PerkinElmer
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TSLP (mouse) AlphaLISA Detection Kit, 100 assay points

The AlphaLISA® immunoassay kit for mouse Thymic stromal lymphopoietin (TSLP) enables the quantitative determination of mouse TSLP in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).

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For research use only. Not for use in diagnostic procedures.

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Unit Size
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AL568HV
100 Assay Points
388.00 GBP
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AL568C
500 Assay Points
1160.00 GBP
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AL568F
5,000 Assay Points
9300.00 GBP
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Detail Information

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Thymic stromal lymphopoietin (TSLP) is a cytokine that plays a critical role in maturation of T cells through antigen presenting cells. TSLP is mainly produced by epithelial cells and its expression is regulated by many other cytokines such as IL1β and TNFα. TSLP expression is linked to many disease states including asthma, inflammatory arthritis, atopic dermatitis, eczema, eosinophilic esophagitis and other allergic states. Thus, TSLP becomes one of many important biomarkers for airway inflammatory diseases. This kit is designated to detect and quantify the levels of TSLP in cell culture media and other biological matrices.

Specifications

Assay Target TSLP
Assay Target Class Cytokine
Automation Compatible Yes
Detection Method Alpha
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Target Species Mouse
Therapeutic Area Inflammation
Unit Size 100 Assay Points