The AlphaLISA Mouse/Rat IL-17A Biotin-Free Detection Kit is designed for the quantitative detection of mouse or rat IL-17 (IL-17A) in serum, cell culture medium, and other samples types using a homogeneous (no wash steps, no separation steps) assay. The biotin-free kit uses anti-DIG (anti-Digoxin) Donor beads instead of streptavidin Donor beads, which makes the kit compatible with high-biotin culture media and other sample types that contain high levels biotin (including brain/liver tissue extracts, milk and eggs).
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Interleukin 17A (IL17A) is the founding member of a group of cytokines called the IL17 family. The other members are IL17B, C, D, E, and F. Mature mouse or rat IL17A is a glycosylated homodimer formed of two 133 amino acid subunits. IL17A is produced by a subset of T helper cells. It is a proinflammatory cytokine that enhances T cell priming and stimulates macrophages, fibroblasts, endothelial and epithelial cells to produce mediators of inflammation like IL1, IL6, and TNFα. It is also critical for neutrophil activation and migration, and induces IL8, a key chemokine for neutrophils. IL17 has been involved in the proinflammatory patterns associated with joint inflammation and rheumatoid arthritis in mouse and human models and may be a novel therapeutic target for the treatment of these diseases.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA biotin-free assay, a DIG-labeled anti-analyte antibody binds to the anti-DIG-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
|Assay Target Class||Cytokine|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||5,000 assay points|