The AlphaScreen® SureFire® Monoclonal p-ERK1/2 (Thr202/Tyr204) assay is a sandwich immunoassay for quantitative detection of phospho-ERK1/2 (phosphorylated on Thr202/Tyr204) in cellular lysates using Alpha Technology. This assay performs similarly to the TGRES SureFire kit, but contains a monoclonal antibody for phospho-ERK detection.
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Alpha SureFire® kits can be used for:
In the AlphaScreen® SureFire® assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with Protein A to immobilize the other antibody. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Phosphoprotein|
|Experimental Type||In vitro|
|Product Brand Name||AlphaScreen SureFire|
|Shipping Condition||Blue Ice|
|Unit Size||10,000 Assay Points|
|Resource Type||File Name||File Format|
|Manual||Protocol for AlphaScreen® SureFire® ERK 1/2 (p-Thr202/Tyr204) Monoclonal Assay Kits||PDF 336 KB|
|Flyer||Alpha SureFire Technology||PDF 720 KB|
|Event||Society of Laboratory Automation & Screening (SLAS)||Event|