PerkinElmer
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IL-12/IL-23 (human) AlphaLISA Detection Kit, 100 Assay Points

The AlphaLISA® immunoassay kit for human IL-12 enables the detection and quantitation of human Interleukin 12 p40 (IL12 p40) in human serum or cell culture medium using a homogeneous AlphaLISA assay (no wash steps). It is important to note that this assay will also detect IL-23 p40.

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For research use only. Not for use in diagnostic procedures.

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Unit Size
List Price
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AL382HV
100 assay points
450.00 GBP
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AL382C
500 assay points
1690.00 GBP
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AL382F
5,000 assay points
12660.00 GBP
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Detail Information

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Interleukin 12 p40 (IL12 p40) (also known as IL-12B, natural killer cell stimulatory factor 2, or cytotoxic lymphocyte maturation factor 2, p40) is a subunit of interleukin 12. It also serves as a subunit of Interleukin 23. This cytokine is involved in atopic diseases, asthma, eczema, rhinitis, and autoimmune diseases. IL12 appears to play a major role in auto-immune disease, in the resistance to bacterial and parasitic infections, in antiviral responses, and in the promotion of antitumor immunity. Increased plasma levels of IL12 p40 have been reported in certain neurological disorders and auto-immune diseases. This kit has been designed for the detection of human IL12 p40 in serum and cell culture supernatants.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Specifications

Assay Target IL12, IL23
Assay Target Class Cytokine
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Inflammation
Unit Size 100 assay points