PerkinElmer

Drug Discovery Reagents

OF

Drug discovery is a complex, costly, and time-consuming undertaking. And for research scientists, high-throughput screening is where the journey to discovery begins.

Your job is to move from target to hit to candidate quickly and efficiently – with as little wasted resources as possible. Our HTS screening reagents do just that, providing you with a wide range of validated reagents and kits in Alpha, LANCE®, DELFIA®, and other assay technologies.

Accelerate your workflow, while getting consistent, accurate, and physiologically relevant results.

AlphaLISA assay principle
AlphaLISA assay principle

Alpha Technology

Virtually anything can be measured with Alpha Technology quickly and easily, including enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein:protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous, no-wash assay, with no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerbrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the Alpha solution that’s right for you

  • AlphaLISA® – when running large numbers of ELISA from the same sample or working with difficult matrix, serum or plasma
  • AlphaLISA SureFire Ultra and AlphaScreen SureFire – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaScreen® – perfect for studying fusion-tagged proteins or protein: protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf kit, the toolbox gives you everything you need to create your own
LANCE biomarker detection assay principle
LANCE biomarker detection assay principle

LANCE Ultra TR-FRET

From assay development to primary and secondary screening, LANCE Ultra Technology provides researchers with robust and easy-to-use assays adaptable to a variety of targets on a broad range of multimode detectors, with the fastest time-to-results in a commercially-available TR-FRET assay.

  • No-wash/separation steps
  • Homogeneous dispense-and-read technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 384-well and 1536-well formats

LANCE and LANCE (LANthanide Chelate Excitation) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One binding partner of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and a second one is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320-340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light detectable at 665 nm.

DELFIA_trf
DELFIA_trf

DELFIA

DELFIA (Dissociation-Enhanced Lanthanide Fluorescence Immunoassay) is a proven, robust immunodetection platform based on time-resolved fluorescence (TRF) detection. This detection method, coupled with the unique chemical properties of lanthanide chelates, makes DELFIA a superior alternative to conventional ELISA for research, drug discovery, and clinical applications.

DELFIA offers a combination of high sensitivity, wide dynamic range and long-lasting stability that traditional ELISA cannot match. 

Reporter gene assay
Reporter gene assay

Reporter Gene Assays

Whether your assay requires the sensitivity provided by high signal intensity, or the flexibility of an extended signal half-life, PerkinElmer provides a solution. Choose from one of three luciferase detection systems: neolite, britelite plus, or steadylite plus depending on the specific requirements of your experiment.
  • No more washing – just add your lites reagent of choice, mix, incubate, and read
  • Hassle-free storage – all lites reagents are designed for extended storage at 2-8 °C (no -20 °C storage required)
  • Odor-friendly, eliminates hood work – lites products do not contain DTT and do not have the hazards and repugnant odor typical of conventional luciferase detection systems
  • Excellent Z’ values – superior S/B ratios allow for excellent Z’ values in antagonist and agonist studies
ATPlite assay principle
ATPlite assay principle

ATP Quantitation

For fast, reliable detection of ATP for cell quantitation, ATPlite 1step and ATPlite are innovative technologies that measure cell proliferation and cytotoxicity in mammalian cells based on the detection of ATP using firefly luciferase.

ATPlite 1step – Highest sensitivity

  • True “mix and measure” continuous processing
  • Ultra high sensitivity, 3 times more light output than ATPlite
  • Short equilibrium time for faster processing
  • Signal half-life of 30 minutes

ATPlite – Convenient, extended Signal

  • 2-step format, non-separation assay for time course studies; lysate can be stored frozen and tested later
  • Quantitate from <5 cells/well
  • Prolonged equilibrium time for batch processing
  • Signal half-life of 5 hours
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AlphaLISA immunoassay kits

Heme oxygenase 1 AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human heme oxygenase 1 (HO-1) enables the quantitative determination of human HO-1 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 271.00 GBP - 5940.00 GBP
AlphaLisa Analyte 2

CRP (human), lyophilized (AlphaLISA)

Human C-Reactive Protein (CRP) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human CRP kit, but can be ordered separately.
List Price : 155.00 GBP
Alpha donor antibody coated

Anti-Mouse IgG Alpha Donor beads

Alpha Donor beads conjugated to anti-mouse IgG antibody. This bead can be used to capture mouse IgG antibodies for Alpha no-wash protein detection, antibody detection, antibody binding, and other applications.
Price Range : 302.00 GBP - 5470.00 GBP
AlphaLisa Analyte 2

CXCL1/GROα (human), 0.01 µg, lyophilized (AlphaLISA)

Human CXCL1/GROα standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA Human CXCL1/GROα Detection Kit, but can be ordered separately.
List Price : 155.00 GBP

Anti-Mouse IgG3 (isotyping) AlphaLISA Acceptor beads

AlphaLISA® Acceptor beads conjugated to a anti-mouse IgG3. This bead can be used to create no-wash AlphaLISA assays for isotyping and other applications.
Price Range : 354.00 GBP - 14540.00 GBP

FLT-1/VEGFR 1 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human VEGFR 1 (FLT-1) enables the quantitative determination of VEGFR 1 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 271.00 GBP - 5940.00 GBP

Heme oxygenase 2 AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human heme oxygenase 2 (HO-2) enables the quantitative determination of human HO-2 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 271.00 GBP - 5940.00 GBP
AlphaLISA assay principle

FGF23 (Human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human FGF23 enables the quantitative determination of human FGF23 in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The kit demonstrated no cross reactivity to FGF21 and very low reactivity to Mouse FGF23.
Price Range : 300.00 GBP - 6120.00 GBP
Protein

Glucagon (human), liquid (LANCE)

Human glucagon standard, liquid, for use in running standard curves in LANCE TR-FRET no-wash detection assays. This standard is already provided in the LANCE Ultra Human Glucagon TR-FRET Detection Kit, but can be ordered separately.
List Price : 151.00 GBP

Claudin 7 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human claudin 7 enables the detection and quantitation of claudin 7 in cell culture media and human cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 437.00 GBP - 12290.00 GBP

Glutathione AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to glutathione. These beads can be used to capture GST-tagged proteins and peptides.
Price Range : 369.00 GBP - 15140.00 GBP
Protein

Kappa Light Chain (human), lyophilized (LANCE)

Human kappa light chain standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Human Kappa Light Chain Detection Kit, but can also be ordered separately.
List Price : 169.00 GBP

DELFIA Eu-N1 Anti-Phosphtyrosine (PT66)

Europium labeled anti-phosphotyrosine PT66 antibody for DELFIA® time-resolved fluorescence (TRF) kinase assays. The PT66 antibody is an affinity purified mouse monoclonal antibody that binds to phosphorylated tyrosine residues.
Price Range : 132.00 GBP - 1940.00 GBP

PD-1 (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human PD-1 Detection Kit is designed for detection and quantitation of human PD-1 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 485.00 GBP - 6460.00 GBP
AlphaLisa Analyte 2

MMP9 (mouse), lyophilized (AlphaLISA)

Mouse Matrix Metalloproteinase-9 (MMP9) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA mouse MMP9 kit, but can be ordered separately.
List Price : 332.00 GBP
AlphaLisa Analyte 2

FLT1/VEGFR 1 (human), lyophilized (AlphaLISA)

Human VEGFR 1 (FLT-1) standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human FLT-1 Detection Kit, but can also be ordered separately.
List Price : 146.00 GBP

CXCL1/GRO-α (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human CXCL1/GRO-α Detection Kit is designed for detection and quantitation of human CXCL1 (GRO-α) in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 499.00 GBP - 6630.00 GBP

c-Myc (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra c-Myc Detection Kit is designed for detection and quantitation of c-Myc peptide tag in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 499.00 GBP - 6660.00 GBP

AlphaLISA Anti-methyl Histone H3 Arginine 2 (H3R2me) Acceptor Beads

AlphaLISA® Acceptor beads conjugated to an antibody against human Histone H3 that is methylated at arginine 2 (H3R2me). These beads can be used for AlphaLISA no-wash epigenetic writer and eraser assays.
Price Range : 849.00 GBP - 15450.00 GBP
AlphaPlex-545-antibody-bead2

Anti-Mouse IgG AlphaPlex-545 (Tb) Acceptor Beads

AlphaPlex-545 (Terbium) Acceptor beads conjugated to anti-mouse IgG antibody, for use in Alpha no-wash multiplexing assays.
Price Range : 369.00 GBP - 15130.00 GBP

LC3B (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human LC3B Detection Kit is designed for detection and quantitation of human microtubule-associated protein 1 light chain 3 isoform B (LC3B) in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 499.00 GBP - 6660.00 GBP
AlphaLISA Cellular Epigenetics kits

AlphaLISA Acetylated-Histone H3 Lysine 27 (H3K27ac) Cellular Detection Kit

This kit is designed for the detection of acetylated Histone H3 Lysine 27 (H3K27ac) in cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 415.00 GBP - 7400.00 GBP

PIP Collagen (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra PIP Collagen Detection Kit is designed for detection and quantitation of human PIP collagen in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 485.00 GBP - 6460.00 GBP
AlphaLISA immunoassay kits

IL-6 (mouse) AlphaLISA Detection Kit

The AlphaLISA® Mouse Interleukin-6 (IL-6) Detection Kit is designed for detection and quantitation of mouse IL-6 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 954.00 GBP - 6330.00 GBP

Hyaluronic acid (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human hyaluronic acid enables the detection and quantitation of Human Hyaluronic Acid (hHA) in human serum, plasma, cell lysate, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows no cross-reactivity with human collagen Type I, Type II, Type III, and fibronectin.
Price Range : 436.00 GBP - 6110.00 GBP
AlphaLisa Analyte 2

Heme oxygenase 2, liquid (AlphaLISA)

Human heme oxygenase 2 standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human HO-2 Detection Kit, but can also be ordered separately.
List Price : 146.00 GBP

IL-17A (mouse/rat) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Mouse/Rat IL-17A Detection Kit is designed for detection and quantitation of mouse or rat interleukin 17A in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 499.00 GBP - 6660.00 GBP
britelite plus Reporter Gene Assay kit

britelite plus Reporter Gene Assay System

Our brightest reporter gene assay for quantitation of firefly luciferase in mammalian cells.
Price Range : 55.00 GBP - 3300.00 GBP

PER.C6® HCP AlphaLISA Detection Kit

The AlphaLISA® PER.C6® HCP Detection Kit is designed for the quantitative detection of PER.C6® contaminants in buffer and cell culture media using a homogeneous (no wash steps, no separation steps) assay.
Price Range : 926.00 GBP - 6150.00 GBP
AlphaLisa Analyte 2

IFNγ (bovine), lyophilized (AlphaLISA)

Bovine IFNγ standard, 0.3 µg, for use in AlphaLISA immunoassays. This vial contains enough analyte to run 10 standard curves. This standard is provided in the AlphaLISA bovine IFNγ kits, but can also be ordered separately.
List Price : 210.00 GBP
PerkinElmer

AlphaLISA TruHits Kit

Control beads for identifying false positives and interfering compounds in AlphaLISA® no-wash assays.
Price Range : 358.00 GBP - 2490.00 GBP

Perlecan (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human Perlecan Detection Kit is designed for detection and quantitation of human perlecan in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 499.00 GBP - 6660.00 GBP

MMP-9 (mouse) AlphaLISA Detection Kit

The AlphaLISA® Mouse Matrix Metalloproteinase-9 (MMP9) Detection Kit is designed for detection and quantitation of murine MMP9 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. The kit was designed to detect both MMP9 and pro-MMP9.
Price Range : 1830.00 GBP - 14280.00 GBP
alphaimmunoassay1

G-CSF (human) AlphaLISA Detection Kit

The AlphaLISA® Human Granulocyte Colony-Stimulating Factor (G-CSF) Detection Kit is designed for detection and quantitation of human G-CSF in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.

Price Range : 954.00 GBP - 6330.00 GBP
AlphaLisa Analyte 2

FSH (human), lyophilized (AlphaLISA)

Human follicle stimulating hormone (FSH) standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human FSH Detection Kit, but can also be ordered separately.
List Price : 185.00 GBP

IL-28B (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human IL-28B Detection Kit is designed for detection and quantitation of human IL-28B in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 559.00 GBP - 7450.00 GBP
AlphaLISA Cellular Epigenetics kits

AlphaLISA Acetylated Histone H3 Lysine 9 (H3K9ac) Cellular Detection Kit

This kit is designed for the detection of acetylated Histone H3 Lysine 9 (H3K9ac) in cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 415.00 GBP - 7400.00 GBP

Anti-Mouse IgM (isotyping) AlphaLISA Acceptor beads

AlphaLISA® Acceptor beads conjugated to a anti-mouse IgM. This bead can be used to create no-wash AlphaLISA assays for isotyping and other applications.
Price Range : 354.00 GBP - 14540.00 GBP
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-CREB (Ser133) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-CREB (Ser133) assay is a sandwich immunoassay for quantitative detection of phospho-CREB (phosphorylated on Ser133) in cellular lysates using Alpha Technology.
Price Range : 307.00 GBP - 17790.00 GBP

COL1A1 (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human COL1A1 Detection Kit is designed for detection and quantitation of human COL1A1 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 485.00 GBP - 6460.00 GBP

FSH (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human FSH enables the quantitative determination of human follicle stimulating hormone in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 326.00 GBP - 9770.00 GBP

Anti-Histone H3 (C-term) AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads designed to capture Histone H3 in a homogeneous AlphaLISA assay for DOT1L. Use in conjunction with biotinylated anti-H3K79me2 (AL148).
Price Range : 849.00 GBP - 15450.00 GBP

NGAL (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for NGAL is designed for the quantitative determination of Neutrophil Gelatinase Associated Lipocalin (NGAL) also known as lipocalin 2 in cell culture media and human serum using a homogeneous AlphaLISA assay (no wash steps). The assay shows no cross-reactivity with mouse NGAL, rat NGAL, bovine NGAL and chicken NGAL.
Price Range : 354.00 GBP - 6860.00 GBP
Alpha donor antibody coated

Anti-Rabbit IgG Alpha Donor beads

Alpha Donor beads conjugated to anti-rabbit IgG antibody. This bead can be used to capture rabbit IgG antibodies for Alpha no-wash protein detection, antibody detection, antibody binding, and other applications.
Price Range : 302.00 GBP - 5470.00 GBP

CCL3/MIP1α (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human CCL3/MIP1α Detection Kit is designed for detection and quantitation of human CCL3 (MIP1α) in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 499.00 GBP - 6630.00 GBP
AlphaLISA immunoassay kits

TNFα (mouse) AlphaLISA Biotin-Free Detection Kit

The AlphaLISA Mouse TNFα Biotin-Free Detection Kit is designed for the quantitative detection of mouse TNFα in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).
Price Range : 404.00 GBP - 6310.00 GBP
PerkinElmer

LANCE Ultra m/rIL17A, lyophilized(1.0ug)

LANCE Ultra m/rIL17A, lyophilized(1.0ug)
List Price : 151.00 GBP

DELFIA® Enhancement Solution, 250 mL

DELFIA® Enhancement Solution is used to create a fluorescent Europium or Samarium chelate for measuring DELFIA time-resolved fluorescence (TRF) assays. This solution is required for all DELFIA TRF assays.
List Price : 135.00 GBP
AlphaLisa Analyte 2

IgA (bovine), lyophilized (AlphaLISA)

Bovine IgA standard, 10 µg, for use in AlphaLISA immunoassays. This vial contains enough analyte to run 10 standard curves. This standard is provided in the AlphaLISA bovine IgA kits, but can also be ordered separately.
List Price : 210.00 GBP

DELFIA Eu-Labeling kit (Eu-N1-ITC chelate), 0.2mg

DELFIA® Europium labeling kit for labeling your own biomolecules for use in DELFIA time-resolved fluorescence assays. The ITC chelate reacts with free amine groups in the target biomolecule.
List Price : 323.00 GBP

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