PerkinElmer
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IL-17F (human) AlphaLISA Detection Kit, 5000 Assay Points

The AlphaLISA® immunoassay kit for IL17F is designed for the quantitative determination of human IL17F in human serum, plasma, cell lysate, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows no cross-reactivity with human IL-17A and with mouse IL17F, bovine IL17F, and rat IL17F. Cross-reactivity with other species has not tested.

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Part Number Unit Size List Price Your Price Quantity
AL391HV 100 assay points 388.00 GBP
AL391C 500 assay points 1160.00 GBP
AL391F 5,000 assay points 11630.00 GBP
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Overview

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

IL17F, also known as human interleukin 17, is a pro-inflammatory cytokine produced by T-helper cells. This cytokine has been found to inhibit the angiogenesis and endothelial cells and induce endothelial cells to produce other cytokines. Levels of this cytokine play an important role in asthma and chronic inflammatory disease such as psoriasis, multiple sclerosis, and rheumatoid arthritis. The present kit is designed to detect IL17F in serum, plasma, cell culture supernatants, and cell lysates.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target IL17F
Assay Target Class Cytokine
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Inflammation
Unit Size 5,000 assay points

Resources, Events & More

Resource Type File Name File Format
Brochure Alpha product listing PDF  124 KB
Event Society of Laboratory Automation & Screening (SLAS)