PerkinElmer

Drug Discovery Screening Reagents

OF

Drug discovery is a complex, costly and time-consuming undertaking. For research scientists, screening potential candidates is where the journey begins.

Your job is to move from target to hit to candidate quickly and efficiently – with as little wasted resources as possible. Our drug discovery screening reagents do just that. Whether you are doing high throughput screening or focused screening, we offer a wide range of validated reagents and kits in Alpha chemiluminescent, LANCE® TR-FRET, DELFIA® TRF, and other assay technologies to suit you screening assay needs.

Accelerate your workflow with our drug discovery screening reagents while getting consistent, accurate and physiologically relevant results.

AlphaLISA assay principle
AlphaLISA assay principle

Alpha Technology

Virtually anything can be measured with Alpha Technology quickly and easily, including enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein post-translational modifications, protein:protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous, no-wash assay, with no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerebrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the Alpha solution that’s right for you

  • AlphaLISA® – when running large numbers of ELISA from the same sample or working with difficult matrix, serum or plasma
  • AlphaLISA SureFire® Ultra and AlphaScreen® SureFire – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaPlex – for measuring more than one analyte from the same well, by using the specific wavelengths of Europium, Terbium and Samarium atoms.
  • Alpha SureFire Ultra Multiplex – for measuring two phospho-events or one phospho and a “total” protein control from the same well.
  • AlphaScreen® – perfect for studying fusion-tagged proteins or protein: protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix. Available as AlphaScreen, AlphaLISA and AlphaPlex products.
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf kit, the toolbox gives you everything you need to create your own. Available as AlphaScreen, AlphaLISA and AlphaPlex products.
  • Alpha Biomarker kits – more than 200 validated and ready to use sandwich immunoassays. Available as AlphaLISA and AlphaPlex products.
LANCE biomarker detection assay principle
LANCE biomarker detection assay principle

LANCE Ultra TR-FRET

From assay development to primary and secondary screening, LANCE Ultra Technology provides researchers with robust and easy-to-use assays adaptable to a variety of targets on a broad range of multimode detectors, with the fastest time-to-results in a commercially-available TR-FRET assay.

  • No-wash/separation steps
  • Homogeneous dispense-and-read technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 384-well and 1536-well formats

LANCE and LANCE (LANthanide Chelate Excitation) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One binding partner of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and a second one is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320-340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light detectable at 665 nm.

DELFIA_trf
DELFIA_trf

DELFIA

DELFIA (Dissociation-Enhanced Lanthanide Fluorescence Immunoassay) is a proven, robust immunodetection platform based on time-resolved fluorescence (TRF) detection. This detection method, coupled with the unique chemical properties of lanthanide chelates, makes DELFIA a superior alternative to conventional ELISA for research, drug discovery, and clinical applications.

DELFIA offers a combination of high sensitivity, wide dynamic range and long-lasting stability that traditional ELISA cannot match. 

Reporter gene assay
Reporter gene assay

Reporter Gene Assays

Whether your assay requires the sensitivity provided by high signal intensity, or the flexibility of an extended signal half-life, PerkinElmer provides a solution. Choose from one of three luciferase detection systems: neolite, britelite plus, or steadylite plus depending on the specific requirements of your experiment.
  • No more washing – just add your lites reagent of choice, mix, incubate, and read
  • Hassle-free storage – all lites reagents are designed for extended storage at 2-8 °C (no -20 °C storage required)
  • Odor-friendly, eliminates hood work – lites products do not contain DTT and do not have the hazards and repugnant odor typical of conventional luciferase detection systems
  • Excellent Z’ values – superior S/B ratios allow for excellent Z’ values in antagonist and agonist studies
ATPlite assay principle
ATPlite assay principle

ATP Quantitation

For fast, reliable detection of ATP for cell quantitation, ATPlite 1step and ATPlite are innovative technologies that measure cell proliferation and cytotoxicity in mammalian cells based on the detection of ATP using firefly luciferase.

ATPlite 1step – Highest sensitivity

  • True “mix and measure” continuous processing
  • Ultra high sensitivity, 3 times more light output than ATPlite
  • Short equilibrium time for faster processing
  • Signal half-life of 30 minutes

ATPlite – Convenient, extended Signal

  • 2-step format, non-separation assay for time course studies; lysate can be stored frozen and tested later
  • Quantitate from <5 cells/well
  • Prolonged equilibrium time for batch processing
  • Signal half-life of 5 hours
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"Drug Discovery Screening Reagents" 1-50 of 1424 Products & Services

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AlphaLISA immunoassay kits

CD40 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human CD40 enables the quantitative determination of human cluster of differentiation 40 protein in serum, cell culture media, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 515.00 GBP - 7290.00 GBP
AlphaLISA immunoassay kits

Insulin-like Growth Factor 2 (human) AlphaLISA Detection Kit

The AlphaLISA® Human Insulin-like Growth Factor 2 (IGF2) Detection Kit is designed for detection and quantitation of human IGF2 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1040.00 GBP - 6930.00 GBP
AlphaLisa Analyte 2

CCL1 (human), lyophilized (AlphaLISA)

Recombinant human CCL1 standard, for use in no-wash AlphaLISA quantitation assays. This standard is already included in the AlphaLISA human CCL1 detection kit, but can also be ordered separately.
List Price : 340.00 GBP
Alpha Fc receptor bind

FCGR3A/CD16a (176Val/V158) AlphaLISA Binding Kit

This kit is designed for the detection of binding activity between human FCGR3A (176Val) and human IgG Fc fragment using a homogeneous AlphaLISA® assay (no wash steps). This assay can facilitate the design and development of antibody therapetics by using competitive binding.
Price Range : 477.00 GBP - 7280.00 GBP

Periostin (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human periostin enables the quantitative determination of human periostin in buffer, cell culture media, and serum using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 515.00 GBP - 7290.00 GBP
SureFire principle

AlphaScreen SureFire ALK (p-Tyr1586) Assay Kit

The AlphaScreen® SureFire® p-ALK (Tyr1586) assay is a sandwich immunoassay for quantitative detection of phospho-ALK (phosphorylated on Tyr1586) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 994.00 GBP - 10740.00 GBP

IL-18 (human) AlphaLISA Detection Kit

The AlphaLISA® Human Interleukin 18 (IL-18) Detection Kit is designed for detection and quantitation of human IL-18 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1040.00 GBP - 6930.00 GBP
LANCE Detection Kit

IL-12/p40 (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human IL-12/p40 Detection Kit is designed for detection and quantitation of human interleukin-12/p40 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 547.00 GBP - 7290.00 GBP
AlphaLISA immunoassay kits

Fibrinogen (human) AlphaLISA Detection Kit

The AlphaLISA® Human Fibrinogen Detection Kit is designed for detection and quantitation of Human fibrinogen in human serum, plasma, and cell culture supernatants using a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 306.00 GBP - 6890.00 GBP
AlphaLISA immunoassay kits

TIM-3 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human TIM-3 enables the quantitative determination of human T-cell immunoglobulin and mucin-domain containing-3 (TIM-3) in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 412.00 GBP - 9890.00 GBP

ULight-anti-V5 (LANCE TR-FRET)

Anti-V5 tag antibody labeled with ULight™ dye. This ULight-labeled reagent can be used to capture V5-tagged proteins and peptides, and can be used in conjunction with LANCE® Europium-labeled reagents to create TR-FRET no-wash assays for protein-protein interaction, protein detection, and enzymatic studies.
Price Range : 285.00 GBP - 7210.00 GBP

DELFIA Eu-Labeling kit (Eu-N1-ITC chelate), 0.2mg

DELFIA® Europium labeling kit for labeling your own biomolecules for use in DELFIA time-resolved fluorescence assays. The ITC chelate reacts with free amine groups in the target biomolecule.
List Price : 372.00 GBP

GITR (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human GITR enables the quantitative determination of human glucocorticoid-induced TNFR-related protein (GITR) in serum, cell culture media, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 494.00 GBP - 6990.00 GBP
LANCE Detection Kit

Apo-CIII (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human Apo-CIII Detection Kit is designed for detection and quantitation of ApoC3 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 531.00 GBP - 7070.00 GBP
AL901

AlphaLISA TruHits Biotin-free Detection Kit

Bead control set for identifying false positives and interfering compounds.
Price Range : 1040.00 GBP - 6890.00 GBP
AlphaLisa Analyte 2

Hepatitis B Virus e Antigen (HBeAg), lyophilized (AlphaLISA)

Hepatitis B virus e antigen (HBeAg) standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA HBeAg Detection Kit, but can also be ordered separately.
List Price : 277.00 GBP
AlphaLISA immunoassay kits

IFN-γ (human) AlphaLISA Biotin-Free Detection Kit

The AlphaLISA Human IFNγ Biotin-Free Detection Kit is designed for the quantitative detection of human IFNγ in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).
Price Range : 477.00 GBP - 6900.00 GBP
SureFire principle

AlphaScreen SureFire Phospho-BAD (Ser136) Kit

The AlphaScreen® SureFire® p-BAD (Ser136) assay is a sandwich immunoassay for quantitative detection of phospho-BAD (phosphorylated on Ser136) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 994.00 GBP - 10740.00 GBP
AlphaLISA immunoassay kits

IL-1α (human) AlphaLISA Detection Kit

The AlphaLISA® Human Interleukin 1 alpha (IL1α) Detection Kit is designed for detection and quantitation of human IL1α in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1110.00 GBP - 6930.00 GBP
PerkinElmer

DELFIA® Enhancement Solution, 50 mL

DELFIA® Enhancement Solution is used to create a fluorescent Europium or Samarium chelate for measuring DELFIA time-resolved fluorescence (TRF) assays. This solution is required for all DELFIA TRF assays.
List Price : 31.00 GBP
Protein

PTEN (human), lyophilized (LANCE)

Human PTEN standard, lyophilized, for use in running standard curves in LANCE Ultra TR-FRET detection assays. This standard is already provided in the LANCE Ultra Human PTEN Detection Kit, but can be ordered separately.
List Price : 209.00 GBP

Unconjugated AlphaLISA Acceptor beads

Unconjugated AlphaLISA Acceptor beads are provided so that you can conjugate your biomolecule-of-interest (antibody, protein, peptide, etc.) to create your own AlphaLISA Acceptor beads for use in Alpha no-wash assays.
Price Range : 922.00 GBP - 19380.00 GBP

ULight anti-FITC

ULight™ labeled anti-FITC antibody for LANCE® TR-FRET assays. The anti-FITC antibody is a purified mouse IgG2a monoclonal antibody.
Price Range : 294.00 GBP - 7430.00 GBP

LANCE Ultra Europium-anti-unmodified Histone H3 Lysine 9/Lysine 27 (H3K9/K27) Antibody

Europium-labeled monoclonal antibody recognizing human Histone H3 that is unmodified at lysine 9 and lysine 27 (H3K9/K27). For use in LANCE® Ultra epigenetic writer and eraser assays.
Price Range : 2240.00 GBP - 5610.00 GBP

Lambda light chain (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human lambda light chain enables the quantitative determination of human λ light chain in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 412.00 GBP - 9890.00 GBP

DELFIA® Enhancement Solution, 250 mL

DELFIA® Enhancement Solution is used to create a fluorescent Europium or Samarium chelate for measuring DELFIA time-resolved fluorescence (TRF) assays. This solution is required for all DELFIA TRF assays.
List Price : 155.00 GBP
AlphaLisa Analyte 2

Periostin (human), lyophilized (AlphaLISA)

Human periostin standard, for use in no-wash AlphaLISA quantitation assays. This standard is already included in the AlphaLISA human periostin detection kit, but can also be ordered separately.
List Price : 500.00 GBP

ANGPTL4 (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human ANGPTL4 Detection Kit is designed for detection and quantitation of human angiopoietin-like 4 protein in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 547.00 GBP - 7290.00 GBP
LANCE Detection Kit

GAD67 (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human GAD67 Detection Kit is designed for detection and quantitation of Glutamic acid decarboxylase 67 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 531.00 GBP - 7070.00 GBP
AlphaLISA SureFire Ultra kits

Phospho-ErbB2 (Tyr1221/1222) AlphaLISA SureFire Ultra Detection Kit

The AlphaLISA® SureFire® Ultra™ p-ErbB2 (Tyr1221/1222) assay is a sandwich immunoassay for quantitative detection of phospho-ErbB2 (phosphorylated on Tyr1221/1222) in cellular lysates using Alpha Technology.
Price Range : 326.00 GBP - 18890.00 GBP
AlphaLISA immunoassay kits

CD28 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human CD28 enables the quantitative determination of human CD28 in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 412.00 GBP - 9890.00 GBP
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-AKT1/2/3 (Thr308) Assay Kit

The AlphaLISA® SureFire® Ultra p-AKT1/2/3 (Thr308) assay is a sandwich immunoassay for quantitative detection of phospho-AKT1/2/3 (phosphorylated on Thr308) in cellular lysates using Alpha Technology.
Price Range : 326.00 GBP - 18890.00 GBP

Leptin (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human Leptin Detection Kit is designed for detection and quantitation of human leptin in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 547.00 GBP - 7260.00 GBP

AlphaLISA Biotinylated Anti-Histone H3 (C-ter) Antibody, 200 µg

Biotinylated antibody against the C-terminal end of human histone H3, for use in Alpha no-wash epigenetic assays using full-length histone H3 as substrate.
List Price : 6380.00 GBP
AlphaLISA immunoassay kits

Influenza A Nucleoprotein (NP) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for influenza A nucleoprotein (NP) enables the quantitative determination of nucleoprotein in buffered solution and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 515.00 GBP - 7290.00 GBP
Protein

ANGPTL3 (human), lyophilized (AlphaLISA)

Human Angiopoietin-like 3 (ANGPTL3) standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human ANGPTL3 Detection Kit, but can also be ordered separately.
List Price : 203.00 GBP

Biotin-anti-H3K79me2 antibody

Biotinylated antibody against human histone H3 that is dimethylated on Lysine 79. This antibody is intended for use in AlphaLISA® no-wash epigenetic assays using DOT1L methyltransferase with oligonucleosome substrates.
Price Range : 912.00 GBP - 16590.00 GBP

MMP-23 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human matrix metalloproteinase 23 (MMP-23) enables the quantitative determination of human MMP-23 in buffer, serum, cell culture media, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 494.00 GBP - 7290.00 GBP
AlphaLisa Analyte 2

MMP1 (human), lyophilized (AlphaLISA)

Human Matrix Metalloproteinase-1 (MMP1) standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA human MMP1 kit, but can be ordered separately.
List Price : 169.00 GBP
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-CREB (Ser133) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-CREB (Ser133) in a multiplexed assay.
Price Range : 233.00 GBP - 14030.00 GBP
SureFire principle

AlphaScreen SureFire p53 (p-Ser15) Assay Kit

The AlphaScreen® SureFire® p-p53 (p-Ser15) assay is a sandwich immunoassay for quantitative detection of phospho-p53 (phosphorylated on Ser15) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 994.00 GBP - 10740.00 GBP
LANCE Detection Kit

E cadherin (human) LANCE Ultra TR-FRET Detection Kit

Human E-cadherin standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Human E-cadherin TR-FRET Detection Kit, but can also be ordered separately.
Price Range : 531.00 GBP - 7070.00 GBP

AlphaScreen Phosphotyrosine (PT66) Assay Kit

AlphaScreen phosphoprotein kits are designed to detection phosphoproteins in biochemical or cellular kinase assays. This kit contains streptavidin Donor beads and AlphaScreen Acceptor beads conjugated with the antiphosphotyrosine PT66 antibody. This kit can be used to capture biotinylated phosphoproteins, biotinylated phosphorylated peptides, or biotinylated antibodies in complex with phosphorylated proteins.
Price Range : 156.00 GBP - 9460.00 GBP
AlphaLISA immunoassay kits

CXCL1/GROα (human) AlphaLISA Detection Kit

The AlphaLISA® Human CXCL1/GROα Detection Kit is designed for detection and quantitation of Human CXCL1/GROα in human serum, plasma, and cell culture supernatants using a homogeneous (no-wash steps, no separation steps) assay.
Price Range : 1040.00 GBP - 6890.00 GBP
AlphaLisa Analyte 2

4-1BBL (human), lyophilized (AlphaLISA)

Recombinant human 4-1BBL standard, for use in no-wash AlphaLISA quantitation assays. This standard is already included in the AlphaLISA human 4-1BBL detection kit, but can also be ordered separately.
List Price : 500.00 GBP
AlphaLisa Analyte 2

IFN-λ2 (human), lyophilized (AlphaLISA)

Human interferon lambda-2 (IFN-λ2) standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human IFN-λ2 Detection Kit, but can also be ordered separately.
List Price : 160.00 GBP

ULight anti-c-myc antibody

ULight labeled anti-c-myc antibody for LANCE® TR-FRET assays.
Price Range : 294.00 GBP - 7430.00 GBP

LANCE Ultra Europium-anti-acetyl-Histone H4 Lysine (H4Kac pan) Antibody

Europium-labeled monoclonal antibody recognizing human Histone H4 (H4Kac pan). For use in LANCE® Ultra epigenetic writer and eraser assays.
Price Range : 2240.00 GBP - 5580.00 GBP

Heme oxygenase 1 AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human heme oxygenase 1 (HO-1) enables the quantitative determination of human HO-1 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 297.00 GBP - 6510.00 GBP
AlphaLISA immunoassay kits

CCL18 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human CCL18 enables the quantitative determination of human chemokine (C-C motif) ligand 18 in buffer, human serum, cell media, and PBMC culture supernatant samples using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 515.00 GBP - 7290.00 GBP

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