369.00 GBP - 15140.00 GBP
AlphaLISA immunoassay kits

C-Reactive Protein (human) AlphaLISA Detection Kit

The AlphaLISA® Human C-Reactive Protein (CRP) Detection Kit is designed for detection and quantitation of human CRP in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 954.00 GBP - 6330.00 GBP
AlphaLISA immunoassay kits

IL-18 (bovine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for bovine IL-18 enables the quantitative determination of bovine interleukin 18 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 271.00 GBP - 5940.00 GBP

Anti-Human IgG3 AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to a anti-human IgG3. This bead can be used to create no-wash AlphaLISA assays for isotyping and other applications.
Price Range : 354.00 GBP - 14540.00 GBP
Alpha donor antibody coated

Anti-rat IgG Alpha Donor Beads

Alpha Donor beads conjugated to anti-rat IgG antibody. This bead can be used to capture rat IgG antibodies for Alpha no-wash protein detection, antibody detection, antibody binding, and other applications.
Price Range : 301.00 GBP - 5460.00 GBP
AlphaLISA immunoassay kits

Fibrinogen (human) AlphaLISA Detection Kit

The AlphaLISA® Human Fibrinogen Detection Kit is designed for detection and quantitation of Human fibrinogen in human serum, plasma, and cell culture supernatants using a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 280.00 GBP - 6300.00 GBP

Utrophin (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human utrophin enables the quantitative determination of utrophin in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 181.00 GBP - 11290.00 GBP
AlphaLISA immunoassay kits

Lambda light chain (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human lambda light chain enables the quantitative determination of human λ light chain in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 376.00 GBP - 9030.00 GBP
AlphaLISA immunoassay kits

Cry1F AlphaLISA Detection Kit

The AlphaLISA® Cry1F Detection Kit is designed for detection and quantitation of Cry1F in GMO seeds, leaf, and plant extracts using a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 376.00 GBP - 5840.00 GBP

SCF (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human SCF enables the quantitative determination of stem cell factor (SCF; KIT-ligand) in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 271.00 GBP - 5940.00 GBP

IL-12/IL-23 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human IL-12 enables the detection and quantitation of human Interleukin 12 p40 (IL12 p40) in human serum or cell culture medium using a homogeneous AlphaLISA assay (no wash steps). It is important to note that this assay will also detect IL-23 p40.
Price Range : 437.00 GBP - 12290.00 GBP
alphaimmunoassay1

IL-10 (human) AlphaLISA Detection Kit

The AlphaLISA® Human Interleukin-10 (IL-10) Detection Kit is designed for detection and quantitation of human IL-10 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 954.00 GBP - 6330.00 GBP

AlphaScreen DIG (Digoxin/Digoxigenin) Detection Kit

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Anti-Dig (digoxin) conjugated AlphaScreen Acceptor beads. 
Price Range : 113.00 GBP - 8640.00 GBP
AlphaLisa Analyte 2

VEGF (human), lyophilized (AlphaLISA)

Human VEGF, lyophilized, 0.3 µg. The VEGF analyte corresponds to VEGF165 (isoform d). VEGF is included in the AlphaLISA® human VEGF kits, but can also be ordered separately as a standalone item. One vial contains an amount of human VEGF sufficient for performing 10 standard curves.
List Price : 155.00 GBP

AlphaScreen PhosphoSensor Kit

Antibody-free assay for kinase activity via dectection of phosphorylated protein or peptide substrate.
Price Range : 226.00 GBP - 1810.00 GBP
AlphaLisa Analyte 2

IL-1β (mouse), lyophilized (AlphaLISA)

Mouse Interleukin-1ß (IL-1ß) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Mouse IL-1ß kit, but can be ordered separately.
List Price : 155.00 GBP

GAD67 (Human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for GAD67 enables the quantitative determination of human glutamate decarboxylase 67 (hGAD67) in serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows less than 5% cross reactivity with mouse GAD67. Cross-reactivity with other species has not tested.
Price Range : 485.00 GBP - 15510.00 GBP
AlphaLISA Buffer

AlphaLISA universal buffer

Pre-formulated buffer used to QC Alpha assays. This buffer can sometimes be used for protein-protein binding, proof-of-concept, and other Alpha assays.
Price Range : 127.00 GBP - 241.00 GBP

Nickel Chelate AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to nickel chelate. These beads can be used to capture His-tagged proteins and peptides.
Price Range : 369.00 GBP - 15140.00 GBP

Anti-Maltose-Binding Protein (MBP) AlphaLISA® Acceptor beads

AlphaLISA® Acceptor beads conjugated to anti-MBP (maltose-binding protein) antibody. The antibody is a rat monoclonal antibody, clone MBP 7G4. These beads can be used to capture MBP-tagged proteins for AlphaLISA no-wash assays.
Price Range : 369.00 GBP - 15140.00 GBP
AlphaPlex Samarium Acceptor Beads

Anti-Mouse IgG AlphaPlex™ 645 (Sm) Acceptor Beads

AlphaPlex™-645 (Samarium) Acceptor beads conjugated to anti-mouse IgG antibody. These beads are used to capture mouse IgG antibodies (targeting the Fc region).
Price Range : 369.00 GBP - 15130.00 GBP
AlphaLISA immunoassay kits

PSMA (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human PSMA enables the quantitative determination of human prostate specific membrane antigen (PSMA) in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 376.00 GBP - 9030.00 GBP
AlphaLISA Cellular Epigenetics kits

AlphaLISA Acetylated-Histone H3 Lysine 27 (H3K27ac) Cellular Detection Kit

This kit is designed for the detection of acetylated Histone H3 Lysine 27 (H3K27ac) in cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 415.00 GBP - 7400.00 GBP
AlphaLISA immunoassay kits

Heme oxygenase 2 AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human heme oxygenase 2 (HO-2) enables the quantitative determination of human HO-2 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 271.00 GBP - 5940.00 GBP
AlphaLISA Buffer

AlphaLISA HiBlock Buffer

Pre-formulated buffer that can be used as a high blocking buffer for Alpha analyte detection assays. This buffer is included in select AlphaLISA immunoassay kits, but can also be purchased to develop your own Alpha assay.
Price Range : 127.00 GBP - 241.00 GBP
AlphaLISA immunoassay kits

PIGF (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human placental growth factor (PIGF) enables the quantitative determination of human PIGF (PGF) in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 271.00 GBP - 5940.00 GBP
AlphaLisa Analyte 2

IGF2 (human), lyophilized (AlphaLISA)

Human Insulin-like Growth Factor 2 (IGF2) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human IGF2 kit, but can be ordered separately.
List Price : 155.00 GBP

IL-10 (mouse) AlphaLISA Detection Kit

The AlphaLISA® Mouse Interleukin-10 (IL-10) Detection Kit is designed for detection and quantitation of mouse IL-10 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 954.00 GBP - 6330.00 GBP

PTEN (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human PTEN enables the detection and quantitation of human PTEN in tissue homogenates and cell lysates using a homogeneous AlphaLISA assay (no wash steps). Other species of PTEN were not tested, but detection is expected with mouse and rat PTEN due to >99% sequence homology.
Price Range : 288.00 GBP - 6300.00 GBP
Alphaplex-545-terbium-glutathione

Glutathione AlphaPlex™ 545 Acceptor Beads

AlphaPlex™-545 (Terbium) Acceptor beads conjugated to glutathione, for use in Alpha no-wash multiplexing assays.
Price Range : 369.00 GBP - 15130.00 GBP

EGFR (human) AlphaLISA Detection Kit

The AlphaLISA® EGFR Detection Kit is designed for detection and quantitation of human Epidermal Growth Factor Receptor (EGFR) in cell culture media, human serum, and other sample types using a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 436.00 GBP - 6310.00 GBP

AlphaLISA Anti-acetyl-Histone H3 Lysine 27 (H3K27ac) Acceptor Beads

AlphaLISA® Acceptor beads conjugated to an antibody against human histone H3 acetylated at lysine 27 (H3K27ac). These beads can be used for no-wash AlphaLISA epigenetic writer and eraser assays.
Price Range : 849.00 GBP - 15450.00 GBP
AlphaLisa Analyte 2

ANGPTL4 (human), lyophilized (AlphaLISA)

Human ANGPTL4 standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human ANGPTL4 Detection Kit, but can also be ordered separately.
List Price : 146.00 GBP
AlphaPlex Samarium immunoassay kits

AlphaPlex-645 (Sm) Human IP10 Detection Kit

The AlphaPlex Samarium (Sm) Human Interferon Gamma-induced Protein 10 (hIP10) Detection Kit is designed for the quantitative determination of human IP10 in serum, plasma, and cell culture supernatants using a homogeneous (no wash steps, no separation steps) assay.
Price Range : 432.00 GBP - 6300.00 GBP
SureFire principle

AlphaScreen SureFire Phospho-MEK 1 Kit

The AlphaScreen® SureFire® p-MEK1 (Ser218/222) assay is a sandwich immunoassay for quantitative detection of phospho-MEK1 (phosphorylated on Ser218/222) in cellular lysates using Alpha Technology.
Price Range : 936.00 GBP - 10110.00 GBP
AlphaPlex Samarium immunoassay kits

AlphaPlex-645 (Sm) Human IL17 Detection Kit

The AlphaPlex™ 645 (Samarium) Human IL17 Detection Kit is designed for the quantitative determination of human IL17 together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) multiplexing assay.
Price Range : 225.00 GBP - 6300.00 GBP

AlphaScreen HA (Hemagglutinin) Detection Kit

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Anti-HA (hemagglutinin) conjugated AlphaScreen Acceptor beads. 
Price Range : 113.00 GBP - 8640.00 GBP
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra AKT 1/2/3 (pS473) Assay Kit

The AlphaLISA® SureFire® Ultra p-AKT1/2/3 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-AKT1/2/3 (phosphorylated on Ser473) in cellular lysates using Alpha Technology.
Price Range : 307.00 GBP - 17790.00 GBP
AlphaLISA immunoassay kits

Nidogen-1 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human Nidogen-1 enables the detection and quantitation of Human Nidogen-1 (NID-1; also known as Entactin) in human serum, plasma, and, cell lysyate, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 280.00 GBP - 6110.00 GBP

ANGPT1 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human ANGPT1 enables the quantitative determination of human Angiopoietin 1 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 376.00 GBP - 11290.00 GBP

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