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CXCL4/PF4 (Human) AlphaLISA Detection Kit, 5000 Assay Points

The AlphaLISA® immunoassay kit for CXCL4/PF4 enables the quantitative determination of CXCL4/PF4 in human serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows minumum cross-reactivity with mouse CXCL4/PF4 and human CXCL4L1/PF4V.

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Part Number Unit Size List Price Your Price Quantity
AL398HV 100 assay points 388.00 GBP
AL398C 500 assay points 1680.00 GBP
AL398F 5,000 assay points 11630.00 GBP
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Overview

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Chemokine ligand 4 (CXCL4), otherwise known as platelet factor 4 (PF4) is a small 8 kDa cytokine belonging to the CXC chemokine family. During platelet aggregation, CXCL4/PF4 is released from alpha-granules of activated platelets. The active protein is a tetramer ring of PF4 subunits which binds with high affinity to heparin. The function of CXCL4/PF4 is to promote blood clotting by neutralization of the anticoagulant effect of heparin molecules, by binding them on the endothelial surface of blood vessels. Mature human CXCL4/PF4 shares 96% amino acid sequence identity with the variant CXCL4L1/PF4V and 75% with mouse CXCL4/PF4. During heparin administration, some patients generate antibodies against the CXCL4/PF4/heparin complex, which activate platelets and can cause heparin-induced thrombocytopenia (HIT) and/or arterial thrombosis. The CXCL4/PF4 AlphaLISA detection kit allows for the detection of CXCL4/PF4 in human serum, plasma, and cell culture supernatants.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target CXCL4/PF4
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Inflammation
Unit Size 5,000 assay points

Resources, Events & More

Resource Type File Name File Format
Brochure Alpha product listing PDF  124 KB
Data Sheet AlphaLISA CXCL4/PF4 (Human) Detection Kit PDF  408 KB
Event Society of Laboratory Automation & Screening (SLAS) Event