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AlphaLISA SureFire Ultra p-Ribosomal Protein S6 (Ser240/244) Assay Kit, 50,000 Assay Points

The AlphaLISA® SureFire® Ultra™ p-rpS6 (Ser240/244) assay is a sandwich immunoassay for quantitative detection of phospho-Ribosomal Protein S6 (phosphorylated on Ser240/244) in cellular lysates using Alpha no-wash technology.

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Part Number List Price Your Price Quantity
ALSU-PS6R-A50K 17820.00 GBP

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Overview

The AlphaLISA® SureFire® Ultra™ p-rpS6 (Ser240/244) assay is a sandwich immunoassay for quantitative detection of phospho-rpS6 (phosphorylated on Ser240/244) in cellular lysates using Alpha Technology.

In the AlphaLISA® SureFire® Ultra™ assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure™ tag. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.

AlphaLISA® SureFire® Ultra™ kits are compatible with:

  • Cell and tissue lysates
  • Antibody modulators
  • Biotherapeutic antibodies

Alpha SureFire® kits can be used for:

  • Cellular kinase assays
  • Receptor activation studies
  • Screening

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Pathway PI3K/Akt/mTOR
Assay Target S6RP
Assay Target Class Phosphoprotein
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA SureFire Ultra
Second Messenger Release Protein phosphorylation
Shipping Condition Blue Ice
Unit Size 50,000 Assay Points

Resources, Events & More

Resource Type File Name File Format
Brochure Alpha product listing PDF 132 KB
Manual Protocol for AlphaLISA SureFire Ultra rpS6 (Ser240/244) Assay Kits PDF 743 KB
Flyer Alpha SureFire Technology PDF 720 KB
Event Society of Laboratory Automation & Screening (SLAS) Event