PerkinElmer
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IL-8 (bovine/ovine) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® immunoassay kit for bovine IL-8 enables the detection and quantitation of bovine IL8 (bIL8) in serum, buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). This kit is 100% cross-reactive with Ovine IL-8.

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For research use only. Not for use in diagnostic procedures.

Part Number
Unit Size
List Price
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Quantity
AL545HV
100 assay points
449.00 GBP
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AL545C
500 assay points
982.00 GBP
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AL545F
5,000 assay points
6490.00 GBP
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Detail Information

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Interleukin-8 (IL-8), also known as CXCL8, is a chemokine produced by monocytes/macrophages, epithelial cells, endothelial cells and neutrophils, in response to inflammatory stimuli. IL8 is a potent chemoattractant for neutrophils, basophils, and T-cells, and causes degranulation of neutrophil specific granules and azurophilic granules. In addition, IL8 also has a wide range of other proinflammatory effects. IL8 has been linked to various pathologies, including bovine and ovine pneumonic pasteurellosis, bovine salmonellosis, ovine chronic interstitial lung disease and others. This assay allows the quantification of IL8 in bovine samples and given 100% crossreactivity with ovine IL8, could also be used to detected sheep IL8.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Specifications

Assay Target IL8
Assay Target Class Cytokine
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Shipping Condition Blue Ice
Target Species Bovine
Therapeutic Area Inflammation
Unit Size 5,000 assay points