PerkinElmer

Alpha

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Alpha Technology

Virtually anything can be measured with Alpha Technology quickly and easily, including enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein:protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous, no-wash assay, with no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerbrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the Alpha solution that’s right for you

 

  • AlphaLISA – when running large numbers of ELISA from the same sample or working with difficult matrix, serum or plasma
  • AlphaLISA SureFire Ultra and AlphaScreen SureFire – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaScreen – perfect for studying fusion-tagged proteins or protein:protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf kit, the toolbox gives you everything you need to create your own

AlphaLISA assay principle
AlphaLISA assay principle

Biomarker/cytokine detection

Detection and quantitation of molecular targets in serum, plasma, culture supernatants, cellular lysates, and other sample types.Convert troublesome traditional ELISAs to a far superior alternative - PerkinElmer's highly sensitive, homogeneous AlphaLISA platform for biomarkers and cytokines. Our exclusive AlphaLISA assay platform offers a wide dynamic range and easy automation for analysis of biomarkers, cytokines and other analytes. With AlphaLISA you can also shorten the time it takes you to run assays to less than 3 hours and benefit from small sample volumes (1-5 µL) to conserve precious sample.
Alpha_cell_signal.jpg
Alpha_cell_signal.jpg

Cell signaling

AlphaScreen SureFire, AlphaLISA SureFire Ultra, and Alpha SureFire Multiplex assay kits to measure protein phosphorylation in cell signaling pathways.

  • All-in-one-well alternative to Western Blotting
  • Flexibility to use cultured cell lines, stem cells and primary cells
  • Cost-effective and flexible – a single plate can accommodate any assay for any target
  • Great sensitivity and dynamic range
  • Easy automation
  • No harmful chemicals and non-radioactive, eliminating special reagent handling and disposal
Alpha_PPI.jpg
Alpha_PPI.jpg

Protein:protein interactions

Handle large biomolecular interactions with ease.

  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders
  • Conserve reagents: robust assays using nM concentrations of proteins/antibodies

 

AL717_AL721_AL723_assay_principle
AL717_AL721_AL723_assay_principle

Epigenetics

Highly sensitive no wash detection for measuring biochemical modification of peptides, proteins, histones, and nucleosomes. Cellular Detection Kits for rapid and direct detection of endogenous modification of epigenetic marks. Kits and reagents for writer, reader, and eraser assays.

  • Flexibility: Work with peptide or full-length protein substrates, or endogenous histones in cell-based format
  • Sensitivity: Robust and reproducible detection, even at low enzyme concentrations
  • Specificity: Reagents highly specific for the mark of interest
  • Simplicity: One-well, no-wash assay platforms amenable to automation and HTS
  • Validated: Detailed, ready-to-use protocols for over 30 epigenetic enzyme assays

 

Alpha_cAMP.jpg
Alpha_cAMP.jpg

GPCR research

Measure cAMP and downstream phosphorylation of proteins for functional GPCR assays.
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"Alpha Reagents" 1-50 of 887 Products and Services

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AlphaLISA immunoassay kits

Collagen IV (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human collagen IV enables the quantitative determination of collagen IV in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 271.00 GBP - 5940.00 GBP
AlphaLISA immunoassay kits

Corticosterone (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human corticosterone enables the quantitative determination of corticosterone in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 271.00 GBP - 5940.00 GBP

AlphaScreen GST Detection Kit

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Anti-GST conjugated AlphaScreen Acceptor beads. This kit contains enough reagents to run 500 wells in 384-well format, using a 25 µL reaction volume.
Price Range : 113.00 GBP - 8640.00 GBP
AlphaLisa Analyte 2

Collagen IV (human), lyophilized (AlphaLISA)

Human collagen IV standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA Human Collagen IV Detection Kit, but can also be ordered separately.
List Price : 146.00 GBP

AlphaScreen FITC (Fluorescein) Detection Kit

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Anti-FITC conjugated AlphaScreen Acceptor beads. 
Price Range : 113.00 GBP - 8640.00 GBP
AlphaLISA immunoassay kits

CXCL4/PF4 (Human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for CXCL4/PF4 enables the quantitative determination of CXCL4/PF4 in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows minumum cross-reactivity with mouse CXCL4/PF4 and human CXCL4L1/PF4V.
Price Range : 388.00 GBP - 11630.00 GBP
AlphaLisa Analyte 2

Corticosterone (human), liquid (AlphaLISA)

Human corticosterone standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human Corticosterone Detection Kit, but can also be ordered separately.
List Price : 146.00 GBP
AlphaLISA immunoassay kits

CTLA-4 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) enables the quantitative determination of human CTLA-4 in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 376.00 GBP - 9030.00 GBP
AlphaLISA immunoassay kits

CCL3/MIP-1α (human) AlphaLISA Detection Kit

The AlphaLISA® Human C-C Motif Chemokine 3 / Macrophage Inflammatory Protein-1alpha (CCL3 / MIP-1α) Detection Kit is designed for detection and quantitation of human CCL3/MIP-1α in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 954.00 GBP - 6330.00 GBP

AlphaScreen General IgG (Protein A) Detection Kit

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Protein A conjugated AlphaScreen Acceptor beads.
Price Range : 56.00 GBP - 8640.00 GBP
AlphaLisa Analyte 2

PSA (human), lyophilized (AlphaLISA)

Human Prostate-Specific Antigen (PSA) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human PSA kit, but can be ordered separately.
List Price : 155.00 GBP
AlphaLISA immunoassay kits

IL-13 (mouse) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for mouse IL-13 enables the quantitative determination of mouse interleukin-13 in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 376.00 GBP - 9030.00 GBP

AlphaScreen Rabbit IgG Detection Kit

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Anti-rabbit IgG conjugated AlphaScreen Acceptor beads. This kit contains enough reagents to run 500 wells in 384-well format, using a 25 µL reaction volume.
Price Range : 113.00 GBP - 8640.00 GBP
SureFire principle

AlphaScreen SureFire mTOR (p-Ser2448) Assay Kit

The AlphaScreen® SureFire® p-mTOR (Ser2448) assay is a sandwich immunoassay for quantitative detection of phospho-mTOR (phosphorylated on Ser2448) in cellular lysates using Alpha Technology.
Price Range : 936.00 GBP - 10110.00 GBP

AlphaScreen Phosphotyrosine (PT66) Assay Kit

AlphaScreen phosphoprotein kits are designed to detection phosphoproteins in biochemical or cellular kinase assays. This kit contains streptavidin Donor beads and AlphaScreen Acceptor beads conjugated with the antiphosphotyrosine PT66 antibody. This kit can be used to capture biotinylated phosphoproteins, biotinylated phosphorylated peptides, or biotinylated antibodies in complex with phosphorylated proteins.
Price Range : 113.00 GBP - 8640.00 GBP
AlphaLISA immunoassay kits

CXCL9/MIG (human) AlphaLISA Detection Kit

The AlphaLISA® Human C-X-C Motif Chemokine 9 / Monokine Induced by Gamma Interferon (CXCL9 / MIG) Detection Kit is designed for detection and quantitation of human CXCL9/MIG in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1830.00 GBP - 14280.00 GBP

Anti-FITC AlphaLISA® Acceptor beads

AlphaLISA® Acceptor beads conjugated to mouse monoclonal anti-FITC antibody. These beads can be used to capture FITC-tagged and fluorescein-labeled biomolecules for AlphaLISA no-wash assays.
Price Range : 369.00 GBP - 15140.00 GBP
AlphaLISA immunoassay kits

AlphaLISA Human Programmed Cell Death Protein 1 (PD-1) Detection Kit

The AlphaLISA® Human PD-1 Detection Kit is designed for detection and quantitation of Human Programmed Cell Death Protein 1 (PD-1) in human serum, plasma, and cell culture supernatants using a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 405.00 GBP - 6310.00 GBP
AlphaLISA immunoassay kits

IgG4 (human) isotyping AlphaLISA Detection Kit

The AlphaLISA Human IgG4 Detection Kit is designed for detection, quantitation, and isotyping of human IgG4 in cell culture medium and non-human serum, using a homogeneous (no wash steps, no separation steps) assay. The assay shows negligible cross-reactivity with other human IgG isotypes and monkey IgG.
Price Range : 389.00 GBP - 6060.00 GBP
PerkinElmer

AlphaLISA Cell-Histone Buffer Set

Pre-formulated buffer for cell-based Alpha epigenetic assays.
Price Range : 509.00 GBP - 4010.00 GBP
AlphaLISA immunoassay kits

C-Reactive Protein (human) AlphaLISA Detection Kit

The AlphaLISA® Human C-Reactive Protein (CRP) Detection Kit is designed for detection and quantitation of human CRP in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 954.00 GBP - 6330.00 GBP
SureFire principle

AlphaScreen SureFire MKK4 (p-Ser257;Thr261) Assay Kit

The AlphaScreen® SureFire® p-MKK4 (Ser257/Thr261) assay is a sandwich immunoassay for quantitative detection of phospho-MKK4 (phosphorylated on Ser257/Thr261) in cellular lysates using Alpha Technology.
Price Range : 936.00 GBP - 10110.00 GBP
PerkinElmer

FCGR3A/CD16A (176Phe/F158) (human) AlphaLISA Detection Kit

This kit is designed for the detection of binding activity between human FCGR3A (176Phe) and human IgG Fc fragment using a homogeneous AlphaLISA® assay (no wash steps). This assay can facilitate the design and development of antibody therapetics by using competitive binding.
Price Range : 366.00 GBP - 6660.00 GBP
alphascreen surefire total

AlphaScreen SureFire Total Topo Iia Assay Kit

The AlphaScreen® SureFire® total Topoisomerase II assay is a sandwich immunoassay for quantitative detection of Topoisomerase II (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
Price Range : 936.00 GBP - 10120.00 GBP
alphaimmunoassay1

IL-2 (human) AlphaLISA Detection Kit

The AlphaLISA® Human Interleukin-2 (IL2) Detection Kit is designed for detection and quantitation of human IL-2 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 954.00 GBP - 6330.00 GBP
AlphaLISA immunoassay kits

IgM (human) AlphaLISA Detection Kit

The AlphaLISA® Human IgM Protein Detection Kit is designed for detection and quantitation of human IgM in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.
Price Range : 476.00 GBP - 3970.00 GBP
AlphaLisa Analyte 2

CD86 (human), lyophilized (AlphaLISA)

Human CD86 standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human CD86 Detection Kit, but can also be ordered separately.
List Price : 185.00 GBP
Assay principle for LANCE TR-FRET binding assay

CTLA4-CD86 AlphaLISA Binding Kit

This kit is designed for the detection of binding activity between Human CTLA-4 and CD86, using a homogeneous AlphaLISA assay (no wash steps). This assay can facilitate the design and development of antibody therapetics by using competitive binding to CTLA-4/CD86.
Price Range : 1130.00 GBP - 6880.00 GBP
Alpha donor antibody coated

Anti-DIG Alpha Donor beads

Alpha Donor beads conjugated to anti-DIG (digoxin) antibody. This bead can be used to capture Dig-labeled biomolecules for Alpha no-wash assays.
Price Range : 302.00 GBP - 5480.00 GBP

Anti-Mouse IgG2a (isotyping) AlphaLISA Acceptor beads

AlphaLISA® Acceptor beads conjugated to a anti-mouse IgG2a. This bead can be used to create no-wash AlphaLISA assays for isotyping and other applications.
Price Range : 354.00 GBP - 14540.00 GBP
SureFire principle

AlphaScreen SureFire JNK 1-3 (p-Thr183;Tyr185) Assay Kit

The AlphaScreen® SureFire® p-JNK1/3 (Thr183/Tyr185) assay is a sandwich immunoassay for quantitative detection of phospho-JNK1/3 (phosphorylated on Thr183/Tyr185) in cellular lysates using Alpha Technology.
Price Range : 936.00 GBP - 10110.00 GBP
AlphaLISA immunoassay kits

CD80 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human CD80 enables the quantitative determination of human cluster of differentiation 80 (CD80) in serum, buffered solution, and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 470.00 GBP - 9030.00 GBP
SureFire principle

AlphaScreen SureFire ErbB2 (p-Tyr1221;Tyr1222) Assay Kit

The AlphaScreen® SureFire® p-ErbB2 (Tyr1221/1222) assay is a sandwich immunoassay for quantitative detection of phospho-ErbB2 (phosphorylated on Tyr1221/1222) in cellular lysates using Alpha Technology.
Price Range : 936.00 GBP - 10110.00 GBP
AlphaLISA immunoassay kits

IL-17A (human) AlphaLISA Biotin-Free Detection Kit

AlphaLISA Human IL-17A Biotin-Free Detection Kit is designed for the quantitative detection of human IL-17A in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin.
Price Range : 436.00 GBP - 6310.00 GBP
AlphaPlex Samarium immunoassay kits

AlphaPlex-645 (Sm) Human IL2 Detection Kit

The AlphaPlex™ 645 (Samarium) Human IL2 Detection Kit is designed for the quantitative determination of human IL-2 together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) multiplexing assay.
Price Range : 381.00 GBP - 6300.00 GBP
AlphaLISA immunoassay kits

ApoA1 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for ApoA1 is designed for the quantitative determination of ApoA1 in human serum, human plasma and cell supernantants using a homogeneous AlphaLISA assay (no wash steps). Neglible cross reactivity was observed with ApoCIII, ApoE, or ApoB.
Price Range : 388.00 GBP - 11630.00 GBP
AlphaLISA Buffer

AlphaLISA 5X Epigenetics Buffer 1

Pre-formulated buffer that can be used in histone H3 peptide-based epigenetic assays.
Price Range : 76.00 GBP - 412.00 GBP
AlphaLISA immunoassay kits

CXCL1/GROα (human) AlphaLISA Detection Kit

The AlphaLISA® Human CXCL1/GROα Detection Kit is designed for detection and quantitation of Human CXCL1/GROα in human serum, plasma, and cell culture supernatants using a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 953.00 GBP - 6300.00 GBP
AlphaLISA immunoassay kits

Fibronectin (human plasma) AlphaLISA Detection Kit

The AlphaLISA® Human Plasma Fibronectin Detection Kit is designed for detection and quantitation of human plasma fibronectin (hFN) in human serum, plasma, and, cell lysate, cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 280.00 GBP - 6110.00 GBP
AlphaLISA Cellular Epigenetics kits

AlphaLISA dimethylated-Histone H3 Lysine 36 (H3K36me2) Cellular Detection Kit

This kit is designed for the detection of di-methylated Histone H3 Lysine 36 (H3K36me2) in cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 415.00 GBP - 7400.00 GBP
AlphaLISA immunoassay kits

HIV p24 AlphaLISA Biotin-Free Detection Kit

The AlphaLISA HIV p24 Biotin-Free Detection Kit is designed for the quantitative detection of HIV p24 in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).
Price Range : 436.00 GBP - 6310.00 GBP

Anti-Human IgG4 AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to a anti-human IgG4. This bead can be used to create no-wash AlphaLISA assays for isotyping and other applications.
Price Range : 354.00 GBP - 14540.00 GBP
AlphaLISA immunoassay kits

Amyloid β 1-42 (mouse/rat) AlphaLISA Detection Kit

The AlphaLISA® Mouse/Rat Amyloid beta (Aß 1-42) Detection Kit is designed for detection and quantitation of mouse or rat Aß 1-42 in serum, cerebrospinal fluid (CSF), buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1830.00 GBP - 14280.00 GBP
AlphaLISA immunoassay kits

B7-H5/VISTA (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human V-domain Ig suppressor of T cell activation (VISTA) enables the quantitative determination of human VISTA (B7-H5, PD-1H) in serum, buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 470.00 GBP - 9030.00 GBP
AlphaLISA immunoassay kits

IL-25 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human IL-25 enables the quantitative determination of human interleukin 25 (IL-25) in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 376.00 GBP - 9030.00 GBP
AlphaLISA immunoassay kits

MMP-12 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human MMP-12 enables the detection and quantitation of human matrix metalloproteinase-12 in serum or cell culture medium using a homogeneous AlphaLISA assay (no wash steps). The kit was designed to detect both MMP12 and pro-MMP12.
Price Range : 280.00 GBP - 6110.00 GBP
AlphaLisa Analyte 2

CD80 (human), lyophilized (AlphaLISA)

Human cluster of differentiation 80 (CD80) standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human CD80 Detection Kit, but can also be ordered separately.
List Price : 253.00 GBP
AlphaPlex Terbium immunoassay kits

AlphaPlex-545 (Tb) Human ICAM-1 Detection Kit

The AlphaPlex™-545 (Tb) Human ICAM-1 Detection Kit is designed for detection and quantitation of human ICAM-1 in cell culture supernatant and human serum using a homogeneous AlphaPlex multiplexing assays (no wash steps).
Price Range : 405.00 GBP - 14280.00 GBP
AlphaLISA immunoassay kits

Cortisol (human) AlphaLISA Detection Kit

AlphaLISA no-wash immunoassay kit for detection of human cortisol in buffer or serum.
Price Range : 436.00 GBP - 6310.00 GBP

AlphaScreen FLAG (M2) Detection Kit

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Anti-FLAG conjugated AlphaScreen Acceptor beads. 
Price Range : 113.00 GBP - 8640.00 GBP

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