PerkinElmer

Kinase

OF

Study phosphorylation of protein, peptide, and lipid substrates for kinase inhibition, screening, and mechanism-of-action studies. We offer a range of end-point and real-time kinetic assay kits, technologies, and instruments such as:

  • Labeled substrates for kinetic assays and antibody-free screening
  • Alpha kits for highly-sensitive detection of cellular phosphorylation
  • Fast and simple TR-FRET assays using pre-validated peptide and protein substrates derived from biologically-relevant targets
  • Microfluidic mobility-shift assays (MSA) for ratiometric analysis of substrate conversion
  • Multi-mode plate readers for maximal speed and throughput independent of technology platform

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Antibody Conjugates
  • Anti-P-Ser 14-3-3 (1)
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  • Anti-phospho-4EBP1 (1)
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  • Kinase (138)
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  • Protein Analysis & Detection (97)
Assay points(200-50,000)
  • 100 (32)
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Assay Target
  • 4EBP1 (3)
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  • Akt1/2 (1)
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  • STAT6 (1)
  • SYK (2)
  • Topo IIα (2)
  • VEGF2 Receptor (2)
  • β-Catenin (1)
Assay Target Class
  • Phosphoprotein (114)
  • Protein (14)
Automation Compatible
  • Yes (184)
Buffer
  • Aqueous (1)
  • Tricine (7)
  • Tricine with stabilizer and dye (6)
Concentration
  • 10 mCi/mL (8)
  • 12.5 mCi/mL (3)
  • 2 mCi/mL (1)
  • 5 mCi/mL (2)
Detection Method
  • Absorbance (1)
  • Alpha (133)
  • DELFIA TRF (5)
  • Fluorescence (1)
  • Image-based Cytometry (1)
  • Label-free (1)
  • Luminescence (1)
  • Radiometric (16)
  • Time-Resolved Fluorescence (TRF) (48)
  • TR-FRET (42)
Experimental Type
  • In vitro (158)
Fluorophore
  • 5-FAM (29)
  • Eu-N1 (5)
  • Eu-W1024 (22)
  • SureLight-APC (1)
  • ULight (19)
Format
  • Microplates (173)
Label Position
  • alpha (1)
  • gamma (15)
Maximum Temperature (Celsius)
  • 40-50 (2)
  • 50-60 (2)
Molecular Modification
  • Phosphorylation (118)
Nucleotide
  • ATP (12)
  • GTP (4)
Product Brand Name
  • Alpha SureFire Ultra Multiplex (8)
  • AlphaLISA SureFire Ultra (51)
  • AlphaPlex (8)
  • AlphaScreen (4)
  • AlphaScreen SureFire (68)
  • DELFIA (5)
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  • EnVision (2)
  • Labchip EZ Reader (42)
  • LANCE (4)
  • LANCE SureLight (1)
  • LANCE Ultra (37)
  • NEN (2)
  • Victor (2)
Radionuclide
  • 32P (9)
  • 33P (2)
  • 35S (5)
Radioisotope
  • P-32 (9)
  • P-33 (2)
  • S-35 (5)
Sample Volume
  • 2 mL (1)
  • 35 mL (1)
  • 400 mL (1)
Target Species
  • Human (107)
Volume
  • 0-50 (2)
  • 351-400 (1)

"Kinase" 1-50 of 251 Products and Services

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EnSight multimode plate-reader

EnSight Multimode Plate Reader

The EnSight multimode plate reader combines labeled, label-free* & image-based cytometry technologies in a single benchtop platform. Imaging & online data analysis is fast: a 384-well plate can be imaged in less than 5 mins. The system’s modular design, combined with workflow-based Kaleido data acquisition & analysis software, gives a truly versatile plate reader that gets users productive quickly.

*Label free detection module of EnSight Plate Reader no longer available in EU. Please contact your local representative for further details.

AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-ERK1/2 (Thr202/Tyr204) Assay Kit

The AlphaLISA® SureFire® Ultra p-ERK 1/2 (Thr202/Tyr204) assay is a sandwich immunoassay for quantitative detection of phospho-ERK 1/2 (phosphorylated on Thr202/Tyr204 in ERK1, or Thr185/Tyr187 in ERK2) in cellular lysates using Alpha Technology.
Price Range : 307.00 GBP - 17790.00 GBP
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra Total GAPDH (Human/Mouse) Assay Kit

The AlphaLISA® SureFire® Ultra Mouse/Human GAPDH assay is a sandwich immunoassay for quantitative detection of human or mouse GAPDH in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization assay.
Price Range : 298.00 GBP - 17300.00 GBP
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-STAT5 (Tyr694/699) Assay Kit

The AlphaLISA® SureFire® Ultra p-STAT5 (Tyr694/699) HV (high volume) assay is a sandwich immunoassay for quantitative detection of phospho-STAT5 (phosphorylated on Tyr694/699) in cellular lysates using Alpha Technology. This kit replaces ALSU-PST5-A-HV. ALSU-PST5-A-HV is no longer available as the antibody(ies) have been discontinued by the supplier. A new antibody pair has been selected and validated to make this new version, with a similar or slightly improved performance over the initial version. Comparative data are included in the manuals of the new kits.
Price Range : 307.00 GBP - 17820.00 GBP
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-Ribosomal Protein S6 (Ser240/244) Assay Kit, 10,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-rpS6 (Ser240/244) assay is a sandwich immunoassay for quantitative detection of phospho-Ribosomal Protein S6 (phosphorylated on Ser240/244) in cellular lysates using Alpha no-wash technology.
List Price : 5740.00 GBP
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-Ribosomal Protein S6 (Ser240/244) Assay Kit, 500 Assay Points

The AlphaLISA® SureFire® Ultra™ p-rpS6 (Ser240/244) assay is a sandwich immunoassay for quantitative detection of phospho-Ribosomal Protein S6 (phosphorylated on Ser240/244) in cellular lysates using Alpha no-wash technology.
List Price : 1040.00 GBP
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-Ribosomal Protein S6 (Ser240/244) Assay Kit, 50,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-rpS6 (Ser240/244) assay is a sandwich immunoassay for quantitative detection of phospho-Ribosomal Protein S6 (phosphorylated on Ser240/244) in cellular lysates using Alpha no-wash technology.
List Price : 17820.00 GBP
SureFire principle

AlphaScreen SureFire 4EBP1 (p-Thr70) Assay Kit

The AlphaScreen® SureFire® p-4EBP1 (Thr70) assay is a sandwich immunoassay for quantitative detection of phospho-4EBP1 (phosphorylated on Thr70) in cellular lysates using Alpha Technology.
Price Range : 936.00 GBP - 10110.00 GBP
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-NFκB (Ser536) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-NFκB (Ser536) assay is a sandwich immunoassay for quantitative detection of phospho-NFκB (phosphorylated on Ser536) in cellular lysates using Alpha Technology.
Price Range : 307.00 GBP - 5720.00 GBP
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-MEK1 (Ser218/222) Assay Kit, 10,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-MEK1 (Ser218/222) assay is a sandwich immunoassay for quantitative detection of phospho-MEK1 (phosphorylated on Ser218/222) in cellular lysates using Alpha no-wash technology.
List Price : 5740.00 GBP

Guanosine 5`-(γ-thio)triphosphate, [35S]-, 10mM Tricine (pH 7.6), 10mM DTT, 1.0mC/mL

Guanosine triphosphate, labeled on the gamma phosphate group with 35S (GTPγS).
Price Range : 680.00 GBP - 1190.00 GBP

LANCE Ultra ULight™-Crosstide

Crosstide peptide (sequence: CGSGSGRPRTSSFAEG) labeled with ULight™ dye. Synthetic peptide derived from glycogen synthase kinase-3 alpha (GSK-3α) (Phosphorylation site: Ser21). GSK-3α can be phosphorylated by a variety of kinases including AKT1, AKT2, AKT3, MSK1, AMS2, PDK1, PLK3.
Price Range : 189.00 GBP - 516.00 GBP
SureFire principle

AlphaScreen SureFire Phospho-ATF2 (Thr71) Assay Kit

The AlphaScreen® SureFire® p-ATF2 (Thr71) assay is a sandwich immunoassay for quantitative detection of phospho-ATF2 (phosphorylated on Thr71) in cellular lysates using Alpha Technology.
Price Range : 936.00 GBP - 10120.00 GBP
PerkinElmer

LANCE Ultra Europium-anti-phospho-Crosstide (GSK-3α Ser21)

Mouse monoclonal antibody labeled with the LANCE® Eu-W1024-ITC chelate. This antibody recognizes specifically phosphorylated Ser21 in glycogen synthase kinase-3 alpha (GSK-3α), Crosstide and other peptides derived from GSK-3α. GSK-3α can be phosphorylated by a variety of kinases including AKT1, AKT2, AKT3, MSK1, AMS2, PDK1, PLK3. Sequence motif recognized: GRPRTSpSFAEG
Price Range : 1110.00 GBP - 3600.00 GBP
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra STAT1 pY701, 50,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-STAT1 (Tyr701) assay is a sandwich immunoassay for quantitative detection of phospho-STAT1 (phosphorylated on Tyr701) in cellular lysates using Alpha Technology.
List Price : 17820.00 GBP
PerkinElmer

LANCE Ultra Europium-anti-phospho-Myelin Basic Protein (MBP)

Mouse monoclonal antibody labeled with the LANCE® Eu-W1024-ITC chelate. This antibody recognizes specifically phosphorylated Thr232 in myelin basic protein (MBP; Swiss-Prot: P02686). MBP can be phosphorylated by a variety of kinases including Erk1/Erk2. Sequence motif recognized: FFKNIVTPRpTPPPSQGK
Price Range : 175.00 GBP - 3600.00 GBP
alphascreen surefire total

AlphaScreen SureFire GAPDH Assay Kit

The AlphaScreen® SureFire® total GAPDH assay is a sandwich immunoassay for quantitative detection of GAPDH in cellular lysates using Alpha Technology. This assay is intended to be used as a cellular normalization assay.
Price Range : 1030.00 GBP - 11100.00 GBP
SureFire principle

AlphaScreen SureFire Phospho-p53 (Ser392) Assay Kit

The AlphaScreen® SureFire® p-p53 (p-Ser392) assay is a sandwich immunoassay for quantitative detection of phospho-p53 (phosphorylated on Ser392) in cellular lysates using Alpha Technology.
Price Range : 936.00 GBP - 10120.00 GBP
PerkinElmer

ProfilerPro Kinase Peptide Substrate 10, for LabChip EZ Reader

Fluorescently-labelled peptide substrate 10 for kinase assays (inhibiton, target discovery, lead optimization)
List Price : 555.00 GBP
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-VEGF Receptor 2 (Tyr1175) Assay Kit

The AlphaLISA® SureFire® Ultrap-VEGF Receptor 2 (Tyr1175) assay is a sandwich immunoassay for quantitative detection of phospho-VEGFR Receptor 2 (phosphorylated on Tyr1175) in cellular lysates using Alpha Technology.
Price Range : 307.00 GBP - 17790.00 GBP
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra Total SLP-76 Assay Kit

The AlphaLISA® SureFire® Ultra Total SLP-76 assay is a sandwich immunoassay for quantitative detection of SLP-76 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
Price Range : 298.00 GBP - 17300.00 GBP
PerkinElmer

ProfilerPro Kinase Peptide Substrate 4, for LabChip EZ Reader

Fluorescently-labelled peptide substrate 4 for kinase assays (inhibiton, target discovery, lead optimization)
List Price : 555.00 GBP
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-SMAD3 (Ser423/425) Assay Kit

The AlphaLISA® SureFire® Ultra p-SMAD3 (Ser423/Ser425) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD3 (phosphorylated on Ser423/Ser425) in cellular lysates using Alpha Technology.
Price Range : 307.00 GBP - 17790.00 GBP

GTPγS, [35S]- 1250Ci/mmol, 12.5mCi/ml

Guanosine triphosphate, labeled on the gamma phosphate group with 35S (GTPγS).
Price Range : 690.00 GBP - 1210.00 GBP
SureFire principle

AlphaScreen SureFire EGFR (p-Tyr1068) Assay Kit

The AlphaScreen® SureFire® p-EGFR (Tyr1068) assay is a sandwich immunoassay for quantitative detection of phospho-EGFR (phosphorylated on Tyr1068) in cellular lysates using Alpha Technology.
Price Range : 1030.00 GBP - 11100.00 GBP

LANCE® Eu-W1024 Anti-phosphotyrosine (P-Tyr-100)

Europium-labeled anti-phosphotyrosine (P-Tyr-100) antibody, for use in LANCE® TR-FRET kinase assays.
Price Range : 126.00 GBP - 5820.00 GBP
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-MEK1 (Ser218/222) Assay Kit, 500 Assay Points

The AlphaLISA® SureFire® Ultra™ p-MEK1 (Ser218/222) assay is a sandwich immunoassay for quantitative detection of phospho-MEK1 (phosphorylated on Ser218/222) in cellular lysates using Alpha no-wash technology.
List Price : 1040.00 GBP
SureFire principle

AlphaScreen SureFire Phospho-GSK 3b (Ser9) Kit

The AlphaScreen® SureFire® p-GSK 3β (Ser9) assay is a sandwich immunoassay for quantitative detection of phospho-GSK3β (phosphorylated on Ser9) in cellular lysates using Alpha Technology.
Price Range : 936.00 GBP - 10110.00 GBP
PerkinElmer

ProfilerPro Kinase Peptide Substrate 18, for LabChip EZ Reader

Fluorescently-labelled peptide substrate 18 for kinase assays (inhibiton, target discovery, lead optimization)
List Price : 555.00 GBP
PerkinElmer

LANCE Ultra Europium-anti-phospho-MEK1/2 (Ser217/221) Antibody

Rabbit polyclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects human MEK1 phosphorylated at Ser217 and Ser221. It also reacts with MEK1 singly phosphorylated at Ser217 or Ser221. MEK1 is phosphorylated in vivo by RAF1 and COT. Homologous phosphorylation sites are found in MEK2. Sequence motif recognized: GQLIDSMANSFVG
Price Range : 1220.00 GBP - 4210.00 GBP
PerkinElmer

LANCE Eu-W1024 Anti-phosphotyrosine (PY20)

Europium-labeled anti-phosphotyrosine (PY20) antibody, for use in LANCE® TR-FRET kinase assays.
Price Range : 623.00 GBP - 5140.00 GBP

ATP, [γ-32P]- 10Ci/mmol 2mCi/ml

Adenosine triphosphate, labeled on the gamma phosphate group with 32P
Price Range : 120.00 GBP - 218.00 GBP
SureFire principle

AlphaScreen SureFire Phospho-AKT (Thr308) Kit

The AlphaScreen® SureFire® p-Akt1 (Thr308) assay is a sandwich immunoassay for quantitative detection of phospho-Akt1 (phosphorylated on Thr308) in cellular lysates using Alpha Technology.
Price Range : 936.00 GBP - 10110.00 GBP
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra STAT1 pY701 HV, 100 Assay Points

The AlphaLISA® SureFire® Ultra™ p-STAT1 (Tyr701) assay is a sandwich immunoassay for quantitative detection of phospho-STAT1 (phosphorylated on Tyr701) in cellular lysates using Alpha Technology.
List Price : 307.00 GBP

ATP, [γ-32P]- 3000Ci/mmol 5mCi/ml , 250 µCi

Adenosine triphosphate, labeled on the gamma phosphate group with 32P
List Price : 130.00 GBP
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-EGFR (Tyr1068) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-EGFR (Tyr1068) assay is a sandwich immunoassay for quantitative detection of phospho-EGF Receptor (phosphorylated on Tyr1068) in cellular lysates using Alpha Technology.
Price Range : 307.00 GBP - 17790.00 GBP
SureFire principle

AlphaScreen SureFire Akt 123 (p-Ser473) Assay Kit

The AlphaScreen® SureFire® p-Akt1/2/3 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-Akt1/2/3 (phosphorylated on Ser473) in cellular lysates using Alpha Technology.
Price Range : 281.00 GBP - 11100.00 GBP

LANCE Ultra ULight™-poly GT

Poly Glu-Tyr (4:1) labeled with ULightTM dye. This random polymer is a Tyrosine kinase substrate.
Price Range : 189.00 GBP - 516.00 GBP
SureFire principle

AlphaScreen SureFire EIF4E (p-Ser209) Assay Kit

The AlphaScreen® SureFire® p-eIF4E (Ser209) assay is a sandwich immunoassay for quantitative detection of phospho-eIF4E (phosphorylated on Ser209) in cellular lysates using Alpha Technology.
Price Range : 1030.00 GBP - 11100.00 GBP
Alpha SureFire Ultra Multiplex kits

Alpha SureFire® Ultra™ HV Multiplex p-Akt 1/2/3 (S473) (Eu) + Total Akt1 (Tb) Kit

The Alpha SureFire® Ultra™ HV Multiplex p-Akt 1/2/3 (Ser473) + Total Akt 1 assay kit is used to measure both the phosphorylation (Ser473) and total levels of endogenous Akt in cellular lysates. The kit measures phosphorylation on Ser473 of Akt 1/2/3, and total levels of Akt 1 only. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure Akt (Ser473) activation by either recombinant or endogenous receptors, and can be applied to primary cells.
Price Range : 526.00 GBP - 30550.00 GBP
SureFire principle

AlphaScreen SureFire p53 (p-Ser15) Assay Kit

The AlphaScreen® SureFire® p-p53 (p-Ser15) assay is a sandwich immunoassay for quantitative detection of phospho-p53 (phosphorylated on Ser15) in cellular lysates using Alpha Technology.
Price Range : 256.00 GBP - 10110.00 GBP
PerkinElmer

LANCE Ultra Europium-anti-phospho-PLK (Ser137) antibody

Affinity-purified rabbit polyclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from polo-like kinase (PLK) and phosphorylated at serine 137. PLK (Ser137) peptides can be phosphorylated by a variety of kinases including Aurora A, PAK2, MSK1, PKA and PKC. Sequence motif recognized: RRRSLLE
Price Range : 916.00 GBP - 4210.00 GBP
SureFire principle

AlphaScreen SureFire 4EBP1 (p-Thr37/Thr46) Assay Kit

The AlphaScreen® SureFire® p-4EBP1 (Thr37/46) assay is a sandwich immunoassay for quantitative detection of phospho-4EBP1 (phosphorylated on Thr37/46) in cellular lysates using Alpha Technology.
Price Range : 1030.00 GBP - 11100.00 GBP
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-JNK1/2/3 (Thr183/Tyr185) Assay Kit, 10,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-JNK1/2/3 (Thr183/Tyr185) assay is a sandwich immunoassay for quantitative detection of phospho-JNK1/2/3 (phosphorylated on Thr183/Tyr185) in cellular lysates using Alpha no-wash technology.
List Price : 5740.00 GBP
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-SLP-76 (Ser376) Assay Kit, 10,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-SLP-76 (Ser376) assay is a sandwich immunoassay for quantitative detection of phospho-SLP-76 (phosphorylated on Ser376) in cellular lysates using Alpha no-wash technology.
List Price : 5740.00 GBP
PerkinElmer

ProfilerPro Kinase Peptide Substrate 13, for LabChip EZ Reader

Fluorescently-labelled peptide substrate 13 for kinase assays (inhibiton, target discovery, lead optimization)
List Price : 555.00 GBP
PerkinElmer

ProfilerPro Kinase Peptide Substrate 5, for LabChip EZ Reader

Fluorescently-labelled peptide substrate 5 for kinase assays (inhibiton, target discovery, lead optimization)
List Price : 555.00 GBP
SureFire principle

AlphaScreen SureFire CHK1 (p-Ser345) Assay Kit

The AlphaScreen® SureFire® p-CHK1 (Ser345) assay is a sandwich immunoassay for quantitative detection of phospho-CHK1 (phosphorylated on Ser345 in cellular lysates using Alpha Technology.
Price Range : 936.00 GBP - 10110.00 GBP
SureFire principle

AlphaScreen SureFire Akt 1 (p-Ser473) Assay Kit

The AlphaScreen® SureFire® p-Akt1 (Ser473) assay is a sandwich immunoassay for quantitative detection of phospho-Akt1 (phosphorylated on Ser473) in cellular lysates using Alpha Technology.
Price Range : 936.00 GBP - 10110.00 GBP

LANCE Ultra ULight™-JAK-1 (Tyr1023) Peptide

ULight™-labeled synthetic peptide derived from amino acids 1015-1027 of human Janus kinase 1 (JAK-1). The ULight-JAK-1 peptide can be phosphorylated at Tyr1023 by JAK3 and several other tyrosine kinases. Peptide sequence : CAGAGAIETDKEYYTVKD
Price Range : 234.00 GBP - 717.00 GBP

"Kinase" 1-50 of 251 Products and Services

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