The AlphaLISA® Human BDNF Biotin-Free Detection Kit is designed for the quantitative detection of human Brain Derived Neurotrophic Factor (BDNF) in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit is compatible with high-biotin culture media and other sample types that contain high levels biotin.
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|Part Number||Unit Size||List Price||Your Price||Quantity|
|AL362HV||100 assay points||296.00 GBP|
|AL362C||500 assay points||982.00 GBP|
|AL362F||5,000 assay points||6490.00 GBP|
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The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).
Brain Derived Neurotrophic Factor (BDNF) is a neurotrophin that acts within the central and peripheral nervous systems. BDNF is expressed in a pro-form as pro-BDNF then later cleaved by plasmin, furin, or pro-convertase enzymes to become the mature form of BDNF. Released BDNF has been observed to bind to both TrkB and LNGFR (p75) receptors. Acting on these receptors, BDNF helps to support the differentiation of new neurons and synapses, promote neuron growth, and generally promote the survival of existing neurons. BDNF has been observed to potentially play a role in several major diseases such as Alzheimer’s, Parkinson’s and Huntington’s disease. This kit has been designed for the detection and quantification of BDNF in serum, and cell culture supernatants.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA biotin-free assay, a DIG-labeled anti-analyte antibody binds to the anti-DIG-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target||Factor VIII|
|Assay Target Class||Protein|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||500 assay points|
|Resource Type||File Name||File Format|
|Brochure||Alpha product listing||PDF 128 KB|
|Application Note||Avoiding Biotin Interference in AlphaLISA Assays||PDF 1 MB|
|Event||Society of Laboratory Automation & Screening (SLAS)|