PerkinElmer
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IL-13 (porcine) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® immunoassay kit for IL13 is designed for the quantitative determination of IL13 in porcine serum, plasma, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps). The assay shows minimal cross-reactivity with human IL13.

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Part Number Unit Size List Price Your Price Quantity
AL551HV 100 Assay Points 437.00 GBP
AL551C 500 Assay Points 1290.00 GBP
AL551F 5000 Assay Points 6490.00 GBP
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Overview

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Interleukin 13 (IL13) is secreted by many cell types, especially T helper type 2 (Th2) cells. IL-13 is functionally similar to the IL4 and an important mediator of allergic airway-inflammatory diseases including airway hyperresponsiveness, goblet cell metaplasia and mucus hypersecretion. IL13 is known to play an important role in early pregnancy, vaccination, porcine respiratory syndrome. Levels of IL13 are associated with airway inflammtion in asthma and other airway diseases. This kit is designed to detect IL13 in serum, plasma, and cell culture supernatants.p>

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target IL13
Assay Target Class Cytokine
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Target Species Porcine
Therapeutic Area Inflammation
Unit Size 500 Assay Points

Resources, Events & More

Resource Type File Name File Format
Brochure Alpha product listing PDF  124 KB
Application Note Antigen-Stimulated PBMC Cytokine Release Measured by AlphaLISA Bovine Cytokine Kits PDF  3 MB
Data Sheet AlphaLISA IL13 (Porcine) Detection Kit PDF  333 KB
Event Society of Laboratory Automation & Screening (SLAS) Event