PerkinElmer

Alpha Reagents

OF

Virtually any target can be quickly and easily measured using Alpha technology, an improved alternative to quantitative ELISA assays and ELISA reagents. Such targets include enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein-protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous assay with no wash requirement and no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerebrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the homogenous assay right for you:

  • AlphaLISA® – when running large numbers of ELISA assays from the same sample or working with a difficult matrix, serum or plasma
  • AlphaLISA® SureFire® Ultra and AlphaScreen® SureFire® – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaScreen® – perfect for studying fusion-tagged proteins or protein-protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf quantitative ELISA assay, the toolbox gives you everything you need to create your own

AlphaLISA assay principle
AlphaLISA assay principle

Biomarker/cytokine detection

Detection and quantitation of molecular targets in serum, plasma, culture supernatants, cellular lysates, and other sample types.Convert troublesome traditional ELISAs to a far superior alternative - PerkinElmer's highly sensitive, homogeneous AlphaLISA platform for biomarkers and cytokines. Our exclusive AlphaLISA assay platform offers a wide dynamic range and easy automation for analysis of biomarkers, cytokines and other analytes. With AlphaLISA you can also shorten the time it takes you to run assays to less than 3 hours and benefit from small sample volumes (1-5 µL) to conserve precious sample.
Alpha_cell_signal.jpg
Alpha_cell_signal.jpg

Cell signaling

AlphaScreen SureFire, AlphaLISA SureFire Ultra, and Alpha SureFire Multiplex assay kits to measure protein phosphorylation in cell signaling pathways.

  • All-in-one-well alternative to Western Blotting
  • Flexibility to use cultured cell lines, stem cells and primary cells
  • Cost-effective and flexible – a single plate can accommodate any assay for any target
  • Great sensitivity and dynamic range
  • Easy automation
  • No harmful chemicals and non-radioactive, eliminating special reagent handling and disposal
Alpha_PPI.jpg
Alpha_PPI.jpg

Protein:protein interactions

Handle large biomolecular interactions with ease.

  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders
  • Conserve reagents: robust assays using nM concentrations of proteins/antibodies

 

AL717_AL721_AL723_assay_principle
AL717_AL721_AL723_assay_principle

Epigenetics

 

Epigenetics is the analysis of heritable traits that are not transmitted by sequence information. For methylation analysis, NEXTflex® Bisulfite-Seq and Methyl-Seq Kits are available to simplify whole genome bisulfite sequencing, reduced representation bisulfite sequencing, and MeDIP or MeCap sequencing. To analyze how proteins interact with DNA, NEXTflex® ChIP-Seq Library Preparation Kits, requiring very low input amounts, simplify ChIP-seq experiments and reliably produce libraries from difficult samples.

Alpha_cAMP.jpg
Alpha_cAMP.jpg

GPCR research

Measure cAMP and downstream phosphorylation of proteins for functional GPCR assays.
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"Alpha Reagents" 1-50 of 913 Products and Services

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AlphaScreen Phosphotyrosine (PY20) Assay Kit

AlphaScreen phosphoprotein kits are designed to detection phosphoproteins in biochemical or cellular kinase assays. This kit contains streptavidin Donor beads and AlphaScreen Acceptor beads conjugated with the antiphosphotyrosine PY20 antibody. This kit can be used to capture biotinylated phosphoproteins, biotinylated phosphorylated peptides, or biotinylated antibodies in complex with phosphorylated proteins.
Price Range : 116.00 GBP - 8900.00 GBP
AlphaLISA SureFire Ultra kits

Phospho-Ribosomal Protein S6 (Ser240/244) AlphaLISA SureFire Ultra Assay Kit

The AlphaLISA® SureFire® Ultra™ p-rpS6 (Ser240/244) assay is a sandwich immunoassay for quantitative detection of phospho-Ribosomal Protein S6 (phosphorylated on Ser240/244) in cellular lysates using Alpha no-wash technology.
Price Range : 316.00 GBP - 18330.00 GBP
SureFire principle

AlphaScreen SureFire SMAD2 (p-Ser465;Ser467) Assay Kit

The AlphaScreen® SureFire® p-SMAD2 (p-Ser465/467) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD2 (phosphorylated on Ser465/467) in cellular lysates using Alpha Technology.
Price Range : 965.00 GBP - 10430.00 GBP
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-eIF2α (Ser51) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-eIF2α (Ser51) assay is a sandwich immunoassay for quantitative detection of phospho-eIF2α (phosphorylated on Ser51) in cellular lysates using Alpha Technology.
Price Range : 316.00 GBP - 18330.00 GBP
SureFire principle

AlphaScreen SureFire Phospho-Topo IIa (Thr1343) Assay Kit

The AlphaScreen® SureFire® p-Topoisomerase II alpha (p-Thr1343) assay is a sandwich immunoassay for quantitative detection of phospho-Topoisomerase II alpha (phosphorylated on Thr1343) in cellular lysates using Alpha Technology.
Price Range : 965.00 GBP - 10430.00 GBP
AlphaLISA immunoassay kits

CXCL9/MIG (human) AlphaLISA Detection Kit

The AlphaLISA® Human C-X-C Motif Chemokine 9 / Monokine Induced by Gamma Interferon (CXCL9 / MIG) Detection Kit is designed for detection and quantitation of human CXCL9/MIG in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1880.00 GBP - 14710.00 GBP
AlphaLisa Analyte 2

IL-33 (mouse), lyophilized (AlphaLISA)

Mouse IL-33 standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA mouse IL-33 Detection Kit, but can also be ordered separately.
List Price : 191.00 GBP
SureFire principle

AlphaScreen SureFire Phospho-Topo IIa (Ser1106) Assay Kit

The AlphaScreen® SureFire® p-Topoisomerase II alpha (p-Ser1106) assay is a sandwich immunoassay for quantitative detection of phospho-Topoisomerase II alpha (phosphorylated on Ser1106) in cellular lysates using Alpha Technology.
Price Range : 965.00 GBP - 10430.00 GBP

AlphaScreen DIG (Digoxin/Digoxigenin) Detection Kit

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Anti-Dig (digoxin) conjugated AlphaScreen Acceptor beads. 
Price Range : 116.00 GBP - 8900.00 GBP
AlphaLISA assay principle

ANGPT2 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human ANGPT2 enables the quantitative determination of human Angiopoietin 2 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 388.00 GBP - 11630.00 GBP
SureFire principle

AlphaScreen SureFire ALK (p-Tyr1604) Assay Kit

The AlphaScreen® SureFire® p-ALK (Tyr1604) assay is a sandwich immunoassay for quantitative detection of phospho-ALK (phosphorylated on Tyr1604) in cellular lysates using Alpha Technology.
Price Range : 965.00 GBP - 10430.00 GBP
AlphaLISA immunoassay kits

IL-4 (mouse) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for mouse IL-4 enables the quantitative determination of mouse interleukin-4 in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 388.00 GBP - 9300.00 GBP
AlphaLISA immunoassay kits

CD117 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human CD117 enables the quantitative determination of human CD117 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 280.00 GBP - 6120.00 GBP
alphascreen surefire total

AlphaScreen SureFire Total IkB alpha Assay Kit

The AlphaScreen® SureFire® total p70 S6K assay is a sandwich immunoassay for quantitative detection of p70 S6K (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
Price Range : 965.00 GBP - 10430.00 GBP
alphascreen surefire total

AlphaScreen SureFire p53 Total Assay Kit

The AlphaScreen® SureFire® total p53 assay is a sandwich immunoassay for quantitative detection of p53 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
Price Range : 264.00 GBP - 10430.00 GBP
AlphaLISA immunoassay kits

IL-33 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human IL-33 enables the quantitative determination of human interleukin-33 in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 388.00 GBP - 9300.00 GBP
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-VEGF Receptor 2 (Tyr1175) Assay Kit

The AlphaLISA® SureFire® Ultrap-VEGF Receptor 2 (Tyr1175) assay is a sandwich immunoassay for quantitative detection of phospho-VEGFR Receptor 2 (phosphorylated on Tyr1175) in cellular lysates using Alpha Technology.
Price Range : 316.00 GBP - 18330.00 GBP
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-SYK (Tyr525/526) Assay Kit

The AlphaLISA® SureFire® Ultra p-SYK (Tyr525/526) assay is a sandwich immunoassay for quantitative detection of phospho-SYK (phosphorylated on Tyr525/526) in cellular lysates using Alpha Technology.
Price Range : 316.00 GBP - 18330.00 GBP
Protein

ANGPT2 (human), lyophilized (AlphaLISA)

Human Angiopoietin 2 (ANGPT2) standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human ANGPT2 Detection Kit, but can also be ordered separately.
List Price : 191.00 GBP
AlphaLISA immunoassay kits

IL-6 (bovine) AlphaLISA Detection Kit

AlphaLISA no-wash immunoassay kit for quantitative detection of bovine IL-6. This kit permits detection of bovine IL-6 in bovine serum, plasma, and cell culture supernatants.
Price Range : 450.00 GBP - 6660.00 GBP
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-CREB (Ser133) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-CREB (Ser133) in a multiplexed assay.
Price Range : 226.00 GBP - 13610.00 GBP

Anti-C-tag AlphaLISA Acceptor Beads

AlphaLISA Acceptor beads conjugated to anti-C-tag antibody. These beads can be used to capture C-tag proteins, and can be used in conjunction with Alpha Donor beads to create AlphaLISA no-wash assays.
Price Range : 380.00 GBP - 15590.00 GBP
AlphaLISA immunoassay kits

TNFα (bovine) AlphaLISA Detection Kit

The AlphaLISA immunoassay kit for detection and quantitation of bovine TNFα in serum, plasma, cell culture supernatants, and other sample types allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
Price Range : 417.00 GBP - 6660.00 GBP
SureFire principle

AlphaScreen SureFire ATF1 (p-Ser63) Assay Kit

The AlphaScreen® SureFire® p-ATF1 (Ser63) assay is a sandwich immunoassay for quantitative detection of phospho-ATF1 (phosphorylated on Ser63) in cellular lysates using Alpha Technology.
Price Range : 965.00 GBP - 10430.00 GBP
AlphaLISA immunoassay kits

Host Cell Residual DNA AlphaLISA Detection Kit

The AlphaLISA® Host Cell Residual DNA Detection Kit is designed for detection and quantitation of DNA contaminants in cell culture supernatants using a homogeneous (no-wash steps, no separation steps) assay. This kit can be used with DNA from different prokaryotic and eukaryotic species.
Price Range : 449.00 GBP - 6490.00 GBP
AlphaLisa Analyte 2

IL-13 (mouse), lyophilized (AlphaLISA)

Mouse IL-13 standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA mouse IL-13 Detection Kit, but can also be ordered separately.
List Price : 191.00 GBP

Anti-DIG AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to a mouse monoclonal anti-Dig (digoxin) antibody. These beads can be used to capture Dig-labeled proteins, peptides, and other biomolecules to create AlphaLISA no-wash assays.
Price Range : 380.00 GBP - 15590.00 GBP
Alpha SureFire Ultra Multiplex kits

Alpha SureFire® Ultra™ HV Multiplex p-Akt 1/2/3 (S473) (Eu) + Total Akt1 (Tb) Kit

The Alpha SureFire® Ultra™ HV Multiplex p-Akt 1/2/3 (Ser473) + Total Akt 1 assay kit is used to measure both the phosphorylation (Ser473) and total levels of endogenous Akt in cellular lysates. The kit measures phosphorylation on Ser473 of Akt 1/2/3, and total levels of Akt 1 only. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure Akt (Ser473) activation by either recombinant or endogenous receptors, and can be applied to primary cells.
Price Range : 542.00 GBP - 31470.00 GBP
alphascreen surefire total

AlphaScreen SureFire Total Akt1 research kit

The AlphaScreen® SureFire® Akt 1 (total) assay is a sandwich immunoassay for quantitative detection of Akt 1 in cellular lysates using Alpha Technology. The assay is an ideal system for normalizing Akt levels for experiments measuring changes in Akt phosphorylation.
Price Range : 1070.00 GBP - 11430.00 GBP
AlphaLISA immunoassay kits

IL-6 (monkey) AlphaLISA Detection Kit, 100 Assay Points

The AlphaLISA® immunoassay kit for monkey IL-6 enables the quantitative determination of cynomolgus monkey interleukin-6 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 388.00 GBP - 11630.00 GBP
AlphaLISA immunoassay kits

C-Peptide (mouse/rat) AlphaLISA Detection Kit

The AlphaLISA® Mouse/Rat Connecting Peptide (C-Peptide) Detection Kit is designed for detection and quantitation of mouse or rat C-Peptide in buffered solution in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1880.00 GBP - 14710.00 GBP
alphascreen surefire total

AlphaScreen SureFire Total p38 MAPK Assay Kit

The AlphaScreen® SureFire® total p38 MAPK assay is a sandwich immunoassay for quantitative detection of p38 MAPK (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
Price Range : 1070.00 GBP - 11430.00 GBP
AlphaPlex Terbium immunoassay kits

α-tubulin (human) AlphaPlex Terbium Detection Kit

The AlphaPlex™ Terbium (Tb) Human α-Tubulin Detection Kit is designed for the quantitative determination of human α-tubulin in serum, plasma, and cell culture supernatants using a homogeneous (no wash steps, no separation steps) assay. This assay can be used as a cell normalization or protein level normalization assay, in conjunction with AlphaLISA® biomarker detection kits.
Price Range : 470.00 GBP - 9030.00 GBP

AlphaLISA Anti-tri-methyl-Histone H3 Lysine 27 (H3K27me3) Acceptor Beads

AlphaLISA® Acceptor beads conjugated to an antibody against human histone H3 tri-methylated at lysine 27 (H3K27me3). These beads can be used for no-wash AlphaLISA epigenetic writer and eraser assays.
Price Range : 874.00 GBP - 15910.00 GBP
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-mTOR (Ser2448) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-mTOR (Ser2448) in a multiplexed assay.
Price Range : 226.00 GBP - 13610.00 GBP
AlphaLISA immunoassay kits

Progesterone AlphaLISA Detection Kit

The AlphaLISA® Progesterone Detection Kit is designed for detection and quantitation of progesterone in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 449.00 GBP - 6500.00 GBP
AlphaLISA immunoassay kits

Neprilysin (human) AlphaLISA Detection Kit

The AlphaLISA® Neprilysin Detection Kit is designed for detection and quantitation of human neprilysin (NEP, CD10) in cell culture media, serum, and CSF (cerebrospinal fluid) in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 449.00 GBP - 6500.00 GBP
AlphaLISA immunoassay kits

BRCA2 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human breast cancer type 2 susceptibility protein (BRCA2) enables the quantitative determination of human BRCA2 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 280.00 GBP - 6120.00 GBP
AlphaLISA immunoassay kits

IL-17A (serum) (mouse) AlphaLISA Detection Kit

AlphaLISA no-wash immunoassay kit for detection and quantitation of mouse IL-17 (IL-17A homodimer) in serum, cell culture supernatants, and other samples.
Price Range : 404.00 GBP - 6500.00 GBP
Alpha donor antibody coated

Anti-DIG Alpha Donor beads

Alpha Donor beads conjugated to anti-DIG (digoxin) antibody. This bead can be used to capture Dig-labeled biomolecules for Alpha no-wash assays.
Price Range : 332.00 GBP - 5650.00 GBP
AlphaLISA immunoassay kits

IFNγ (cyno) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for monkey cynomolgus interferon gamma (IFNγ) enables the quantitative determination of cynomolgus IFNγ in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 280.00 GBP - 6120.00 GBP

Anti-goat IgG (Fc specific) AlphaLISA® Acceptor Beads

AlphaLISA® Acceptor beads conjugated to anti-goat IgG antibody. These beads can be used to capture goat IgG antibodies. The antibody is Fc-specific, and is a rabbit polyclonal F(ab')2 fragment.
Price Range : 380.00 GBP - 15590.00 GBP
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-4E-BP1 (Thr37/46) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-4E-BP1 (Thr37/46) in a multiplexed assay.
Price Range : 226.00 GBP - 13610.00 GBP
SureFire principle

AlphaScreen SureFire Phospho-ATF2 (Thr71) Assay Kit

The AlphaScreen® SureFire® p-ATF2 (Thr71) assay is a sandwich immunoassay for quantitative detection of phospho-ATF2 (phosphorylated on Thr71) in cellular lysates using Alpha Technology.
Price Range : 965.00 GBP - 10430.00 GBP
AlphaLisa Analyte 2

Claudin 7 (human), lyophilized (AlphaLISA)

Human claudin 7 standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Human Claudin 7 Detection Kit, but can be ordered separately.
List Price : 275.00 GBP
AlphaLISA Cellular Epigenetics kits

AlphaLISA dimethylated-Histone H3 Lysine 36 (H3K36me2) Cellular Detection Kit

This kit is designed for the detection of di-methylated Histone H3 Lysine 36 (H3K36me2) in cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 428.00 GBP - 7620.00 GBP
AlphaLISA immunoassay kits

TNFα (cynomolgus) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for cynomolgus TNFα enables the quantitative determination of cynomolgus tumor necrosis factor alpha in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 280.00 GBP - 6120.00 GBP

Strep-Tactin® AlphaLISA® Acceptor beads

AlphaLISA® Acceptor beads conjugated to Strep-Tactin®. These beads can be used to capture Strep-tag® II-tagged proteins for AlphaLISA no-wash assays.
Price Range : 369.00 GBP - 15150.00 GBP
AlphaLISA immunoassay kits

TSLP (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human TSLP enables the quantitative determination of human TSLP in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 388.00 GBP - 9300.00 GBP
SureFire principle

AlphaScreen SureFire p70 S6K (p-Thr389) Assay Kit

The AlphaScreen® SureFire® p-p70 S6K (Thr389) assay is a sandwich immunoassay for quantitative detection of phospho-p70 S6K (phosphorylated on Thr389) in cellular lysates using Alpha Technology.
Price Range : 1070.00 GBP - 11430.00 GBP

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