PerkinElmer

Alpha

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Alpha Technology

Virtually anything can be measured with Alpha Technology quickly and easily, including enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein:protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous, no-wash assay, with no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerbrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the Alpha solution that’s right for you:

  • AlphaLISA – when running large numbers of ELISA from the same sample or working with difficult matrix, serum or plasma
  • AlphaLISA SureFire Ultra and AlphaScreen SureFire – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaScreen – perfect for studying fusion-tagged proteins or protein:protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf kit, the toolbox gives you everything you need to create your own

AlphaLISA assay principle
AlphaLISA assay principle

Biomarker/cytokine detection

Detection and quantitation of molecular targets in serum, plasma, culture supernatants, cellular lysates, and other sample types.Convert troublesome traditional ELISAs to a far superior alternative - PerkinElmer's highly sensitive, homogeneous AlphaLISA platform for biomarkers and cytokines. Our exclusive AlphaLISA assay platform offers a wide dynamic range and easy automation for analysis of biomarkers, cytokines and other analytes. With AlphaLISA you can also shorten the time it takes you to run assays to less than 3 hours and benefit from small sample volumes (1-5 µL) to conserve precious sample.
Alpha_cell_signal.jpg
Alpha_cell_signal.jpg

Cell signaling

AlphaScreen SureFire, AlphaLISA SureFire Ultra, and Alpha SureFire Multiplex assay kits to measure protein phosphorylation in cell signaling pathways.

  • All-in-one-well alternative to Western Blotting
  • Flexibility to use cultured cell lines, stem cells and primary cells
  • Cost-effective and flexible – a single plate can accommodate any assay for any target
  • Great sensitivity and dynamic range
  • Easy automation
  • No harmful chemicals and non-radioactive, eliminating special reagent handling and disposal
Alpha_PPI.jpg
Alpha_PPI.jpg

Protein:protein interactions

Handle large biomolecular interactions with ease.

  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders
  • Conserve reagents: robust assays using nM concentrations of proteins/antibodies

 

AL717_AL721_AL723_assay_principle
AL717_AL721_AL723_assay_principle

Epigenetics

 

Epigenetics is the analysis of heritable traits that are not transmitted by sequence information. For methylation analysis, NEXTflex® Bisulfite-Seq and Methyl-Seq Kits are available to simplify whole genome bisulfite sequencing, reduced representation bisulfite sequencing, and MeDIP or MeCap sequencing. To analyze how proteins interact with DNA, NEXTflex® ChIP-Seq Library Preparation Kits, requiring very low input amounts, simplify ChIP-seq experiments and reliably produce libraries from difficult samples.

Alpha_cAMP.jpg
Alpha_cAMP.jpg

GPCR research

Measure cAMP and downstream phosphorylation of proteins for functional GPCR assays.
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"Alpha Reagents" 1-50 of 915 Products and Services

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AlphaLisa Analyte 2

Amyloid β 1-40 (rodent), lyophilized (AlphaLISA)

Mouse/Rat Amyloid beta (Aß 1-40) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Mouse/Rat Aß 1-40 kit, but can be ordered separately.
List Price : 332.00 GBP
SureFire principle

AlphaScreen SureFire Phospho-AKT (Thr308) Kit

The AlphaScreen® SureFire® p-Akt1 (Thr308) assay is a sandwich immunoassay for quantitative detection of phospho-Akt1 (phosphorylated on Thr308) in cellular lysates using Alpha Technology.
Price Range : 936.00 GBP - 10110.00 GBP
AlphaLisa Analyte 2

Soybean Agglutinin, liquid (AlphaLISA)

Soybean Agglutinin standard, liquid, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Soybean Agglutinin Detection Kit, but can be ordered separately.
List Price : 155.00 GBP
AlphaPlex Samarium Acceptor Beads

Protein A AlphaPlex™ 645 (Sm) Acceptor Beads

AlphaPlex-645 (Samarium) Acceptor beads conjugated to Protein A. These beads are used to capture immunoglobulins through their heavy chain (Fc region) allowing optimal orientation and antigen detection. Protein A has higher affinity for rabbit, pig, dog and cat IgG.
Price Range : 369.00 GBP - 15130.00 GBP
AlphaLISA immunoassay kits

CXCL10/IP-10 (mouse) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for mouse Interferon Gamma Induced Protein 10 (mIP-10)/CXCL10 enables the quantitative determination of mouse IP-10 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 271.00 GBP - 5940.00 GBP

Anti-rat IgG (Fc specific) AlphaLISA® Acceptor Beads

AlphaLISA® Acceptor beads conjugated to anti-rat IgG antibody. These beads can be used to capture rat IgG antibodies. The antibody is Fc-specific, and is a goat polyclonal F(ab')2 fragment.
Price Range : 369.00 GBP - 15140.00 GBP
AlphaLISA immunoassay kits

CXCL4/PF4 (Human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for CXCL4/PF4 enables the quantitative determination of CXCL4/PF4 in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows minumum cross-reactivity with mouse CXCL4/PF4 and human CXCL4L1/PF4V.
Price Range : 388.00 GBP - 11630.00 GBP
AlphaLISA immunoassay kits

ApoC3 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human ApoC3 enables the quantitative determination of apolipoprotein C3 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 376.00 GBP - 11290.00 GBP
AlphaLISA Buffer

5X AlphaLISA PPI Buffer

General protein-protein interaction assay buffer for AlphaLISA no-wash binding assays. The PPI buffer (5X) has been specially developed for AlphaLISA protein-protein interaction assays.
Price Range : 252.00 GBP - 569.00 GBP
AlphaLISA immunoassay kits

IL-17A (bovine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for bovine IL-17A enables the detection and quantitation of bovine Interleukin 17A (IL17A) in bovine serum or cell culture medium using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 437.00 GBP - 12290.00 GBP
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra Total GAPDH (Human/Mouse) Assay Kit

The AlphaLISA® SureFire® Ultra Mouse/Human GAPDH assay is a sandwich immunoassay for quantitative detection of human or mouse GAPDH in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization assay.
Price Range : 298.00 GBP - 17300.00 GBP
AlphaPlex Samarium Acceptor Beads

Unconjugated AlphaPlex™ 545 Acceptor Beads

Unconjugated AlphaPlex 645 (Samarium) Acceptor beads bear reactive aldehyde groups at their surface, allowing you to conjugate a wide variety of reagents using a simple reductive amination protocol.
Price Range : 842.00 GBP - 17740.00 GBP
AlphaLisa Analyte 2

GAD67 (Human) lyophilized (AlphaLISA)

Human GAD67 standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA GAD67 Detection Kit, but can be ordered separately.
List Price : 186.00 GBP
AlphaLISA immunoassay kits

IL-8 (human) AlphaLISA Biotin-Free Detection Kit

The AlphaLISA Human IL8 Biotin-Free Detection Kit is designed for the quantitative detection of human IL-8 in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).
Price Range : 436.00 GBP - 6310.00 GBP
AlphaLISA Cellular Epigenetics kits

AlphaLISA Human Total Histone H4 Cell-based Assay Kit

AlphaLISA no-wash assay kit for the detection of total human Histone H4 in cell extracts or cells in culture medium in a lysate transfer assay.
Price Range : 514.00 GBP - 10060.00 GBP
AlphaLISA Buffer

AlphaLISA NaCl Buffer

Pre-formulated buffer that can be used to dissociate Protein A from IgG in bioprocess samples. This buffer is included in select AlphaLISA immunoassay kits, but can also be purchased to develop your own Alpha assay.
Price Range : 123.00 GBP - 234.00 GBP
AlphaLISA immunoassay kits

IL-6 (monkey) AlphaLISA Detection Kit, 100 Assay Points

The AlphaLISA® immunoassay kit for monkey IL-6 enables the quantitative determination of cynomolgus monkey interleukin-6 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 376.00 GBP - 11290.00 GBP
AlphaLISA immunoassay kits

FLT-1/VEGFR 1 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human VEGFR 1 (FLT-1) enables the quantitative determination of VEGFR 1 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 271.00 GBP - 5940.00 GBP
AlphaLisa Analyte 2

IL-1β (mouse), lyophilized (AlphaLISA)

Mouse Interleukin-1ß (IL-1ß) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Mouse IL-1ß kit, but can be ordered separately.
List Price : 155.00 GBP
AlphaLisa Analyte 2

Kappa light chain (human), lyophilized (AlphaLISA)

Human kappa light chain standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human Kappa Light Chain Detection Kit, but can also be ordered separately.
List Price : 146.00 GBP
AlphaLISA immunoassay kits

ApoE (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for ApoE enables the quantitative determination of Apolipopriein E (ApoE) in human serum, plasma, cell lysate, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows minimum cross-reactivity with ApoA1, ApoB, and ApoCIII. Crossreactivity with other species has not tested.
Price Range : 388.00 GBP - 11630.00 GBP
PerkinElmer

SF UltraPlexTb CaptSure Acc Beads

CaptSure-coated AlphaPlex-545 (Terbium) Acceptor Beads, for multiplexing SureFire assays. These beads are already included in our Alpha SureFire Ultra Multiplex kits, but can be ordered separately as needed.
Price Range : 166.00 GBP - 6860.00 GBP
AlphaLISA immunoassay kits

IL-33 (mouse) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for mouse IL-33 enables the quantitative determination of mouse interleukin-33 in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 376.00 GBP - 9030.00 GBP
AlphaPlex Terbium immunoassay kits

AlphaPlex-545 (Tb) Human ICAM-1 Detection Kit

The AlphaPlex™-545 (Tb) Human ICAM-1 Detection Kit is designed for detection and quantitation of human ICAM-1 in cell culture supernatant and human serum using a homogeneous AlphaPlex multiplexing assays (no wash steps).
Price Range : 405.00 GBP - 14280.00 GBP
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra Total Akt1 Assay Kit

The AlphaLISA® SureFire® Ultra™ Total Akt1 assay is a sandwich immunoassay for quantitative detection of Akt1 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
Price Range : 307.00 GBP - 17790.00 GBP

Anti-Mouse IgG2a (isotyping) AlphaLISA Acceptor beads

AlphaLISA® Acceptor beads conjugated to a anti-mouse IgG2a. This bead can be used to create no-wash AlphaLISA assays for isotyping and other applications.
Price Range : 354.00 GBP - 14540.00 GBP
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-STAT6 (Tyr641) Assay Kit

The AlphaLISA® SureFire® Ultra p-STAT6 (Tyr641) assay is a sandwich immunoassay for quantitative detection of phospho-STAT6 (phosphorylated on Tyr641) in cellular lysates using Alpha Technology.
Price Range : 298.00 GBP - 17300.00 GBP
AlphaPlex Terbium immunoassay kits

AlphaPlex-545 (Tb) Human TNFα Detection Kit

The AlphaPlex™ 545 (Terbium) Human TNFα Detection Kit is designed for the quantitative determination of human TNFα together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) multiplexing assay.
Price Range : 381.00 GBP - 6300.00 GBP
AlphaLISA immunoassay kits

ANGPTL4 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human ANGPTL4 enables the quantitative determination of ANGPTL4 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 271.00 GBP - 5940.00 GBP
AlphaLISA immunoassay kits

Somatostatin (human) AlphaLISA Detection Kit

The AlphaLISA® Somatostatin Detection Kit is designed for detection and quantitation of somatostatin in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 436.00 GBP - 6300.00 GBP
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-SLP-76 (Ser376) Assay Kit, 50,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-SLP-76 (Ser376) assay is a sandwich immunoassay for quantitative detection of phospho-SLP-76 (phosphorylated on Ser376) in cellular lysates using Alpha no-wash technology.
List Price : 17820.00 GBP

Anti-acetyl-Histone H3 Lysine 9 (H3K9ac) AlphaLISA® Acceptor Beads

AlphaLISA® Acceptor beads conjugated to an antibody against human histone H3 acetylated at lysine 9 (H3K9Ac). These beads can be used for no-wash AlphaLISA epigenetic writer and eraser assays.
Price Range : 883.00 GBP - 16070.00 GBP

AlphaLISA Biotinylated Anti-Histone H3 (C-ter) Antibody

Biotinylated antibody against the C-terminal end of human histone H3, for use in Alpha no-wash epigenetic assays using full-length histone H3 as substrate.
Price Range : 662.00 GBP - 2340.00 GBP
AlphaLISA immunoassay kits

IL-4 (bovine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for bovine IL-4 enables the detection and quantitation of bovine interleukin-4 in serum, buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 280.00 GBP - 6110.00 GBP
Protein

Cardiac Troponin T (human), lyophilized (AlphaLISA)

Human cardiac troponin T standard, for use in running standard curves in AlphaLISA assays.
List Price : 191.00 GBP
AlphaLISA immunoassay kits

Cortisol (human) AlphaLISA Detection Kit

AlphaLISA no-wash immunoassay kit for detection of human cortisol in buffer or serum.
Price Range : 436.00 GBP - 6310.00 GBP
AlphaLISA immunoassay kits

IL-25 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human IL-25 enables the quantitative determination of human interleukin 25 (IL-25) in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 376.00 GBP - 9030.00 GBP
AlphaLISA immunoassay kits

D-dimer (human) AlphaLISA Detection Kit

The AlphaLISA® Human D-dimer Detection Kit is designed for detection and quantitation of human D-dimer in citrate plasma, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. Please note the kit shows some cross-reactivity to D-monomer.
Price Range : 1830.00 GBP - 14280.00 GBP
SureFire principle

AlphaScreen SureFire 4EBP1 (p-Thr37/Thr46) Assay Kit

The AlphaScreen® SureFire® p-4EBP1 (Thr37/46) assay is a sandwich immunoassay for quantitative detection of phospho-4EBP1 (phosphorylated on Thr37/46) in cellular lysates using Alpha Technology.
Price Range : 1030.00 GBP - 11100.00 GBP
Alpha SureFire Ultra Multiplex kits

Alpha SureFire Ultra Multiplex p-AKT (Ser473) (directly coupled) Kit

Single target-only kit for multiplexing to other AlphaLISA SureFire Ultra assay kits. The Alpha SureFire® Ultra Multiplex kits allow the rapid, sensitive, and quantitative detection of phosphoproteins from cells, combined with the measurement of another protein, phospho or total. This Alpha Multiplex measurement is carried out in the same assay plate well from a single sample of cell lysate, and is achieved by the use of two types of Alpha Acceptor beads that emit at distinct wavelengths (545nm and 615nm).
Price Range : 292.00 GBP - 16980.00 GBP
AlphaLISA immunoassay kits

ApoA1 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for ApoA1 is designed for the quantitative determination of ApoA1 in human serum, human plasma and cell supernantants using a homogeneous AlphaLISA assay (no wash steps). Neglible cross reactivity was observed with ApoCIII, ApoE, or ApoB.
Price Range : 388.00 GBP - 11630.00 GBP
AlphaLisa Analyte 2

CXCL10/IP-10 (mouse), lyophilized (AlphaLISA)

Mouse IP-10 (CXCL10) standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA mouse IP-10 Detection Kit, but can also be ordered separately.
List Price : 146.00 GBP

Anti-C-tag AlphaLISA Acceptor Beads

AlphaLISA Acceptor beads conjugated to anti-C-tag antibody. These beads can be used to capture C-tag proteins, and can be used in conjunction with Alpha Donor beads to create AlphaLISA no-wash assays.
Price Range : 369.00 GBP - 15130.00 GBP

AlphaLISA Anti-acetyl-Histone H4 Lysine (H4Kac pan) Acceptor Beads

AlphaLISA® Acceptor beads conjugated to an antibody against acetylated lysine residues on human Histone H4 (H4Kac pan). These beads can be used for AlphaLISA no-wash epigenetic writer and eraser assays.
Price Range : 849.00 GBP - 15450.00 GBP
SureFire principle

AlphaScreen SureFire Phospho-BAD (Ser136) Kit

The AlphaScreen® SureFire® p-BAD (Ser136) assay is a sandwich immunoassay for quantitative detection of phospho-BAD (phosphorylated on Ser136) in cellular lysates using Alpha Technology.
Price Range : 936.00 GBP - 10110.00 GBP
AlphaLISA immunoassay kits

IGF-1 (rat) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for rat IGF-1 enables the quantitative determination of rat insulin-like growth factor 1 (IGF-1) in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 376.00 GBP - 11290.00 GBP
AlphaLISA immunoassay kits

CD80 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human CD80 enables the quantitative determination of human cluster of differentiation 80 (CD80) in serum, buffered solution, and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 470.00 GBP - 9030.00 GBP
AlphaLisa Analyte 2

α-tubulin standard, liquid (AlphaLISA)

Human α-tubulin standard, liquid, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA α-tubulin Detection Kit, but can be ordered separately.
List Price : 267.00 GBP

AlphaLISA Anti-unmodified Histone H3 Lysine 9/Lysine 27 (H3K9/K27) Acceptor Beads

AlphaLISA® Acceptor beads conjugated to an antibody against human Histone H3 that is unmodified at lysine 9 and lysine 27. These beads can be used for AlphaLISA no-wash epigenetic eraser assays.
Price Range : 849.00 GBP - 15450.00 GBP

AlphaScreen Rabbit IgG Detection Kit

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Anti-rabbit IgG conjugated AlphaScreen Acceptor beads. This kit contains enough reagents to run 500 wells in 384-well format, using a 25 µL reaction volume.
Price Range : 113.00 GBP - 8640.00 GBP

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