PerkinElmer

Cell Signaling and Pathway Analysis

OF

From receptor ligand binding to intracellular pathway analysis of phosphorylated proteins, breakthrough discoveries in cell signaling and signal transduction are coming from laboratories that maximize high-quality results while minimizing hassles.

We enable a deeper understanding and more biologically relevant results with complete cell-based and biochemical assay solutions featuring innovative reagent technologies like: AlphaLISA SureFire Ultra, AlphaLISA SureFire Ultra Multiplex, AlphaScreen SureFire, LANCE Ultra TR-FRET, and DELFIA TRF assays – not to mention our complete line of industry leading radiochemicals.

With a range of highly reliable, high-performance multimode plate readers, including the EnVision and EnSight systems, we provide access to a wide range of detection technologies for everything you need to bring about the next big breakthrough.

Kinase image web
Kinase image web

Cellular Kinase Assays

Alpha SureFire® assays are no-wash cellular kinase assays. While they exist in different formats (AlphaScreen® SureFire, AlphaLISA® SureFire Ultra, and Alpha SureFire Ultra Multiplex), all use PerkinElmer’s exclusive bead-based technology in a sandwich immunoassay format for the detection of phosphorylated proteins in cells. Alpha SureFire assays are a quantitative alternative to Western Blotting, are automation-friendly, miniaturizable, and detect both endogenous and recombinant proteins.

AlphaScreen SureFire

AlphaScreen SureFire has a rich track record of small molecule screening successes:

  • Uses protein A to capture one of the kit’s antibodies
  • 4-hour incubation protocol
  • Need to order the AlphaScreen protein A kit in addition to the AlphaScreen SureFire kit

AlphaLISA SureFire Ultra

The labeling of one of the antibodies with CaptSure, a small peptide tag, is recognized by CaptSure-antibody-coated beads, providing significant improvement over the AlphaScreen SureFire format:

  • Lower background signal, improved sensitivity, and dynamic range
  • No interference from extraneous antibodies; ideal for therapeutic antibody screening
  • AlphaLISA beads are optimal for hemoglobin-containing samples
  • Quicker assay: 2-hour incubation protocol
  • Beads are included in the kit

Alpha SureFire Ultra Multiplex

This format utilizes AlphaPlex technology to read multiple signals from the same well:

  • Improves data quality with internal controls (e.g., total protein) or gathers more data (multiplex phosphorylation events) from a single experiment, resulting in significant time and cost savings
  • Same features as the AlphaLISA SureFire Ultra, beads are included in the kit
GPCR image web
GPCR image web

GPCR Studies

We offer the widest selection of proven GPCR target solutions - beyond the ligand GPCR event to the actual assessment of the cellular-activated signal transduction cascade. Enabling you to identify, validate, screen, and profile your leads is our priority.

LANCE Ultra TR-FRET

LANCE® Ultra TR-FRET Technology provides researchers with robust and easy-to-use assays adaptable to a variety of targets on a broad range of multimode detectors, with the fastest time-to-results in a commercially-available TR-FRET assay.

  • No-wash/separation steps
  • Homogeneous dispense-and-read technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 384-well and 1536-well formats

LANCE and LANCE (LANthanide Chelate Excitation) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer) based, homogeneous (no wash) technologies. One binding partner of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and a second one is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320-340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light detectable at 665 nm.

DELFIA

DELFIA (Dissociation-Enhanced Lanthanide Fluorescence Immunoassay) is a proven, robust immunodetection platform based on time-resolved fluorescence (TRF) detection. This detection method, coupled with the unique chemical properties of lanthanide chelates, makes DELFIA a superior alternative to conventional ELISA for research, drug discovery, and clinical applications.

DELFIA offers a combination of high sensitivity, wide dynamic range and long-lasting stability that traditional ELISA cannot match.

Radiometric Detection

Radiometric detection is a direct method that removes the need for target specific antibodies, reducing assay time & variables. A range of isotopes (3H, 14C, 33P, 35S) can be labeled to a variety of without altering biological function to study in the most natural state receptor ligand binding, kinases, G proteins, and other cell signaling pathways.

For high throughput screening, SPA (Scintillation Proximity Assay) technology provides a homogenous radiometric format for a multitude of assays.

  • No separation or washing steps saves time and generates reliable results.
  • A wide range of SPA bead coatings allows a variety of targets to be assayed.
  • A more convenient, cost-effective, and safer alternative to radioactive filter binding assay
  • Amenable to automation for increased throughput.
  • SPA beads can be used in tubes, 96-, 384- or 1536-well microplates and their signal measured on any scintillation counter.
  • Assay miniaturization is possible, reducing total assay volumes and concentrations per well for lower reagents costs and higher throughput.
  • SPA beads are suitable for use with 3H-, 125I-, 33P-, 35S-, and 14C-labeled ligands.
  • No requirement for protein modification.

An alternative format, SPA Imaging Beads are designed for detection on CCD based microplate imagers They offer additional benefits to SPA Scintillation Beads, including:

  • Red-shifted signal minimizes quenching with colored compounds to reduce the number of false negatives.
  • Format-free imaging of any plate density increases throughput to produce results faster.
EnSight multimode plate-reader
EnSight multimode plate-reader

Multimode Plate Readers

We offer plate readers with a wide range of modes to meet the diverse application requirements of any Cell Signaling lab. Each multimode detection plate reader features leading technologies, so no matter which instrument you choose, you can expect excellent performance for your specific assay.
  • VICTOR X Multilabel Plate Reader - a versatile, reliable system delivering multiple detection technologies on a single cost-effective platform, providing flexibility, sensitivity, and ease of use to Cell Signalling research labs of all sizes.
  • EnSpire® Multimode Plate Reader - designed to meet the demands of multiuser research environments, providing high-performance technology and flexibility for a range of applications – without compromising sensitivity.
  • EnSpire Alpha Plate Reader - proven Alpha technology at an affordable price.
  • EnSight Multimode Plate Reader - the first benchtop system to offer well imaging alongside labeled and label-free technologies, the EnSight system’s unique combination of detection modes enables you to monitor the quality of your cell samples and take an orthogonal approach to your assays.
  • EnVision®Multilabel Plate Reader - One of the fastest readers in the market, the EnVision® multilabel system includes two detectors enabling simultaneous, fast dual-wavelength reading and high-speed, sensitive laser-based TRF and Alpha technology detection. And it’s expandable, too, as your throughput and application needs change and grow.
  • ViewLux® - the top-of-the-line microplate imager that’s ideal for ultrahigh-throughput screening of compound libraries requiring the ultimate in speed, reliability, and high-quality data.
  • The MicroBeta2 System provides advanced radiometric and luminescent detection abilities for GPCRs, kinases, reporter gene assays, and traditional liquid scintillation counting. Providing coincidence counting, a unique patented configuration with two photomultiplier tubes that simultaneously detect signal, this system ensures high efficiency and extremely low background for a variety of radionuclides.
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"Cell Signaling and Pathway Analysis" 1-50 of 165 Products and Services

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EnVision_Alone_Left_Angle

EnVision Multilabel Plate Reader

The EnVision Multilabel Plate Reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. Tried and tested, the EnVision microplate reader gives you robust performance and reliable data for high throughput screening, time after time.
EnSight multimode plate-reader

EnSight Multimode Plate Reader

The EnSight multimode plate reader combines labeled, label-free* & image-based cytometry technologies in a single benchtop platform. Imaging & online data analysis is fast: a 384-well plate can be imaged in less than 5 mins. The system’s modular design, combined with workflow-based Kaleido data acquisition & analysis software, gives a truly versatile plate reader that gets users productive quickly.

*Label free detection module of EnSight Plate Reader is available outside the EU. Please contact your local representative for further details.

SureFire principle

AlphaScreen SureFire cJun (p-Ser63) Assay Kit

The AlphaScreen® SureFire® p-cJun (Ser63) assay is a sandwich immunoassay for quantitative detection of phospho-cJun (phosphorylated on Ser63) in cellular lysates using Alpha Technology.
Price Range : 936.00 GBP - 10110.00 GBP
alphascreen surefire total

AlphaScreen SureFire Akt 1/2/3 Total Assay Kit

The AlphaScreen® SureFire® Akt 1/2/3 (total) assay is a sandwich immunoassay for quantitative detection of Akt 1/2/3 in cellular lysates using Alpha Technology. The assay is an ideal system for normalizing Akt levels for experiments measuring changes in Akt phosphorylation.
Price Range : 256.00 GBP - 10110.00 GBP
SureFire principle

AlphaScreen SureFire Phospho-Caspase 9 (Ser196) Kit

The AlphaScreen® SureFire® p-Caspase 9 (Ser196) assay is a sandwich immunoassay for quantitative detection of phospho-Caspase 9 (phosphorylated on Ser196) in cellular lysates using Alpha Technology
Price Range : 936.00 GBP - 4120.00 GBP
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra AKT 1/2/3 (pS473) Assay Kit

The AlphaLISA® SureFire® Ultra p-AKT1/2/3 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-AKT1/2/3 (phosphorylated on Ser473) in cellular lysates using Alpha Technology.
Price Range : 307.00 GBP - 17790.00 GBP
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

Phospho-SMAD1 (Ser463/465) LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra phospho-SMAD1 (Ser463/465) kits are designed for the detection of SMAD1 (phosphorylated at S463/465) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
Price Range : 892.00 GBP - 4000.00 GBP
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-ERK1/2 (Thr202/Tyr204) Assay Kit

The AlphaLISA® SureFire® Ultra p-ERK 1/2 (Thr202/Tyr204) assay is a sandwich immunoassay for quantitative detection of phospho-ERK 1/2 (phosphorylated on Thr202/Tyr204 in ERK1, or Thr185/Tyr187 in ERK2) in cellular lysates using Alpha Technology.
Price Range : 307.00 GBP - 17790.00 GBP
SureFire principle

AlphaScreen SureFire Phospho-c-Met (Tyr1234/1235) Assay Kit 

The AlphaScreen® SureFire® p-c-Met (Tyr1234/1235) assay is a sandwich immunoassay for quantitative detection of phospho-c-Met (phosphorylated on Tyr1234/1235) in cellular lysates using Alpha Technology.
Price Range : 936.00 GBP - 10120.00 GBP
alphascreen surefire total

AlphaScreen SureFire Total STAT3 Assay Kit

The AlphaScreen® SureFire® total STAT3 assay is a sandwich immunoassay for quantitative detection of STAT3 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
Price Range : 256.00 GBP - 10120.00 GBP
SureFire principle

AlphaScreen SureFire CREB (p-Ser133) Assay Kit

The AlphaScreen® SureFire® p-CREB (Ser133) assay is a sandwich immunoassay for quantitative detection of phospho-CREB (phosphorylated on Ser133) in cellular lysates using Alpha Technology.
Price Range : 1030.00 GBP - 11090.00 GBP
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-SMAD1 (Ser463/465) Assay Kit

The AlphaLISA® SureFire® Ultra p-SMAD1 (Ser463/Ser465) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD1 (phosphorylated on Ser463/Ser465) in cellular lysates using Alpha Technology.
Price Range : 307.00 GBP - 17790.00 GBP
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-eIF2α (Ser51) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-eIF2α (Ser51) assay is a sandwich immunoassay for quantitative detection of phospho-eIF2α (phosphorylated on Ser51) in cellular lysates using Alpha Technology.
Price Range : 307.00 GBP - 17790.00 GBP
SureFire principle

AlphaScreen SureFire Phospho-cofilin (Ser3) Assay Kit

The AlphaScreen® SureFire® p-Cofilin (Ser3) assay is a sandwich immunoassay for quantitative detection of phospho-Cofilin (phosphorylated on Ser3) in cellular lysates using Alpha Technology.
Price Range : 936.00 GBP - 10120.00 GBP
SureFire principle

AlphaScreen SureFire JNK 1-3 (p-Thr183;Tyr185) Assay Kit

The AlphaScreen® SureFire® p-JNK1/3 (Thr183/Tyr185) assay is a sandwich immunoassay for quantitative detection of phospho-JNK1/3 (phosphorylated on Thr183/Tyr185) in cellular lysates using Alpha Technology.
Price Range : 936.00 GBP - 10110.00 GBP
SureFire principle

AlphaScreen SureFire IkBalpha (p-Ser32/Ser36) Assay Kit

The AlphaScreen® SureFire® p-IκBα (Ser32/36) assay is a sandwich immunoassay for quantitative detection of phospho-IκBα (phosphorylated on Ser32/36) in cellular lysates using Alpha Technology.
Price Range : 936.00 GBP - 10110.00 GBP
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-VEGF Receptor 2 (Tyr1175) Assay Kit

The AlphaLISA® SureFire® Ultrap-VEGF Receptor 2 (Tyr1175) assay is a sandwich immunoassay for quantitative detection of phospho-VEGFR Receptor 2 (phosphorylated on Tyr1175) in cellular lysates using Alpha Technology.
Price Range : 307.00 GBP - 17790.00 GBP
alphascreen surefire total

AlphaScreen SureFire Monoclonal Total ERK, 50,000 Assay Points

The AlphaScreen® SureFire® total ERK assay is a sandwich immunoassay for quantitative detection of ERK (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies. This assay performs similarly to the TGRTES SureFire kit, but contains a monoclonal antibody for ERK detection.
List Price : 11110.00 GBP
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

Phospho-ERK 1/2 (Thr202/Tyr204) LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra phospho-ERK 1/2 (Thr202/Tyr204) kits are designed for the detection of phosphorylated ERK 1 and ERK2 in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
Price Range : 892.00 GBP - 4000.00 GBP
SureFire principle

AlphaScreen SureFire Phospho-ATF2 (Thr71) Assay Kit

The AlphaScreen® SureFire® p-ATF2 (Thr71) assay is a sandwich immunoassay for quantitative detection of phospho-ATF2 (phosphorylated on Thr71) in cellular lysates using Alpha Technology.
Price Range : 936.00 GBP - 10120.00 GBP
SureFire principle

AlphaScreen SureFire p53 (p-Ser15) Assay Kit

The AlphaScreen® SureFire® p-p53 (p-Ser15) assay is a sandwich immunoassay for quantitative detection of phospho-p53 (phosphorylated on Ser15) in cellular lysates using Alpha Technology.
Price Range : 256.00 GBP - 10110.00 GBP
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-Ribosomal Protein S6 (Ser240/244) Assay Kit, 500 Assay Points

The AlphaLISA® SureFire® Ultra™ p-rpS6 (Ser240/244) assay is a sandwich immunoassay for quantitative detection of phospho-Ribosomal Protein S6 (phosphorylated on Ser240/244) in cellular lysates using Alpha no-wash technology.
List Price : 1040.00 GBP
SureFire principle

AlphaScreen SureFire Phospho-GSK 3a (Ser21) Kit

The AlphaScreen® SureFire® p-GSK 3α (Ser21) assay is a sandwich immunoassay for quantitative detection of phospho-GSK3α (phosphorylated on Ser21) in cellular lysates using Alpha Technology.
Price Range : 936.00 GBP - 10110.00 GBP
SureFire principle

AlphaScreen SureFire S6RP (p-Ser240;Ser244) Assay Kit

The AlphaScreen® SureFire® p-Ribosomal Protein S6 (p-Ser240/244) assay is a sandwich immunoassay for quantitative detection of phospho-RP S6 (phosphorylated on Ser240/244) in cellular lysates using Alpha Technology.
Price Range : 936.00 GBP - 10110.00 GBP
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-IGF-1 Receptor β (Tyr1135/1136) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-IGF-1 Receptor β (Tyr1135/1136) assay is a sandwich immunoassay for quantitative detection of phospho-IGF Receptor β (phosphorylated on Tyr1135/1136) in cellular lysates using Alpha Technology.
Price Range : 307.00 GBP - 17790.00 GBP
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

Phospho-STAT3 (Tyr705) LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra phospho-STAT3 (Tyr705) kits are designed for the detection of phosphorylated STAT3 in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
Price Range : 892.00 GBP - 4000.00 GBP
alphascreen surefire total

AlphaScreen SureFire GAPDH Assay Kit

The AlphaScreen® SureFire® total GAPDH assay is a sandwich immunoassay for quantitative detection of GAPDH in cellular lysates using Alpha Technology. This assay is intended to be used as a cellular normalization assay.
Price Range : 1030.00 GBP - 11100.00 GBP
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-SMAD3 (Ser423/425) Assay Kit

The AlphaLISA® SureFire® Ultra p-SMAD3 (Ser423/Ser425) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD3 (phosphorylated on Ser423/Ser425) in cellular lysates using Alpha Technology.
Price Range : 307.00 GBP - 17790.00 GBP
alphascreen surefire total

AlphaScreen SureFire Monoclonal Total ERK, 10,000 Assay Points

The AlphaScreen® SureFire® total ERK assay is a sandwich immunoassay for quantitative detection of ERK (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies. This assay performs similarly to the TGRTES SureFire kit, but contains a monoclonal antibody for ERK detection.
List Price : 4550.00 GBP
SureFire principle

AlphaScreen SureFire Histone H3 (p-Ser10) Assay Kit

The AlphaScreen® SureFire® p-Histone H3 (Ser10) assay is a sandwich immunoassay for quantitative detection of phospho-Histone H3 (phosphorylated on Ser10) in cellular lysates using Alpha Technology.
Price Range : 936.00 GBP - 10110.00 GBP
SureFire principle

AlphaScreen SureFire Phospho-MEK 1 Kit

The AlphaScreen® SureFire® p-MEK1 (Ser218/222) assay is a sandwich immunoassay for quantitative detection of phospho-MEK1 (phosphorylated on Ser218/222) in cellular lysates using Alpha Technology.
Price Range : 936.00 GBP - 10110.00 GBP
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular detection assay

Total SLP-76 LANCE Ultra TR-FRET Cellular Detection Kit, 10,000 Assay Points

LANCE Ultra total SLP-76 kits are designed for the detection of SLP-76 (phosphorylated and non-phosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay can be used as a normalizaton assay for phospho-SLP-76, and is compatible with both adherent and suspension cells.
List Price : 4000.00 GBP
SureFire principle

AlphaScreen SureFire InsulinR (p-Tyr1150;Tyr1151) Assay Kit

The AlphaScreen® SureFire® p-Insulin Receptor (Tyr1150/1151) assay is a sandwich immunoassay for quantitative detection of phospho-Insulin Receptor (phosphorylated on Tyr1150/1151) in cellular lysates using Alpha Technology.
Price Range : 1030.00 GBP - 11100.00 GBP
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra Total Akt1 Assay Kit

The AlphaLISA® SureFire® Ultra™ Total Akt1 assay is a sandwich immunoassay for quantitative detection of Akt1 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
Price Range : 307.00 GBP - 17790.00 GBP
SureFire principle

AlphaScreen SureFire p-Akt 1/2/3 (Thr308) research kit

The AlphaScreen® SureFire® p-Akt1/2/3 (Thr308) assay is a sandwich immunoassay for quantitative detection of phospho-Akt1/2/3 (phosphorylated on Thr308) in cellular lysates using Alpha Technology.
Price Range : 1030.00 GBP - 11100.00 GBP
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra Total STAT5 Assay Kit - 500 Assay Points

The AlphaLISA® SureFire® Ultra™ Total STAT5 assay is a sandwich immunoassay for quantitative detection of STAT5 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
List Price : 1040.00 GBP
alphascreen surefire total

AlphaScreen SureFire p53 Total Assay Kit

The AlphaScreen® SureFire® total p53 assay is a sandwich immunoassay for quantitative detection of p53 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
Price Range : 256.00 GBP - 10110.00 GBP
SureFire principle

AlphaScreen SureFire EIF4E (p-Ser209) Assay Kit

The AlphaScreen® SureFire® p-eIF4E (Ser209) assay is a sandwich immunoassay for quantitative detection of phospho-eIF4E (phosphorylated on Ser209) in cellular lysates using Alpha Technology.
Price Range : 1030.00 GBP - 11100.00 GBP
alphascreen surefire total

AlphaScreen SureFire Total NFkB p65 Assay Kit

The AlphaScreen® SureFire® total NFκB assay is a sandwich immunoassay for quantitative detection of NFκB (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
Price Range : 256.00 GBP - 10120.00 GBP
SureFire principle

AlphaScreen SureFire p-VEGF2 Receptor (Tyr1175) research kit

The AlphaScreen® SureFire® p-VEGFR2 Receptor (p-Tyr1175) assay is a sandwich immunoassay for quantitative detection of phospho-VEGF2 Receptor (phosphorylated on Tyr1175) in cellular lysates using Alpha Technology.
Price Range : 1030.00 GBP - 11100.00 GBP
SureFire principle

AlphaScreen SureFire Monoclonal ERK p-T202/Y204, 10,000 Assay Points

The AlphaScreen® SureFire® Monoclonal p-ERK1/2 (Thr202/Tyr204) assay is a sandwich immunoassay for quantitative detection of phospho-ERK1/2 (phosphorylated on Thr202/Tyr204) in cellular lysates using Alpha Technology. This assay performs similarly to the TGRES SureFire kit, but contains a monoclonal antibody for phospho-ERK detection.
List Price : 4550.00 GBP
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular detection assay

Total ERK 1/2 LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra Total ERK 1/2 kits are designed for the detection of total (phosphorylated and non-phosphorylated) ERK1 and ERK2 in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay can be used as a normalization assay for phospho-ERK detection, and is compatible with both adherent and suspension cells.
Price Range : 892.00 GBP - 4000.00 GBP
SureFire principle

AlphaScreen SureFire Phospho-GSK 3b (Ser9) Kit

The AlphaScreen® SureFire® p-GSK 3β (Ser9) assay is a sandwich immunoassay for quantitative detection of phospho-GSK3β (phosphorylated on Ser9) in cellular lysates using Alpha Technology.
Price Range : 936.00 GBP - 10110.00 GBP
SureFire principle

AlphaScreen SureFire Akt 1 (p-Ser473) Assay Kit

The AlphaScreen® SureFire® p-Akt1 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-Akt1 (phosphorylated on Ser473) in cellular lysates using Alpha Technology.
Price Range : 936.00 GBP - 10110.00 GBP
alphascreen surefire total

AlphaScreen SureFire Total IkB alpha Assay Kit

The AlphaScreen® SureFire® total IκBαassay is a sandwich immunoassay for quantitative detection of IκBα(both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
Price Range : 936.00 GBP - 10120.00 GBP

LANCE cAMP Detection Kit

LANCE® TR-FRET cAMP assay kit, for Gs- and Gi-coupled GPCR functional assays.
Price Range : 100.00 GBP - 4450.00 GBP
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

Phospho-AKT 1/2/3 (Ser473) LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra phospho-AKT1/2/3 (Ser473) kits are designed for the detection of AKT1, AKT2, and AKT3 (phosphorylated at residue S473) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
Price Range : 892.00 GBP - 4000.00 GBP
SureFire principle

AlphaScreen SureFire ALK (p-Tyr1586) Assay Kit

The AlphaScreen® SureFire® p-ALK (Tyr1586) assay is a sandwich immunoassay for quantitative detection of phospho-ALK (phosphorylated on Tyr1586) in cellular lysates using Alpha Technology.
Price Range : 936.00 GBP - 10110.00 GBP
SureFire principle

AlphaScreen SureFire Phospho-Caspase 9 (Ser196) Kit for 50,000 Assay Points

The AlphaScreen® SureFire® p-Caspase 9 (Ser196) assay is a sandwich immunoassay for quantitative detection of phospho-Caspase 9 (phosphorylated on Ser196) in cellular lysates using Alpha Technology
List Price : 10110.00 GBP
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

Phospho-SLP-76 (Ser376) LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra phospho-SLP-76 (Ser376) kits are designed for the detection of SLP76 (phosphorylated at S376) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
Price Range : 892.00 GBP - 4000.00 GBP

"Cell Signaling and Pathway Analysis" 1-50 of 165 Products and Services

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