PerkinElmer
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AlphaScreen Mouse IgG Detection Kit, 10,000 assay points

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Anti-mouse IgG conjugated AlphaScreen Acceptor beads.

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For research use only. Not for use in diagnostic procedures.

Part Number
Unit Size
List Price
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6760606C
500 assay points
116.00 GBP
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6760606M
10,000 assay points
1860.00 GBP
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6760606R
50,000 assay points
8900.00 GBP
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Detail Information

This bead kit can be used for many applications, including:

  • Antibody detection assays

  • Protein detection assays

  • Protein-protein interaction assays

  • Protein expression

  • Binding studies

    In a typical AlphaScreen assay, one biomolecule of interested is biotinylated and associates with the Streptavidin-coated Donor beads. The other biomolecule is an anti-species antibody, and associates with the corresponding AlphaScreen anti-species Acceptor beads. If the two biomolecules bind to each other, the Donor and Acceptor beads are brought into proximity. Excitation of the Donor beads causes the release of singlet oxygen, which diffuses and triggers the emission of light from the Acceptor beads when in proximity. The amount of light is directly proportional to the degree of interaction. Competition formats are also possible, using the biotinylated FITC probe provided in the kit.  This kit contains enough reagents to run 10,000 wells in 384-well format, using a 25 µL reaction volume.

Specifications

Antibody Conjugates Streptavidin/Anti-mouse IgG
Automation Compatible Yes
Product Brand Name AlphaScreen
Quantity in a Package Amount 10000.0 Units
Shipping Condition Blue Ice
Unit Size 10,000 assay points