The AlphaScreen® SureFire® total ERK assay is a sandwich immunoassay for quantitative detection of ERK (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies. This assay performs similarly to the TGRTES SureFire kit, but contains a monoclonal antibody for ERK detection.
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Alpha SureFire® kits can be used for:
In the AlphaScreen® SureFire® assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with Protein A to immobilize the other antibody. In the presence of protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Protein|
|Experimental Type||In vitro|
|Product Brand Name||AlphaScreen SureFire|
|Shipping Condition||Blue Ice|
|Unit Size||500 Assay Points|
|Resource Type||File Name||File Format|
|Manual||Protocol for AlphaScreen® SureFire® ERK 1/2 Total Monoclonal Assay Kits||PDF 499 KB|
|Flyer||Alpha SureFire Technology||PDF 720 KB|
|Event||Society of Laboratory Automation & Screening (SLAS)||Event|