PerkinElmer
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AlphaLISA SureFire Ultra p-Ribosomal Protein S6 (Ser240/244) Assay Kit, 500 Assay Points

The AlphaLISA® SureFire® Ultra™ p-rpS6 (Ser240/244) assay is a sandwich immunoassay for quantitative detection of phospho-Ribosomal Protein S6 (phosphorylated on Ser240/244) in cellular lysates using Alpha no-wash technology.

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For research use only. Not for use in diagnostic procedures.

Part Number
Unit Size
List Price
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Quantity
ALSU-PS6R-A-HV
100 Assay Points
316.00 GBP
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ALSU-PS6R-A500
500 Assay Points
1070.00 GBP
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ALSU-PS6R-A10K
10,000 Assay Points
5890.00 GBP
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ALSU-PS6R-A50K
50,000 Assay Points
18330.00 GBP
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Detail Information

The AlphaLISA® SureFire® Ultra™ p-rpS6 (Ser240/244) assay is a sandwich immunoassay for quantitative detection of phospho-rpS6 (phosphorylated on Ser240/244) in cellular lysates using Alpha Technology.

In the AlphaLISA® SureFire® Ultra™ assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure™ tag. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.

AlphaLISA® SureFire® Ultra™ kits are compatible with:

  • Cell and tissue lysates
  • Antibody modulators
  • Biotherapeutic antibodies

Alpha SureFire® kits can be used for:

  • Cellular kinase assays
  • Receptor activation studies
  • Screening

Specifications

Assay Pathway PI3K/Akt/mTOR
Assay Target S6RP
Assay Target Class Phosphoprotein
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA SureFire Ultra
Second Messenger Release Protein phosphorylation
Shipping Condition Blue Ice
Target Species Human
Unit Size 500 Assay Points