PerkinElmer

Protein Kinase Research

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Cancer, diabetes, and Alzheimer’s research can benefit from the study of phosphorylation and protein kinase activity. Because of their role in signal transduction, kinase activity detection and characterization is significant for drug discovery.

We offer a range of end-point and real-time kinetic protein kinase assays, technologies, and instruments that allow rapid kinase detection, screening, and mechanism-of-action studies.

Our offerings include:

  • Labeled substrates for kinetic assays and antibody-free screening
  • AlphaLISA® kits for highly-sensitive detection of cellular phosphorylation
  • LANCE® TR-FRET protein kinase assays for rapid assay selection
  • Fast and simple assays using pre-validated peptide and protein substrates derived from biologically-relevant targets
  • Multimode plate readers for maximal speed and throughput independent of technology platform

Regulation and inhibition of kinase activity can open new pathways for therapeutics, especially in conditions related to cell signaling and growth factors.

Whether you work with serine/threonine-specific, tyrosine-specific, histidine-specific, or mixed kinases, our kinase activity assays and technologies enable rapid assay selection and accelerate drug development.

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Radioisotope
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Target Species
  • Human (104)

"Protein Kinase Research" 1-50 of 273 Products and Services

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EnSight multimode plate-reader

EnSight Multimode Plate Reader

The EnSight multimode plate reader combines labeled & image-based cytometry technologies in a single benchtop platform. Imaging & online data analysis is fast: a 384-well plate can be imaged in less than 5 mins. The system’s modular design, combined with workflow-based Kaleido data acquisition & analysis software, gives a truly versatile plate reader that gets users productive quickly.

LANCE Ultra ULight™-DNA Topoisomerase 2-alpha (Thr1342) Peptide

Synthetic peptide containing residues surrounding Thr1342 of human DNA Topoisomerase 2-alpha; phosphorylation site: Thr1342. The ULight-DNA Topoisomerase 2-alpha (Thr1342) peptide can be phosphorylated by PLK3, CK2α and other kinases. Phosphorylation motif: DEKTDDE
Price Range : 241.00 GBP - 531.00 GBP
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-EGFR (Tyr1068) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-EGFR (Tyr1068) assay is a sandwich immunoassay for quantitative detection of phospho-EGF Receptor (phosphorylated on Tyr1068) in cellular lysates using Alpha Technology.
Price Range : 316.00 GBP - 18330.00 GBP
SureFire principle

AlphaScreen SureFire Phospho-Topo IIa (Ser1106) Assay Kit

The AlphaScreen® SureFire® p-Topoisomerase II-α (p-Ser1106) assay is a sandwich immunoassay for quantitative detection of phospho-Topoisomerase II-α (phosphorylated on Ser1106) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 965.00 GBP - 10430.00 GBP

LANCE Ultra ULight™-Acetyl-CoA Carboxylase (Ser79) Peptide

ULight™-labeled synthetic peptide derived from residues 73-85 of rat Acetyl-CoA Carboxylase in which Ser77 is mutated to Ala (also known as SAMS). The ULight-Acetyl-CoA Carboxylase peptide can be phosphorylated at Ser 79 by the AMPK?1 kinase. Peptide sequence : CHMRSAMSGLHLVKRR
SureFire principle

AlphaScreen SureFire p70 S6K (p-Thr421/Ser424) Assay Kit

The AlphaScreen® SureFire® p-p70 S6K (Thr421/Ser424) assay is a sandwich immunoassay for quantitative detection of phospho-p70 S6K (phosphorylated on Thr421/Ser424) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit
Price Range : 965.00 GBP - 10430.00 GBP
SureFire principle

AlphaScreen SureFire InsulinR (p-Tyr1150;Tyr1151) Assay Kit

The AlphaScreen® SureFire® p-Insulin Receptor (Tyr1150/1151) assay is a sandwich immunoassay for quantitative detection of phospho-Insulin Receptor (phosphorylated on Tyr1150/1151) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1070.00 GBP - 11430.00 GBP
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-SLP-76 (Ser376) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-SLP-76 (Ser376) in a multiplexed assay.
Price Range : 226.00 GBP - 13610.00 GBP
SureFire principle

AlphaScreen SureFire Phospho-ATF2 (Thr71) Assay Kit

The AlphaScreen® SureFire® p-ATF2 (Thr71) assay is a sandwich immunoassay for quantitative detection of phospho-ATF2 (phosphorylated on Thr71) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 965.00 GBP - 10430.00 GBP

LANCE Ultra ULight™-PLK (Ser137) peptide

ULight™-labeled synthetic peptide containing the amino acid residues surrounding Ser137 of polo-like kinase (PLK). The ULight-PLK peptide can be phosphorylated at Ser137 by a variety of kinases including Aurora A, PAK2, MSK1, PKA and PKC. Peptide core motif : RRRSLLE
Price Range : 142.00 GBP - 516.00 GBP

AlphaScreen PhosphoSensor Kit

Antibody-free assay for kinase activity via dectection of phosphorylated protein or peptide substrate.
Price Range : 248.00 GBP - 1860.00 GBP
SureFire principle

AlphaScreen SureFire ELK1 (p-Ser383) Assay Kit

The AlphaScreen® SureFire® p-ELK1 (Ser383) assay is a sandwich immunoassay for quantitative detection of phospho-ELK1 (phosphorylated on Ser383) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 965.00 GBP - 10430.00 GBP
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-STAT3 (Tyr705) Assay Kit

The AlphaLISA® SureFire® Ultra p-STAT3 (Tyr705) assay is a sandwich style immunoassay for quantitative detection of phospho-STAT3 (phosphorylated on Tyr705) in cellular lysates using Alpha Technology.
Price Range : 316.00 GBP - 18330.00 GBP
SureFire principle

AlphaScreen SureFire CREB (p-Ser133) Assay Kit

The AlphaScreen® SureFire® p-CREB (Ser133) assay is a sandwich immunoassay for quantitative detection of phospho-CREB (phosphorylated on Ser133) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1070.00 GBP - 11430.00 GBP
AlphaLISA SureFire Ultra kits

Phospho-SLP-76 (Ser376) AlphaLISA SureFire Ultra Assay Kit

The AlphaLISA® SureFire® Ultra™ p-SLP-76 (Ser376) HV (high volume) assay is a sandwich immunoassay for quantitative detection of phospho-SLP-76 (phosphorylated on Ser376) in cellular lysates using Alpha no-wash technology.
Price Range : 316.00 GBP - 18330.00 GBP
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-STAT6 (Tyr641) Assay Kit

The AlphaLISA® SureFire® Ultra p-STAT6 (Tyr641) assay is a sandwich immunoassay for quantitative detection of phospho-STAT6 (phosphorylated on Tyr641) in cellular lysates using Alpha Technology.
Price Range : 316.00 GBP - 18330.00 GBP
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-CREB (Ser133) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-CREB (Ser133) in a multiplexed assay.
Price Range : 226.00 GBP - 13610.00 GBP

AlphaScreen Phosphotyrosine (PT66) Assay Kit

AlphaScreen phosphoprotein kits are designed to detection phosphoproteins in biochemical or cellular kinase assays. This kit contains streptavidin Donor beads and AlphaScreen Acceptor beads conjugated with the antiphosphotyrosine PT66 antibody. This kit can be used to capture biotinylated phosphoproteins, biotinylated phosphorylated peptides, or biotinylated antibodies in complex with phosphorylated proteins.
Price Range : 147.00 GBP - 8900.00 GBP
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-eIF4E (Ser209) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-eIF4E (Ser209) in a multiplexed assay.
Price Range : 226.00 GBP - 13610.00 GBP
PerkinElmer

ProfilerPro Kinase Peptide Substrate 6, for LabChip EZ Reader

Fluorescently-labelled peptide substrate 6 for kinase assays (inhibiton, target discovery, lead optimization)
List Price : 555.00 GBP
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra p-4E-BP1 (Thr37/46) Assay Kit

The AlphaLISA® SureFire® Ultra™ p-4E-BP1 (Thr37/46) assay is a sandwich immunoassay for quantitative detection of phospho-4EBP1 (phosphorylated on Thr37/46) in cellular lysates using Alpha Technology.
Price Range : 316.00 GBP - 18330.00 GBP
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular detection assay

Total AKT1 LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra total AKT1 kits are designed for the detection of total AKT1 (phosphorylated and unphosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay can be used as a normalization assay for phospho-AKT1, and is compatible with both adherent and suspension cells.
Price Range : 892.00 GBP - 4000.00 GBP
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

Phospho-eIF2A (Ser51) LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra phospho-eIF2A (Ser51) kits are designed for the detection of eIF2A (phosphorylated at S51) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
Price Range : 892.00 GBP - 4000.00 GBP
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-IGF Receptor β (Tyr1135/1136) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-IGF Receptor β (Tyr1135/1136) in a multiplexed assay.
Price Range : 226.00 GBP - 13610.00 GBP

ATP, [γ-32P]- 6000Ci/mmol 10mCi/ml

Adenosine triphosphate, labeled on the gamma phosphate group with 32P
Price Range : 166.00 GBP - 353.00 GBP
AlphaLISA SureFire Ultra kits

Phospho-p38 MAPK (Thr180/Tyr182) AlphaLISA SureFire Ultra Detection Kit

The AlphaLISA® SureFire® Ultra p-p38 MAPK (Thr180/Tyr182) assay is a sandwich immunoassay for quantitative detection of phospho-p38 MAPK (phosphorylated on Tyr180/182) in cellular lysates using Alpha Technology.
Price Range : 316.00 GBP - 19060.00 GBP
SureFire principle

AlphaScreen SureFire p-Smad3 (Ser423/425) research kit

The AlphaScreen® SureFire® p-SMAD3 (p-Ser423/425) assay is a sandwich immunoassay for quantitative detection of phospho-SMAD3 (phosphorylated on Ser423/425) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1070.00 GBP - 11430.00 GBP
SureFire principle

AlphaScreen SureFire Phospho-AKT (Thr308) Kit

The AlphaScreen® SureFire® p-Akt1 (Thr308) assay is a sandwich immunoassay for quantitative detection of phospho-Akt1 (phosphorylated on Thr308) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 965.00 GBP - 10430.00 GBP
SureFire principle

AlphaScreen SureFire ALK (p-Tyr1586) Assay Kit

The AlphaScreen® SureFire® p-ALK (Tyr1586) assay is a sandwich immunoassay for quantitative detection of phospho-ALK (phosphorylated on Tyr1586) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 965.00 GBP - 10430.00 GBP
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-STAT6 (Tyr641) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-STAT6 (Tyr641) in a multiplexed assay.
Price Range : 226.00 GBP - 13610.00 GBP
Assay schematic for LANCE <em>Ultra</em> TR-FRET cellular phosphorylation detection assay

Phospho-SMAD3 (Ser423/425) LANCE Ultra TR-FRET Cellular Detection Kit

LANCE Ultra phospho-SMAD3 (Ser423/425) kits are designed for the detection of SMAD3 (phosphorylated at residue S423/S425) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.
Price Range : 892.00 GBP - 4000.00 GBP
AlphaLISA SureFire Ultra kits

AlphaLISA SureFire Ultra Total SYK Assay Kit

The AlphaLISA® SureFire® Ultra Total SYK assay is a sandwich immunoassay for quantitative detection of SYK (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
Price Range : 301.00 GBP - 17460.00 GBP

ATP, [γ-32P]- 6000Ci/mmol 10mCi/ml EasyTide Lead

Adenosine triphosphate, labeled on the gamma phosphate group with 32P.
Price Range : 174.00 GBP - 369.00 GBP
SureFire principle

AlphaScreen SureFire p70 S6K (p-Thr229) Assay Kit

The AlphaScreen® SureFire® p-p70 S6K (Thr229) assay is a sandwich immunoassay for quantitative detection of phospho-p70 S6K (phosphorylated on Thr229) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 965.00 GBP - 10430.00 GBP
PerkinElmer

LANCE Ultra Europium-anti-phospho-Crosstide (GSK-3α Ser21)

Mouse monoclonal antibody labeled with the LANCE® Eu-W1024-ITC chelate. This antibody recognizes specifically phosphorylated Ser21 in glycogen synthase kinase-3 alpha (GSK-3α), Crosstide and other peptides derived from GSK-3α. GSK-3α can be phosphorylated by a variety of kinases including AKT1, AKT2, AKT3, MSK1, AMS2, PDK1, PLK3. Sequence motif recognized: GRPRTSpSFAEG
Price Range : 1140.00 GBP - 3710.00 GBP
SureFire principle

AlphaScreen SureFire p-VEGF2 Receptor (Tyr1175) research kit

The AlphaScreen® SureFire® p-VEGFR2 Receptor (p-Tyr1175) assay is a sandwich immunoassay for quantitative detection of phospho-VEGF2 Receptor (phosphorylated on Tyr1175) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1070.00 GBP - 11430.00 GBP
AlphaLISA SureFire Ultra kits

Phospho-JNK1/2/3 (Thr183/Tyr185) AlphaLISA SureFire Ultra Assay Kit

The AlphaLISA® SureFire® Ultra™ p-JNK1/2/3 (Thr183/Tyr185) HV (high volume) assay is a sandwich immunoassay for quantitative detection of phospho-JNK1/2/3 (phosphorylated on Thr183/Tyr185) in cellular lysates using Alpha no-wash technology.
Price Range : 316.00 GBP - 18330.00 GBP
SureFire principle

AlphaScreen SureFire IKKb (p-Ser177/Ser181) Assay Kit

The AlphaScreen® SureFire® p-IKKα (Ser177/181) assay is a sandwich immunoassay for quantitative detection of phospho-IKKα (phosphorylated on Ser177/181) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 965.00 GBP - 10430.00 GBP
SureFire principle

AlphaScreen SureFire cJun (p-Ser73) Assay Kit

The AlphaScreen® SureFire® p-c-Jun (Ser73) assay is a sandwich immunoassay for quantitative detection of phospho-c-Jun (phosphorylated on Ser73) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 965.00 GBP - 10430.00 GBP
SureFire principle

AlphaScreen SureFire JNK 1-3 (p-Thr183;Tyr185) Assay Kit

The AlphaScreen® SureFire® p-JNK1/3 (Thr183/Tyr185) assay is a sandwich immunoassay for quantitative detection of phospho-JNK1/3 (phosphorylated on Thr183/Tyr185) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 965.00 GBP - 10430.00 GBP
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-p38 MAPK (Thr180/Tyr182) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-p38 MAPK (Thr180/Tyr182) in a multiplexed assay.
Price Range : 226.00 GBP - 13610.00 GBP
AlphaLISA SureFire Ultra kits

Phospho-mTOR (Ser2448) AlphaLISA SureFire Ultra Assay Kit

The AlphaLISA® SureFire® Ultra™ p-mTOR (Ser2448) assay is a sandwich immunoassay for quantitative detection of phospho-mTOR (phosphorylated on Ser2448) in cellular lysates using Alpha no-wash technology.
Price Range : 316.00 GBP - 18330.00 GBP
DELFIA; Eu-N1 Rabbit Anti-Mouse-IgG

DELFIA Eu-N1 Anti-Phosphtyrosine (P-Tyr-100)

Europium-labeled anti-phosphotyrosine (P-Tyr-100) antibody, for use in DELFIA® time-resolved fluorescence (TRF) kinase assays, phosphoprotein detection, and phosphoprotein binding assays.
Price Range : 149.00 GBP - 1990.00 GBP
PerkinElmer

ProfilerPro Kinase Peptide Substrate 7, for LabChip EZ Reader

Fluorescently-labelled peptide substrate 7 for kinase assays (inhibiton, target discovery, lead optimization)
List Price : 555.00 GBP
PerkinElmer

ProfilerPro Kinase Peptide Substrate 21, for LabChip EZ Reader

Fluorescently-labelled peptide substrate 21 for kinase assays (inhibiton, target discovery, lead optimization)
List Price : 555.00 GBP
SureFire principle

AlphaScreen SureFire S6RP (p-Ser235;Ser236) Assay Kit

The AlphaScreen® SureFire® p-Ribosomal Protein S6 (p-Ser235/236) assay is a sandwich immunoassay for quantitative detection of phospho-RP S6 (phosphorylated on Ser235/236) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately, in addition to the AlphaScreen SureFire kit.
Price Range : 965.00 GBP - 10430.00 GBP
SureFire principle

AlphaScreen SureFire STAT5 (p-Tyr694;Tyr699) Assay Kit 

The AlphaScreen® SureFire® p-STAT5 (p-Tyr694/699) assay is a sandwich immunoassay for quantitative detection of phospho-STAT5 (phosphorylated on Tyr694/699) in cellular lysates using Alpha Technology. Please note that an AlphaScreen Protein A kit, which contains the Alpha Beads necessary to run this assay, needs to be purchased separately in addition to the AlphaScreen SureFire kit.
Price Range : 1070.00 GBP - 11430.00 GBP

AlphaScreen Phosphotyrosine (PY20) Assay Kit

AlphaScreen phosphoprotein kits are designed to detection phosphoproteins in biochemical or cellular kinase assays. This kit contains streptavidin Donor beads and AlphaScreen Acceptor beads conjugated with the antiphosphotyrosine PY20 antibody. This kit can be used to capture biotinylated phosphoproteins, biotinylated phosphorylated peptides, or biotinylated antibodies in complex with phosphorylated proteins.
Price Range : 116.00 GBP - 8900.00 GBP
Assay principle of Alpha Terbium SureFire Ultra assay

Phospho-IKKα (Ser176/180) Terbium SureFire Ultra Detection Kit

The Alpha SureFire® Ultra™ Multiplex kits allow the rapid, sensitive, and quantitative detection of two phosphoprotein targets in each well of an assay plate. This kit can be used in combination with any AlphaLISA SureFire Ultra assay to detect phospho-IKKα (Ser176/180) in a multiplexed assay.
Price Range : 226.00 GBP - 13610.00 GBP
PerkinElmer

LANCE Ultra Europium-anti-phospho-eIF4E-binding protein 1 (Thr37/46)

Europium-labeled rabbit monoclonal antibody recognizing human eukaryotic translation initiation factor 4E-binding protein 1 phosphorylated at Thr37 or Thr46. Sequence motif recognized: ST(pT)PGGTLFST(pT)PG
(mono-phosphorylated peptides at either residue will also be successfully recognized)
Price Range : 1260.00 GBP - 4350.00 GBP

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