PerkinElmer

Drug Discovery Screening Reagents

OF

Drug discovery is a complex, costly and time-consuming undertaking. For research scientists, screening potential candidates is where the journey begins.

Your job is to move from target to hit to candidate quickly and efficiently – with as little wasted resources as possible. Our drug discovery screening reagents do just that. Whether you are doing high throughput screening or focused screening, we offer a wide range of validated reagents and kits in Alpha chemiluminescent, LANCE® TR-FRET, DELFIA® TRF, and other assay technologies to suit you screening assay needs.

Accelerate your workflow with our drug discovery screening reagents while getting consistent, accurate and physiologically relevant results.

AlphaLISA assay principle
AlphaLISA assay principle

Alpha Technology

Virtually anything can be measured with Alpha Technology quickly and easily, including enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein post-translational modifications, protein:protein interactions, peptides, sugars and small molecules.

  • Ease-of-use: homogeneous, no-wash assay, with no separation steps
  • Fast: results in 3 hours for most applications
  • High sensitivity: detect down to femtogram levels of target
  • Large dynamic range: 4-5 logs
  • Compatible with many samples types: serum, plasma, cerebrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the Alpha solution that’s right for you

  • AlphaLISA® – when running large numbers of ELISA from the same sample or working with difficult matrix, serum or plasma
  • AlphaLISA SureFire® Ultra and AlphaScreen® SureFire – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
  • AlphaPlex – for measuring more than one analyte from the same well, by using the specific wavelengths of Europium, Terbium and Samarium atoms.
  • Alpha SureFire Ultra Multiplex – for measuring two phospho-events or one phospho and a “total” protein control from the same well.
  • AlphaScreen® – perfect for studying fusion-tagged proteins or protein: protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix. Available as AlphaScreen, AlphaLISA and AlphaPlex products.
  • Alpha Toolbox – when your target doesn’t have an off-the-shelf kit, the toolbox gives you everything you need to create your own. Available as AlphaScreen, AlphaLISA and AlphaPlex products.
  • Alpha Biomarker kits – more than 200 validated and ready to use sandwich immunoassays. Available as AlphaLISA and AlphaPlex products.
LANCE biomarker detection assay principle
LANCE biomarker detection assay principle

LANCE Ultra TR-FRET

From assay development to primary and secondary screening, LANCE Ultra Technology provides researchers with robust and easy-to-use assays adaptable to a variety of targets on a broad range of multimode detectors, with the fastest time-to-results in a commercially-available TR-FRET assay.

  • No-wash/separation steps
  • Homogeneous dispense-and-read technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 384-well and 1536-well formats

LANCE and LANCE (LANthanide Chelate Excitation) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One binding partner of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and a second one is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320-340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light detectable at 665 nm.

DELFIA_trf
DELFIA_trf

DELFIA

DELFIA (Dissociation-Enhanced Lanthanide Fluorescence Immunoassay) is a proven, robust immunodetection platform based on time-resolved fluorescence (TRF) detection. This detection method, coupled with the unique chemical properties of lanthanide chelates, makes DELFIA a superior alternative to conventional ELISA for research, drug discovery, and clinical applications.

DELFIA offers a combination of high sensitivity, wide dynamic range and long-lasting stability that traditional ELISA cannot match. 

Reporter gene assay
Reporter gene assay

Reporter Gene Assays

Whether your assay requires the sensitivity provided by high signal intensity, or the flexibility of an extended signal half-life, PerkinElmer provides a solution. Choose from one of three luciferase detection systems: neolite, britelite plus, or steadylite plus depending on the specific requirements of your experiment.
  • No more washing – just add your lites reagent of choice, mix, incubate, and read
  • Hassle-free storage – all lites reagents are designed for extended storage at 2-8 °C (no -20 °C storage required)
  • Odor-friendly, eliminates hood work – lites products do not contain DTT and do not have the hazards and repugnant odor typical of conventional luciferase detection systems
  • Excellent Z’ values – superior S/B ratios allow for excellent Z’ values in antagonist and agonist studies
ATPlite assay principle
ATPlite assay principle

ATP Quantitation

For fast, reliable detection of ATP for cell quantitation, ATPlite 1step and ATPlite are innovative technologies that measure cell proliferation and cytotoxicity in mammalian cells based on the detection of ATP using firefly luciferase.

ATPlite 1step – Highest sensitivity

  • True “mix and measure” continuous processing
  • Ultra high sensitivity, 3 times more light output than ATPlite
  • Short equilibrium time for faster processing
  • Signal half-life of 30 minutes

ATPlite – Convenient, extended Signal

  • 2-step format, non-separation assay for time course studies; lysate can be stored frozen and tested later
  • Quantitate from <5 cells/well
  • Prolonged equilibrium time for batch processing
  • Signal half-life of 5 hours
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"Drug Discovery Screening Reagents" 1-50 of 1359 Products and Services

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AlphaLisa Analyte 2

IL-5 (mouse), lyophilized (AlphaLISA)

Mouse IL-5 standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA mouse IL-5 Detection Kit, but can also be ordered separately.
List Price : 191.00 GBP
AlphaLisa Analyte 2

IL-25 (mouse), liquid (AlphaLISA)

Mouse IL-25 standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA mouse IL-25 Detection Kit, but can also be ordered separately.
List Price : 191.00 GBP
AlphaLISA immunoassay kits

IL-5 (mouse) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for mouse IL-5 enables the quantitative determination of mouse interleukin-5 in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 388.00 GBP - 9300.00 GBP

LANCE Ultra Europium-anti-methyl-Histone H3/H4 Arginine (H3/H4Rme pan) Antibody

Europium-labeled monoclonal antibody recognizing human Histone H3 and H4 methylated arginine residues. For use in LANCE® Ultra epigenetic writer and eraser assays.
Price Range : 2140.00 GBP - 5350.00 GBP
SureFire principle

AlphaScreen SureFire SMAD2 (p-Ser465;Ser467) Assay Kit

The AlphaScreen® SureFire® p-SMAD2 (p-Ser465/467) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD2 (phosphorylated on Ser465/467) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 965.00 GBP - 10430.00 GBP
AlphaLISA immunoassay kits

IL-13 (bovine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for bovine IL-13 enables the quantitative determination of bovine interleukin-13 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 280.00 GBP - 6120.00 GBP
AlphaLISA Buffer

AlphaLISA universal buffer

Pre-formulated buffer used to QC Alpha assays. This buffer can sometimes be used for protein-protein binding, proof-of-concept, and other Alpha assays.
Price Range : 130.00 GBP - 248.00 GBP

AlphaScreen PhosphoSensor Kit

Antibody-free assay for kinase activity via dectection of phosphorylated protein or peptide substrate.
Price Range : 248.00 GBP - 1860.00 GBP
AlphaLISA immunoassay kits

IL-17A (porcine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for porcine interleukin 17A (IL-17A) enables the quantitative determination of porcine IL-17A in serum and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 688.00 GBP - 6660.00 GBP
LANCE Detection Kit

PD-L1 (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human PD-L1 Detection Kit is designed for detection and quantitation of human PD-L1 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 499.00 GBP - 6660.00 GBP
AlphaLisa Analyte 2

TSLP (mouse), lyophilized (AlphaLISA)

Mouse Thymic stromal lymphopoietin (TSLP) standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA mouse TSLP Detection Kit, but can also be ordered separately.
List Price : 191.00 GBP
Protein

IL-31 (Human) lyophilized (AlphaLISA)

Human IL-31 standard, lyophilized, for use in running standard curves in AlphaLISA no-wash immunoassays. This standard is already provided in the AlphaLISA IL-31 Detection Kit, but can be ordered separately.
List Price : 197.00 GBP

LANCE Ultra Europium-anti-methyl-Histone H3 Lysine 9 (H3K9me2)

Europium-labeled monoclonal antibody recognizing human Histone H3 di-methylated at lysine 9 (H3K9me2). For use in LANCE® Ultra epigenetic writer and eraser assays.
Price Range : 2140.00 GBP - 5360.00 GBP

BCL-2 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for BCL-2 enables the detection and quantitation of human BCL-2 in serum, buffered solution, or cell culture medium using a homogeneous AlphaLISA assay (no wash steps). This kit is not cross reactive with mouse Bcl-2.
Price Range : 402.00 GBP - 13070.00 GBP
AlphaPlex Terbium immunoassay kits

AlphaPlex-545 Human IL1β Detection Kit

The AlphaPlex™ 545 Human IL1β Detection Kit is designed for the quantitative determination of human IL1β together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous assay.
Price Range : 380.00 GBP - 6490.00 GBP
DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG

DELFIA® Eu-N1 Anti-HA

Europium-labeled anti-HA antibody for binding HA-tagged proteins in DELFIA® time-resolved fluorescence (TRF) assays. The anti-HA antibody is a purified IgG2b subclass of mouse monoclonal antibody. It recognizes the epitope sequence (YPYDVPDYA) derived from the human influenza hemagglutinin (HA) protein.
Price Range : 387.00 GBP - 3680.00 GBP

C-peptide (mouse/rat) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Mouse C-peptide Detection Kit is designed for detection and quantitation of Mouse C-peptide in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 514.00 GBP - 6830.00 GBP
AlphaLisa Analyte 2

PIGF (human), lyophilized (AlphaLISA)

Human placental growth factor (PIGF) standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA human PIGF Detection Kit, but can also be ordered separately.
List Price : 151.00 GBP
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-IGF Receptor β (Tyr1135/1136) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-IGF Receptor β (Tyr1135/1136) in a multiplexed assay.
Price Range : 226.00 GBP - 13610.00 GBP
AlphaLisa Analyte 2

LDHA (human), liquid (AlphaLISA)

Human lactate dehydrogenase A subunit recombinant protein standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA Human LDHA Detection Kit, but can also be ordered separately.
List Price : 241.00 GBP
AlphaLisa Analyte 2

IL-6 (porcine), lyophilized (AlphaLISA)

Porcine interleukin 6 (IL-6) standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA Porcine IL-6 Detection Kit, but can also be ordered separately.
List Price : 456.00 GBP
AlphaLISA immunoassay kits

ANGPTL4 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human ANGPTL4 enables the quantitative determination of ANGPTL4 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 280.00 GBP - 6120.00 GBP
LANCE Detection Kit

VEGF (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human VEGF Detection Kit is designed for detection and quantitation of human VEGF in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 514.00 GBP - 6860.00 GBP

IL-6 (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra human IL-6 Detection Kit is designed for detection and quantitation of human interleukin-6 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 514.00 GBP - 6830.00 GBP

AlphaLISA Anti-acetyl-p53 Lysine 382 (p53K382ac) Acceptor Beads

AlphaLISA® Acceptor beads conjugated to an antibody against human p53 that is acetylated at lysine 382 (p53K382ac). These beads can be used for no-wash AlphaLISA epigenetic writer and eraser assays.
Price Range : 874.00 GBP - 15910.00 GBP
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-VEGF Receptor 2 (Tyr1175) Assay Kit

The AlphaLISA® SureFire® Ultrap-VEGF Receptor 2 (Tyr1175) assay is a sandwich immunoassay for quantitative detection of phospho-VEGFR Receptor 2 (phosphorylated on Tyr1175) in cellular lysates using Alpha Technology.
Price Range : 316.00 GBP - 18330.00 GBP

LANCE Ultra cAMP Detection Kit

The LANCE® Ultra cAMP assay is a homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) immunoassay designed to measure cAMP produced upon modulation of adenylyl cyclase activity by G-protein coupled receptors (GPCRs).
Price Range : 392.00 GBP - 5090.00 GBP
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular detection assay

Total ERK 1/2 LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra Total ERK 1/2 kits are designed for the detection of total (phosphorylated and non-phosphorylated) ERK1 and ERK2 in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay can be used as a normalization assay for phospho-ERK detection, and is compatible with both adherent and suspension cells.
Price Range : 892.00 GBP - 4000.00 GBP
alphascreen surefire total

AlphaScreen SureFire Total Akt1 research kit

The AlphaScreen® SureFire® Akt 1 (total) assay is a sandwich immunoassay for quantitative detection of Akt 1 in cellular lysates using Alpha Technology. The assay is an ideal system for normalizing Akt levels for experiments measuring changes in Akt phosphorylation. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1070.00 GBP - 11430.00 GBP

Total Ghrelin (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human total ghrelin enables the quantitative determination of total ghrelin in serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 388.00 GBP - 11630.00 GBP
AlphaLISA immunoassay kits

CXCL10/IP-10 (mouse) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for mouse Interferon Gamma Induced Protein 10 (mIP-10)/CXCL10 enables the quantitative determination of mouse IP-10 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 280.00 GBP - 6120.00 GBP
AlphaLISA immunoassay kits

Chloramphenicol AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for Chloramphenicol enables the quantitative determination of Chloramphenicol in buffered solution, sample extracts, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 276.00 GBP - 6510.00 GBP
PerkinElmer

LANCE Eu-W1024 Protein G

Europium-labeled Protein G, for capturing antibodies in LANCE® TR-FRET assays.
Price Range : 164.00 GBP - 5620.00 GBP

Protein A AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to Protein A. These beads can be used to capture antibodies that associate with Protein A.
Price Range : 380.00 GBP - 15590.00 GBP
LANCE Detection Kit

Leptin (mouse) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Mouse Leptin Detection Kit is designed for detection and quantitation of mouse leptin in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 514.00 GBP - 6860.00 GBP

Protein G AlphaLISA Acceptor Beads

AlphaLISA® Acceptor beads conjugated to Protein G. These beads can be used to capture antibodies that associate with Protein G.
Price Range : 380.00 GBP - 15590.00 GBP
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-STAT5 (Tyr694/699) Assay Kit

The AlphaLISA® SureFire® Ultra p-STAT5 (Tyr694/699) HV (high volume) assay is a sandwich immunoassay for quantitative detection of phospho-STAT5 (phosphorylated on Tyr694/699) in cellular lysates using Alpha Technology. This kit replaces ALSU-PST5-A-HV. ALSU-PST5-A-HV is no longer available as the antibody(ies) have been discontinued by the supplier. A new antibody pair has been selected and validated to make this new version, with a similar or slightly improved performance over the initial version. Comparative data are included in the manuals of the new kits.
Price Range : 316.00 GBP - 18330.00 GBP

LANCE Ultra ULight™-DNA Topoisomerase 2-alpha (Thr1342) Peptide

Synthetic peptide containing residues surrounding Thr1342 of human DNA Topoisomerase 2-alpha; phosphorylation site: Thr1342. The ULight-DNA Topoisomerase 2-alpha (Thr1342) peptide can be phosphorylated by PLK3, CK2α and other kinases. Phosphorylation motif: DEKTDDE
Price Range : 241.00 GBP - 531.00 GBP
AlphaLISA immunoassay kits

PON-2 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human PON-2 enables the quantitative determination of human Paraoxonase 2 (PON-2) in buffered solution, cell culture media, and cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 470.00 GBP - 9030.00 GBP

PIGF (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human placental growth factor (PIGF) enables the quantitative determination of human PIGF (PGF) in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 280.00 GBP - 6120.00 GBP
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-mTOR (Ser2448) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-mTOR (Ser2448) in a multiplexed assay.
Price Range : 226.00 GBP - 13610.00 GBP
AlphaLisa Analyte 2

IL-4 (mouse), lyophilized (AlphaLISA)

Mouse IL-4 standard, for use in running standard curves in AlphaLISA assays. This standard is already provided in the AlphaLISA mouse IL-4 Detection Kit, but can also be ordered separately.
List Price : 191.00 GBP
LANCE Detection Kit

IL-1RA (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human IL-1RA Detection Kit is designed for detection and quantitation of human interleukin-1RA in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 576.00 GBP - 7680.00 GBP
AlphaLisa Analyte 2

IL-31 (human), lyophilized (LANCE)

Human IL-31 standard, for use in running standard curves in LANCE Ultra TR-FRET assays. This standard is already provided in the LANCE Ultra Human IL-31 TR-FRET Detection Kit, but can also be ordered separately.
List Price : 151.00 GBP

TNFR1 (human, soluble) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for soluble human tumor necrosis factor receptor 1 (TNFR1) enables the quantitative determination of soluble human TNFR1 in buffered solution, serum, and cell culture media using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 485.00 GBP - 6860.00 GBP

TDP-43 (human) LANCE Ultra TR-FRET Detection Kit

The LANCE® Ultra Human TDP-43 Detection Kit is designed for detection and quantitation of human TDP-43 in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
Price Range : 499.00 GBP - 6660.00 GBP

TIM-3 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human TIM-3 enables the quantitative determination of human T-cell immunoglobulin and mucin-domain containing-3 (TIM-3) in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 388.00 GBP - 9300.00 GBP

IL-23 (human) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for human IL-23 enables the quantitative determination of human interleukin-23 in human serum, plasma, cell lysates, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 399.00 GBP - 11980.00 GBP
AlphaLISA Cellular Epigenetics kits

AlphaLISA dimethylated-Histone H3 Lysine 36 (H3K36me2) Cellular Detection Kit

This kit is designed for the detection of di-methylated Histone H3 Lysine 36 (H3K36me2) in cell lysates using a homogeneous AlphaLISA assay (no wash steps).
Price Range : 428.00 GBP - 7620.00 GBP

IL-8 (bovine/ovine) AlphaLISA Detection Kit

The AlphaLISA® immunoassay kit for bovine IL-8 enables the detection and quantitation of bovine IL8 (bIL8) in serum, buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). This kit is 100% cross-reactive with Ovine IL-8.
Price Range : 449.00 GBP - 6490.00 GBP

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