Enzymology

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The AlphaLISA^® SureFire^® Ultra^™p-VEGF Receptor 2 (Tyr1175) assay is a sandwich immunoassay for quantitative detection of phospho-VEGFR Receptor 2 (phosphorylated on Tyr1175) in cellular lysates using Alpha Technology.

Europium-labeled rabbit polyclonal antibody recognizing human Muscle Glycogen Synthase phosphorylated at Ser641. Antibody labeled with the LANCE^® Eu-W1024-ITC chelate. Sequence motif recognized: PApSVPPSPSL

Antibody-free assay for kinase activity via dectection of phosphorylated protein or peptide substrate.

The AlphaScreen^® SureFire^® p-IKKα (Ser176/180) assay is a sandwich immunoassay for quantitative detection of phospho-IKKα (phosphorylated on Ser176/180) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

Europium-labeled rabbit monoclonal antibody recognizing human 40S ribosomal protein S6 phosphorylated at both Ser235 and Ser236. Sequence motif recognized: RRL(pS)(pS)LRA

The AlphaLISA® SureFire® Ultra™ p-Insulin Receptor β (Tyr1150/1151) assay is a sandwich immunoassay for quantitative detection of phospho-INSR (phosphorylated on Tyr1150/1151) in cellular lysates using Alpha Technology.

The AlphaScreen^® SureFire^® p-PDK1 (p-Ser241) assay is a sandwich immunoassay for quantitative detection of phospho-PDK1 (phosphorylated on Ser241) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

cGMP radioimmunoassay kit using Scintillation Proximity Assay (SPA) beads

The AlphaLISA® SureFire® Ultra™ Total ERK 1/2 assay is a sandwich immunoassay for quantitative detection of ERK 1/2 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.

The AlphaScreen^® SureFire^® p-ATF2 (Thr71) assay is a sandwich immunoassay for quantitative detection of phospho-ATF2 (phosphorylated on Thr71) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

Mouse monoclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects human p70 S6K phosphorylated at Thr389. p70 S6K is phosphorylated in vivo by mTOR. p70 S6K peptides are also phosphorylated by several kinases including COT, HGK, IKKε, IRAK1, IRAK4, MAP4K2, MINK, MST1, MST2, NEK1, NEK2, NEK6, NEK7, PEK, PLK1 and TAOK2. Sequence motif recognized: LGFTYVAP

The AlphaLISA® SureFire® Ultra™ p-4E-BP1 (Thr37/46) assay is a sandwich immunoassay for quantitative detection of phospho-4EBP1 (phosphorylated on Thr37/46) in cellular lysates using Alpha Technology.

AlphaPlex^™-545 (Terbium) Acceptor beads conjugated to antibody against human Histone H3 that is unmodified (H3K4), for use in Alpha no-wash multiplexing epigenetic assays.

Mouse monoclonal antibody labeled with the LANCE® Eu-W1024-ITC chelate. This antibody recognizes specifically phosphorylated Thr232 in myelin basic protein (MBP; Swiss-Prot: P02686). MBP can be phosphorylated by a variety of kinases including Erk1/Erk2. Sequence motif recognized: FFKNIVTPRpTPPPSQGK

The AlphaScreen^® SureFire^® p-GSK 3α (Ser21) assay is a sandwich immunoassay for quantitative detection of phospho-GSK3α (phosphorylated on Ser21) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

LANCE Ultra phospho-EGFR (Tyr1068) kits are designed for the detection of EGFR (phosphorylated at residue Y1068) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.

The AlphaScreen^® SureFire^® p-Caspase 9 (Ser196) assay is a sandwich immunoassay for quantitative detection of phospho-Caspase 9 (phosphorylated on Ser196) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

The AlphaScreen^® SureFire^® p-c-Jun (Ser73) assay is a sandwich immunoassay for quantitative detection of phospho-c-Jun (phosphorylated on Ser73) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

The AlphaScreen^® SureFire^® p-Akt1 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-Akt1 (phosphorylated on Ser473) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

The AlphaLISA^® SureFire^® Ultra^™ p-STAT5 (Tyr694/699) HV (high volume) assay is a sandwich immunoassay for quantitative detection of phospho-STAT5 (phosphorylated on Tyr694/699) in cellular lysates using Alpha Technology. This kit replaces ALSU-PST5-A-HV. ALSU-PST5-A-HV is no longer available as the antibody(ies) have been discontinued by the supplier. A new antibody pair has been selected and validated to make this new version, with a similar or slightly improved performance over the initial version. Comparative data are included in the manuals of the new kits.

The AlphaScreen^® SureFire^® p-ATF1 (Ser63) assay is a sandwich immunoassay for quantitative detection of phospho-ATF1 (phosphorylated on Ser63) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

LANCE® Ultra TR-FRET Ser/Thr kinase substrate selection kit, containing five different ULight-peptide substrates and corresponding Europium anti-phospho antibodies for kinase reactions.

AlphaLISA® Acceptor beads conjugated to an antibody against human histone H4 methylated at arginine 3 (H4R3me). This bead can be used to create AlphaLISA no-wash epigenetic writer and eraser assays.

ULight™-labeled synthetic peptide derived from residues 979-989 of mouse Insulin receptor substrate 1 (IRS-1). The ULight-IRS-1 peptide can be phosphorylated at Tyr983 by INSR and JAK2 kinases. Peptide sequence : CKKSRGDYMTMQIG

The AlphaLISA® SureFire® Ultra™ Total STAT3 assay is a sandwich immunoassay for quantitative detection of STAT3 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.

The AlphaLISA® SureFire® Ultra™ p-IKKα (Ser176/180) assay is a sandwich immunoassay for quantitative detection of phospho-IKKα (phosphorylated on Ser176/180) in cellular lysates using Alpha Technology.

Fluorescently-labelled peptide substrate 5 for kinase assays (inhibiton, target discovery, lead optimization)

ULight™-labeled synthetic peptide containing the amino acid residues surrounding Thr389 of human p70 S6K. The ULight-p70 S6K peptide can be phosphorylated at Thr389 by several kinases including mTOR, COT, HGK, IKKε, IRAK1, IRAK4, MAP4K2, MINK, MST1, MST2, NEK1, NEK2, NEK6, NEK7, PEK, PLK1, and TAOK2. Phosphorylation motif: LGFTYVAP

AlphaLISA® Acceptor beads conjugated to an antibody against human histone H3 mono- or di-methylated at lysine 4 (H3K4me1-2). These beads can be used for no-wash AlphaLISA epigenetic writer and eraser assays.

The AlphaScreen^® SureFire^® p-NF-κB p65 (p-Ser536) assay is a sandwich immunoassay for quantitative detection of phospho-NF-κB p65 (phosphorylated on Ser536) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

The AlphaScreen^® SureFire^® p-CHK1 (Ser345) assay is a sandwich immunoassay for quantitative detection of phospho-CHK1 (phosphorylated on Ser345 in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

Europium-labeled monoclonal antibody recognizing human Histone H4 (H4Kac pan). For use in LANCE® Ultra epigenetic writer and eraser assays.

The AlphaScreen^® SureFire^® p-Cofilin (Ser3) assay is a sandwich immunoassay for quantitative detection of phospho-Cofilin (phosphorylated on Ser3) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

The AlphaLISA^® SureFire^® Ultra^™ p-STAT6 (Tyr641) assay is a sandwich immunoassay for quantitative detection of phospho-STAT6 (phosphorylated on Tyr641) in cellular lysates using Alpha Technology.

The AlphaScreen^® SureFire^® p-ALK (Tyr1604) assay is a sandwich immunoassay for quantitative detection of phospho-ALK (phosphorylated on Tyr1604) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

The AlphaScreen^® SureFire^® p-CHK2 (Thr68) assay is a sandwich immunoassay for quantitative detection of phospho-CHK2 (phosphorylated on Thr68 in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

AlphaLISA® Acceptor beads designed to capture Histone H3 in a homogeneous AlphaLISA assay for DOT1L. Use in conjunction with biotinylated anti-H3K79me2 (AL148).

AlphaLISA no-wash assay kit for the detection of total human Histone 2A (H2A) in cell extracts or cells in culture medium.

The AlphaScreen^® SureFire^® p-Akt1/2/3 (Thr308) assay is a sandwich immunoassay for quantitative detection of phospho-Akt1/2/3 (phosphorylated on Thr308) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.

AlphaLISA® Acceptor beads conjugated to an antibody against human histone H3 mono- or di-methylated at lysine 27 (H3K27me2-1). These beads can be used for no-wash AlphaLISA epigenetic writer and eraser assays.

The AlphaLISA® SureFire® Ultra™ Total Akt1 assay is a sandwich immunoassay for quantitative detection of Akt1 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.

AlphaLISA® biotinylated monoclonal antibody recognizing the amino-terminal end of human histone H4. Broad species cross-reactivity is expected based on sequence similarity. This product is designed to be used with AlphaLISA® Epigenetics Acceptor beads to detect modified full-length histone H4 in homogeneous AlphaLISA assays.

The AlphaLISA^® SureFire^® Ultra^™ Total mTOR assay is a sandwich immunoassay for quantitative detection of mTOR (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.

The AlphaLISA^® SureFire^® Ultra^™ p-SMAD1 (Ser463/Ser465) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD1 (phosphorylated on Ser463/Ser465) in cellular lysates using Alpha Technology.

PKC substrate (sequence: CRFARKGSLRQKNV) labeled with ULight™ dye. Synthetic peptide derived from amino acids 19-31 of PKC?.

LANCE Ultra phospho-AKT1 (Ser473) kits are designed for the detection of AKT1 (phosphorylated at residue S473) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.

Fluorescently-labelled peptide substrate 8 for kinase assays (inhibiton, target discovery, lead optimization)

Fluorescently-labelled peptide substrate 6 for kinase assays (inhibiton, target discovery, lead optimization)

Europium-labeled monoclonal antibody recognizing human Histone H3 and H4 methylated arginine residues. For use in LANCE® Ultra epigenetic writer and eraser assays.