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Chloramphenicol Biotin-free AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® biotin-free immunoassay kit for chloramphenicol enables the quantitative determination of chloramphenicol in buffered solution, milk and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The kit utilizes a Digoxigenin (DIG) / Anti-DIG interaction as opposed to the traditional Streptavidin/Biotin interaction. This enables optimal performance when working with biotin-rich media (e.g. RPMI) or samples containing endogenous biotin.

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Part Number Unit Size List Price Your Price Quantity
AL393HV 100 assay points 268.00 GBP
AL393C 500 assay points 953.00 GBP
AL393F 5,000 assay points 6320.00 GBP
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Overview

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Chloramphenicol is a broad spectrum antibiotic derived from the bacterium, Streptomyces venezuelae, that can be used to treat infections such as meningitis, plague, cholera, and typhoid fever. Due to its severe side effects, blood levels during treatment are monitored closely. The use of chloramphenicol to treat food-producing animals is banned in the United States, Canada, EU, and Australia due to the high potential risk of severe side effects. Food and water sources are strictly monitored for residues as any supply that is contaminated with chloramphenicol producing bacteria can result in exposure to humans.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA biotin-free assay, a DIG-labeled anti-analyte antibody binds to the anti-DIG-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target Chloramphenicol
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Therapeutic Area Food Testing
Unit Size 5,000 assay points

Resources, Events & More

Resource Type File Name File Format
Brochure Alpha product listing PDF  124 KB
Application Note Avoiding Biotin Interference in AlphaLISA Assays PDF  1 MB
Technical Note Detection and Quantification of Food Contaminants Using AlphaLISA PDF  1 MB
Data Sheet AlphaLISA Chloramphenicol Biotin-Free Detection Kit PDF  521 KB
Event Society of Laboratory Automation & Screening (SLAS) Event