The AlphaLISA® immunoassay kit for Chloramphenicol enables the quantitative determination of Chloramphenicol in buffered solution, sample extracts, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
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Chloramphenicol is a broad spectrum antibiotic derived from the bacterium, Streptomyces venezuelae, that can be used to treat infections such as meningitis, plague, cholera, and typhoid fever. Due to its severe side effects, blood levels during treatment are monitored closely. The use of chloramphenicol to treat food-producing animals is banned in the United States, Canada, EU, and Australia due to the high potential risk of severe side effects. Food and water sources are strictly monitored for residues as any supply that is contaminated with chloramphenicol producing bacteria can result in exposure to humans.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Therapeutic Area||Food Testing|
|Unit Size||500 assay points|
|Resource Type||File Name||File Format|
|Data Sheet||AlphaLISA Chloramphenicol Detection Kit||PDF 522 KB|
|Event||Society of Laboratory Automation & Screening (SLAS)||Event|