AlphaLISA® no-wash immunoassay kit for detection of Chinese Hamster Ovary Host Cell Proteins (CHO HCP) in buffered solution or cell culture medium.
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|Part Number||Unit Size||List Price||Your Price||Quantity|
|AL301C||500 assay points||926.00 GBP|
|AL301F||5,000 assay points||6150.00 GBP|
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CHO (Chinese Hamster Ovary) cells are commonly utilized as an expression system for the production of human recombinant proteins at industrial scale. However, since very frequently these proteins are intended to be used as therapeuticals for humans, they must be subsequently purified to remove undesired contaminants that could elicit toxic or immunological reactions. Therefore, there is a current need to quantitate and trace these unwanted host cell proteins in protein preparations with excellent sensitivity and robustness, while maintaining high throughput capabilities.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target||CHO HCP|
|Assay Target Class||Protein|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||5,000 assay points|
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|Resource Type||File Name||File Format|
|Brochure||Alpha Technology Solutions||PDF 4 MB|
|Brochure||Alpha product listing||PDF 124 KB|
|Application Note||A Comparison of AlphaLISA and TR-FRET Homogeneous Immunoassays in Serum-Containing Samples||PDF 1 MB|
|Technical Note||HiBlock Buffer Technical Data Sheet||PDF 333 KB|
|Data Sheet||Manual AlphaLISA CHO HCP (broad reactivity) AL301||PDF 222 KB|
|Poster||All-In-One-Well AlphaLISA Assays for Direct Biomarker Quantification in Cell Cultures||PDF 288 KB|
|Poster||AlphaLISA Assays are Homogeneous Sensitive Immunoassays for Detection of Analytesin a Variety of Biological Matrices||PDF 273 KB|
|Poster||Development of New AlphaLISA No-wash Immunoassay Kits for Sensitive, Rapid and Efficient Quantification of Cytokines||PDF 279 KB|
|Poster||Efficient AlphaLISA immunoassays for the detection and characterization of monoclonal antibody biotherapeutics||PDF 525 KB|
|Poster||New AlphaLISA assays for the development of monoclonal antibody biotherapeutics||PDF 501 KB|
|Flyer||AlphaLISA - Frequently Asked Questions||PDF 102 KB|
|Event||Society of Laboratory Automation & Screening (SLAS)||Event|