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AlphaLISA Anti-Anti-O-linked GlcNAc Acceptor Beads, 25 mg

AlphaLISA® Acceptor beads conjugated to an antibody against O-linked n-acetylglucosamine (O-linked GlcNAc). These beads can be used for AlphaLISA no-wash assays.

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For research use only. Not for use in diagnostic procedures.

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Unit Size
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AL144C
250 µg
874.00 GBP
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AL144M
5 mg
4550.00 GBP
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AL144R
25 mg
15910.00 GBP
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Detail Information

AlphaLISA® Acceptor beads designed to detect O-linked n-acetylglucosamine (O-linked GlcNAc) in a homogeneous AlphaLISA assay. Broad species cross-reactivity is expected based on sequence similarity. Source of antibody: monoclonal.

The anti-O-linked GlcNAc AlphaLISA Acceptor beads were used for the development and optimization of an O-GlcNAc hydrolase (OGA) assay using a biotinylated Tau-derived peptide O-GlcNAcylated at Ser400 as substrate. A technical notes describing the assay is available in our product literature.

Features:

  • No-wash assay
  • Easy-to-automate
  • Fast assay optimization

AlphaScreen® and AlphaLISA® are bead-based assay technologies used to study biomolecular interactions in a microplate format. The acronym "Alpha" stands for amplified luminescent proximity homogeneous assay. As the name implies, some of the key features of these technologies are that they are non-radioactive, homogeneous proximity assays. Binding of molecules captured on the beads leads to an energy transfer from one bead to the other, ultimately producing a luminescent/fluorescent signal. To understand how a signal is produced, one must begin with an understanding of the beads. AlphaScreen and AlphaLISA assays require two bead types: Donor beads and Acceptor beads. Each bead type contains a different proprietary mixture of chemicals, which are key elements of the AlphaScreen technology. Donor beads contain a photosensitizer, phthalocyanine, which converts ambient oxygen to an excited and reactive form of O2, singlet oxygen, upon illumination at 680 nm. Please note that singlet oxygen is not a radical; it is molecular oxygen with a single excited electron. Like other excited molecules, singlet oxygen has a limited lifetime prior to falling back to ground state. Within its 4 µsec half-life, singlet oxygen can diffuse approximately 200 nm in solution. If an Acceptor bead is within that proximity, energy is transferred from the singlet oxygen to thioxene derivatives within the Acceptor bead, subsequently culminating in light production at 520-620 nm (AlphaScreen) or at 615 nm (AlphaLISA). In the absence of an Acceptor bead, singlet oxygen falls to ground state and no signal is produced. This proximity-dependent chemical energy transfer is the basis for AlphaScreen's homogeneous nature.

Specifications

Antibody Conjugates Anti-O-GlcNAc
Automation Compatible Yes
Bead Type or Core Bead Type AlphaLISA Acceptor
Detection Method Alpha
Experimental Type In vitro
Molecular Modification Glycosidation
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Unit Size 25 mg
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Brochure

Technical Note

AlphaLISA Tau-Ser400 O-GlcNAc hydrolase (OGA) Assay

AlphaLISA technology is a powerful and versatile platform that offers highly sensitive, no-wash immunoassays using Alpha Donor and AlphaLISA Acceptor beads. In this technical note, we present,the optimization of an OGA signal-decrease assay using as substrate a biotinylated Tau-derived peptide O-GlcNAcylated at Ser400.

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