PerkinElmer
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Nidogen-1 (human) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® immunoassay kit for human Nidogen-1 enables the detection and quantitation of Human Nidogen-1 (NID-1; also known as Entactin) in human serum, plasma, and, cell lysyate, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

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For research use only. Not for use in diagnostic procedures.

Part Number
Unit Size
List Price
Your Price
Quantity
AL367HV
100 assay points
288.00 GBP
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AL367C
500 assay points
954.00 GBP
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AL367F
5,000 assay points
6300.00 GBP
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Detail Information

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Nidogen-1 (NID-1), also known as Entactin, is a member of the Nidogen family of glycoproteins and is a component of the basement membrane. This protein is known to interact with other extracellular proteins such as perclean, collagen type IV, laminin, and fibronectin to play an essential role cell interactions and adhesions. Nidogen-1 has been identified as a potential biomarker for endometrial and prostate cancers. The present kit is designed to detect NID-1 in human serum, plasma, cell culture supernatants, cell lysate, and tissue homogenates.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Specifications

Assay Target Nidogen-1
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Target Species Human
Therapeutic Area Cancer
Unit Size 5,000 assay points