PerkinElmer

Drug Discovery Reagents

OF

Drug discovery is a complex, costly, and time-consuming undertaking. And for research scientists, high-throughput screening is where the journey to discovery begins.

Your job is to move from target to hit to candidate quickly and efficiently – with as little wasted resources as possible. Our HTS screening reagents do just that, providing you with a wide range of validated reagents and kits in Alpha, LANCE®, DELFIA®, and other assay technologies.

Accelerate your workflow, while getting consistent, accurate, and physiologically relevant results.

AlphaLISA assay principle
AlphaLISA assay principle

Alpha Technology

Virtually anything can be measured with Alpha Technology quickly and easily, including enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein:protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous, no-wash assay, with no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerbrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the Alpha solution that’s right for you

  • AlphaLISA® – when running large numbers of ELISA from the same sample or working with difficult matrix, serum or plasma
  • AlphaLISA SureFire Ultra and AlphaScreen SureFire – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaScreen® – perfect for studying fusion-tagged proteins or protein: protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf kit, the toolbox gives you everything you need to create your own
LANCE biomarker detection assay principle
LANCE biomarker detection assay principle

LANCE Ultra TR-FRET

From assay development to primary and secondary screening, LANCE Ultra Technology provides researchers with robust and easy-to-use assays adaptable to a variety of targets on a broad range of multimode detectors, with the fastest time-to-results in a commercially-available TR-FRET assay.

  • No-wash/separation steps
  • Homogeneous dispense-and-read technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 384-well and 1536-well formats

LANCE and LANCE (LANthanide Chelate Excitation) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One binding partner of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and a second one is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320-340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light detectable at 665 nm.

DELFIA_trf
DELFIA_trf

DELFIA

DELFIA (Dissociation-Enhanced Lanthanide Fluorescence Immunoassay) is a proven, robust immunodetection platform based on time-resolved fluorescence (TRF) detection. This detection method, coupled with the unique chemical properties of lanthanide chelates, makes DELFIA a superior alternative to conventional ELISA for research, drug discovery, and clinical applications.

DELFIA offers a combination of high sensitivity, wide dynamic range and long-lasting stability that traditional ELISA cannot match. 

Reporter gene assay
Reporter gene assay

Reporter Gene Assays

Whether your assay requires the sensitivity provided by high signal intensity, or the flexibility of an extended signal half-life, PerkinElmer provides a solution. Choose from one of three luciferase detection systems: neolite, britelite plus, or steadylite plus depending on the specific requirements of your experiment.
  • No more washing – just add your lites reagent of choice, mix, incubate, and read
  • Hassle-free storage – all lites reagents are designed for extended storage at 2-8 °C (no -20 °C storage required)
  • Odor-friendly, eliminates hood work – lites products do not contain DTT and do not have the hazards and repugnant odor typical of conventional luciferase detection systems
  • Excellent Z’ values – superior S/B ratios allow for excellent Z’ values in antagonist and agonist studies
ATPlite assay principle
ATPlite assay principle

ATP Quantitation

For fast, reliable detection of ATP for cell quantitation, ATPlite 1step and ATPlite are innovative technologies that measure cell proliferation and cytotoxicity in mammalian cells based on the detection of ATP using firefly luciferase.

ATPlite 1step – Highest sensitivity

  • True “mix and measure” continuous processing
  • Ultra high sensitivity, 3 times more light output than ATPlite
  • Short equilibrium time for faster processing
  • Signal half-life of 30 minutes

ATPlite – Convenient, extended Signal

  • 2-step format, non-separation assay for time course studies; lysate can be stored frozen and tested later
  • Quantitate from <5 cells/well
  • Prolonged equilibrium time for batch processing
  • Signal half-life of 5 hours
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AlphaLISA immunoassay kits

TNFα (porcine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for porcine TNFα enables the detection and quantitation of porcine TNFα in porcine serum, plasma, and, cell lysyate, cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows minimal cross-reactivity with human and bovine TNFα and no cross reactivity towards porcine IFNγ.
Price Range : 402.00 GBP - 13070.00 GBP

LANCE Ultra Europium-anti-di-methyl-Histone H3 Lysine 36 (H3K36me2) Antibody

Europium-labeled monoclonal antibody recognizing human Histone H3 di-methylated at lysine 36 (H3K36me2). For use in LANCE® Ultra epigenetic writer and eraser assays.
Price Range : 2190.00 GBP - 5360.00 GBP
AlphaLISA immunoassay kits

Alpha-synuclein (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human Alpha-synuclein enables the quantitative determination of Alpha-synuclein in biological fluids such as serum, plasma, cerebrospinal fluid and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 417.00 GBP - 6530.00 GBP
AlphaLisa Analyte 2

IL-13 (porcine), lyophilized (AlphaLISA)

Porcine IL-13 standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA IL-13 Detection Kit, but can be ordered separately.
List Price : 155.00 GBP

GLP-1 (7-36) (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human GLP-1 (7-36) Detection Kit is designed for detection and quantitation of human GLP-1 (7-36) in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 514.00 GBP - 6860.00 GBP
AlphaLisa Analyte 2

Alpha-synuclein (human), lyophilized (AlphaLISA)

Human Alpha-synuclein standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human Alpha-synuclein detection kit, but can also be ordered separately.
List Price : 160.00 GBP
AlphaLisa Analyte 2

IGF-1 (rat), lyophilized (AlphaLISA)

Rat IGF-1 standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA Rat IGF-1 Detection Kit, but can also be ordered separately.
List Price : 191.00 GBP
AlphaLISA immunoassay kits

AST (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human AST enables the quantitative determination of human aspartate transaminase (AST) in buffered solution, cell culture media, and serum using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 470.00 GBP - 9030.00 GBP
PerkinElmer

LANCE Ultra ULight™-PLK (Ser137) peptide

ULight™-labeled synthetic peptide containing the amino acid residues surrounding Ser137 of polo-like kinase (PLK). The ULight-PLK peptide can be phosphorylated at Ser137 by a variety of kinases including Aurora A, PAK2, MSK1, PKA and PKC. Peptide core motif : RRRSLLE
Price Range : 142.00 GBP - 516.00 GBP
AlphaLISA immunoassay kits

IP-10 (human) AlphaLISA Detection Kit

The AlphaLISA® Human Interferon Gamma-induced Protein 10 (hIP10) Detection Kit is designed for the quantitative determination of human IP10 in serum, plasma, and cell culture supernatants using a homogeneous (no wash steps, no separation steps) assay.
Price Range : 449.00 GBP - 6490.00 GBP

Frataxin (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human Frataxin Detection Kit is designed for detection and quantitation of human frataxin in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 514.00 GBP - 6860.00 GBP

Lens culinaris agglutinin (LCA) AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to LCA (Lens culinaris agglutinin). These beads can be used to capture glycoproteins containing α1,6-linked fucose, and can be used in conjunction with Alpha Donor beads to create AlphaLISA no-wash assays for detection of fucosylated proteins.
Price Range : 368.00 GBP - 15120.00 GBP
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-ERK1/2 (Thr202/Tyr204) Assay Kit

The AlphaLISA® SureFire® Ultra p-ERK 1/2 (Thr202/Tyr204) assay is a sandwich immunoassay for quantitative detection of phospho-ERK 1/2 (phosphorylated on Thr202/Tyr204 in ERK1, or Thr185/Tyr187 in ERK2) in cellular lysates using Alpha Technology.
Price Range : 316.00 GBP - 18330.00 GBP
AlphaLISA immunoassay kits

IL-6 (monkey) AlphaLISA Detection Kit, 100 Assay Points

The AlphaLISA® immunoassay kit for monkey IL-6 enables the quantitative determination of cynomolgus monkey interleukin-6 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 388.00 GBP - 11630.00 GBP
AlphaLISA immunoassay kits

IGF-1 (rat) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for rat IGF-1 enables the quantitative determination of rat insulin-like growth factor 1 (IGF-1) in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 388.00 GBP - 11630.00 GBP
AlphaLISA immunoassay kits

HLA-C (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human HLA-C enables the quantitative determination of human MHC class I antigen C (HLA-C) in buffered solution, cell culture media, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 485.00 GBP - 9300.00 GBP
AlphaLISA assay principle

IL-23 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human IL-23 enables the quantitative determination of human interleukin-23 in human serum, plasma, cell lysates, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 399.00 GBP - 11980.00 GBP
AlphaLisa Analyte 2

IL-2 (mouse), lyophilized (AlphaLISA)

Mouse Interleukin-2 (IL-2) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Mouse IL-2 kit, but can be ordered separately.
List Price : 159.00 GBP
PerkinElmer

AlphaLISA BRD4_BD1 / H4 K5,8,12,16Ac Binding Kit

This kit is designed for the detection of binding activity between bromodomain-containing protein 4, Bromodomain1: BRD4 (BD1) and acetylated histone H4 peptide (H4 K5,8,12,16Ac) using a homogeneous AlphaLISA assay (no wash steps). The assay allows screening of BRD4 inhibitors with a highly-specific substrate.
List Price : 505.00 GBP
Protein

IL-2 (human), lyophilized (LANCE)

Human IL-2 standard, for running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra human IL-2 detection kit, but can also be ordered separately.
List Price : 160.00 GBP

Anti-Human IgG4 AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to a anti-human IgG4. This bead can be used to create no-wash AlphaLISA assays for isotyping and other applications.
Price Range : 365.00 GBP - 14980.00 GBP
Protein

C-peptide (mouse), lyophilized (LANCE)

Mouse C-peptide standard, lyophilized, for use in running standard curves in LANCE TR-FRET assays. This standard is already provided in the LANCE Ultra C-peptide TR-FRET Detection Kit, but can be ordered separately.

 

List Price : 342.00 GBP
AlphaLISA immunoassay kits

IFNγ (human) AlphaLISA Biotin-Free Detection Kit

The AlphaLISA Human IFNγ Biotin-Free Detection Kit is designed for the quantitative detection of human IFNγ in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).
Price Range : 449.00 GBP - 6500.00 GBP

Host Cell Residual DNA contamination LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Residual DNA Contamination Detection Kit is designed for detection and quantitation of DNA contaminants in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 499.00 GBP - 6660.00 GBP
AlphaLisa Analyte 2

IL-22 (human), lyophilized (AlphaLISA)

Human IL-22 standard, 1 µg, for use in AlphaLISA immunoassays. This vial contains enough analyte to run 10 standard curves. This standard is provided in the AlphaLISA human/rat IL-22 detection kits, but can also be ordered separately.
List Price : 160.00 GBP

Anti-IgG1 AlphaLISA Acceptor beads

AlphaLISA® Acceptor beads conjugated to anti-human IgG1. These beads can be used in conjunction with Alpha Donor beads to create AlphaLISA no-wash assays for antibody binding studies, pharmacokinetics (PK), or detection of human IgG1 in different sample matrices, including monkey serum.
Price Range : 368.00 GBP - 15120.00 GBP
AlphaLISA immunoassay kits

Fibrinogen (human) AlphaLISA Detection Kit

The AlphaLISA® Human Fibrinogen Detection Kit is designed for detection and quantitation of Human fibrinogen in human serum, plasma, and cell culture supernatants using a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 288.00 GBP - 6490.00 GBP
SureFire principle

AlphaScreen SureFire JNK 123 (p-Thr183;Tyr185) Assay Kit

The AlphaScreen® SureFire® p-JNK1/2/3 (Thr183/Tyr185) assay is a sandwich immunoassay for quantitative detection of phospho-JNK1/2/3 (phosphorylated on Thr183/Tyr185) in cellular lysates using Alpha Technology.
Price Range : 1070.00 GBP - 11430.00 GBP
AlphaLISA immunoassay kits

CXCL10/IP-10 (mouse) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for mouse Interferon Gamma Induced Protein 10 (mIP-10)/CXCL10 enables the quantitative determination of mouse IP-10 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 280.00 GBP - 6120.00 GBP

CXCL1/GRO-α (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human CXCL1/GRO-α Detection Kit is designed for detection and quantitation of human CXCL1 (GRO-α) in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 514.00 GBP - 6830.00 GBP
AlphaLISA immunoassay kits

IL-13 (porcine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for IL13 is designed for the quantitative determination of IL13 in porcine serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows minimal cross-reactivity with human IL13.
Price Range : 450.00 GBP - 6680.00 GBP
AlphaPlex-545-antibody-bead2

Anti-FLAG AlphaPlex-545 (Tb) Acceptor beads

AlphaPlex-545 (Terbium) Acceptor beads conjugated to anti-FLAG antibody, for use in Alpha no-wash multiplexing assays.
Price Range : 380.00 GBP - 15580.00 GBP
alphascreen surefire total

AlphaScreen SureFire Total NFkB p65 Assay Kit

The AlphaScreen® SureFire® total NFκB assay is a sandwich immunoassay for quantitative detection of NFκB (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
Price Range : 264.00 GBP - 10430.00 GBP
AlphaLisa Analyte 2

CYR61 (human), lyophilized (AlphaLISA)

Human cysteine rich angiogenic inducer 61 (CYR61) standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA Human Cyr61 Detection Kit, but can also be ordered separately.
List Price : 485.00 GBP

DELFIA Assay Buffer

DELFIA® Assay Buffer is a buffered protein and detergent solution intended for use in DELFIA time-resolved fluorescence (TRF) assays.
Price Range : 23.00 GBP - 426.00 GBP
AlphaLISA immunoassay kits

IgA (mouse) AlphaLISA Detection Kit

The AlphaLISA immunoassay kit for detection and quantitation of mouse IgA in cell culture/non-mouse serum allows for fast, reproducible, and sensitive detection without the need for time-consuming wash steps or complicated assay development.
Price Range : 400.00 GBP - 6240.00 GBP
Protein

IL-17A (human), lyophilized (LANCE)

Human IL-17A standard, lyophilized, for use in running standard curves in LANCE TR-FRET detection assays. This standard is already provided in the LANCE Ultra Human IL-17A Detection Kit, but can be ordered separately.
List Price : 155.00 GBP
AlphaLISA immunoassay kits

ApoE (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for ApoE enables the quantitative determination of Apolipopriein E (ApoE) in human serum, plasma, cell lysate, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows minimum cross-reactivity with ApoA1, ApoB, and ApoCIII. Crossreactivity with other species has not tested.
Price Range : 399.00 GBP - 11980.00 GBP
AlphaLISA immunoassay kits

FLT-1/VEGFR 1 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human VEGFR 1 (FLT-1) enables the quantitative determination of VEGFR 1 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 280.00 GBP - 6120.00 GBP

LANCE Ultra Europium-anti-acetyl-p53 Lysine 382 (p53K382ac) Antibody

Europium-labeled monoclonal antibody recognizing human p53 acetylated at lysine 382 (p53K382ac). For use in LANCE® Ultra epigenetic writer and eraser assays.
Price Range : 2140.00 GBP - 5360.00 GBP

AlphaLISA Biotinylated Anti-Histone H3 (C-ter) Antibody

Biotinylated antibody against the C-terminal end of human histone H3, for use in Alpha no-wash epigenetic assays using full-length histone H3 as substrate.
Price Range : 681.00 GBP - 2410.00 GBP

Fibrinogen (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human Fibrinogen Detection Kit is designed for detection and quantitation of human fibrinogen in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 499.00 GBP - 6660.00 GBP
AlphaLISA immunoassay kits

TNFα (human) AlphaLISA Biotin-Free Detection Kit

The AlphaLISA Human TNFα Biotin-Free Detection Kit is designed for the quantitative detection of human TNFα in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).
Price Range : 449.00 GBP - 6500.00 GBP
alphascreen surefire total

AlphaScreen SureFire Akt 1/2/3 Total Assay Kit

The AlphaScreen® SureFire® Akt 1/2/3 (total) assay is a sandwich immunoassay for quantitative detection of Akt 1/2/3 in cellular lysates using Alpha Technology. The assay is an ideal system for normalizing Akt levels for experiments measuring changes in Akt phosphorylation.
Price Range : 264.00 GBP - 10430.00 GBP

EGF (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human EGF Detection Kit is designed for detection and quantitation of human epidermal growth factor in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 514.00 GBP - 6860.00 GBP
AlphaLISA immunoassay kits

CHO-P AlphaLISA Detection Kit

AlphaLISA® no-wash immunoassay kit for detection of Chinese Hamster Ovary Host Cell Proteins (CHO HCP) in buffered solution or cell culture medium.
Price Range : 491.00 GBP - 4090.00 GBP
AlphaLISA immunoassay kits

CTLA-4 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) enables the quantitative determination of human CTLA-4 in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 388.00 GBP - 9300.00 GBP
Alpha-Unconjugated-Donor-bead

AlphaScreen Unconjugated Donor Beads

Alpha Unconjugated Donor beads are provided so that you can conjugate your biomolecule-of-interest (antibody, protein, peptide, etc.) to create your own Alpha Donor beads for use in Alpha no-wash assays.
Price Range : 233.00 GBP - 6520.00 GBP
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-eIF2α (Ser51) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-eIF2α (Ser51) assay is a sandwich immunoassay for quantitative detection of phospho-eIF2α (phosphorylated on Ser51) in cellular lysates using Alpha Technology.
Price Range : 316.00 GBP - 18330.00 GBP
SureFire principle

AlphaScreen SureFire MKK3 (p-Ser189), MKK6 (p-Ser207) Assay Kit

The AlphaScreen® SureFire® p-MKK3 (Ser207) assay is a sandwich immunoassay for quantitative detection of phospho-MKK3 (phosphorylated on Ser207) in cellular lysates using Alpha Technology.
Price Range : 965.00 GBP - 10430.00 GBP

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