PerkinElmer
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Anti-V5 AlphaLISA Acceptor Beads, 25 mg

AlphaLISA® Acceptor beads conjugated to anti-V5 antibody. The mouse monoclonal antibody detects proteins tagged with the V5 14-amino-acid tag (the 9-amino-acid tag has also been successfully bound by the antibody). 

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For research use only. Not for use in diagnostic procedures.

Part Number
Unit Size
List Price
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Quantity
AL129C
250 µg
380.00 GBP
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AL129M
5 mg
3790.00 GBP
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AL129R
25 mg
15590.00 GBP
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Detail Information

These beads can be used to capture V5-tagged proteins and peptides, and can be used in conjunction with Alpha Donor beads to create AlphaLISA no-wash assays for:

  • Protein-protein interaction assays
  • Protein detection assays
  • Enzymatic assays

In a typical AlphaLISA assay, 25 mg of Acceptor beads is sufficient to run 25,000-50,000 wells using a 50 µL reaction volume.

 

Specifications

Antibody Conjugates Anti-V5
Automation Compatible Yes
Bead Type or Core Bead Type AlphaLISA Acceptor
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Unit Size 25 mg
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Brochure

Handle Large Biomolecular Interactions With Ease

The interactions and bindingof proteins are implicated in a large number of biological processes. The needfor an efficient, highly sensitive assay to study large protein interactions is increasingly important. Alpha Technology is a highly flexible, homogeneous, no-wash assay ideal for the measurement of protein interactions and complexes as large as 200 nm in size

PDF 798 KB

Guide

Alpha Protein-Protein Interaction Quick Start Guide

Alpha has been used to study a wide variety of interactions, including protein:protein, protein:peptide, protein:DNA, protein:RNA, protein:carbohydrate, protein:small molecule, receptor:ligand, and nuclear receptor:ligand interactions. Both cell-based and biochemical interactions have been monitored, and applications such as phage display, ELISA, and EMSA (electrophoretic mobility shift assay) have been adapted to Alpha.

PDF 380 KB
ELISA to AlphaLISA Immunoassay Conversion Guide

This guide presents the simple conversion of an ELISA or other immunoassay to an AlphaLISA® immunoassay.

PDF 1 MB
User's Guide To Alpha Assays Protein:Protein Interactions

AlphaScreen® and AlphaLISA® are bead-based assay technologies used to study biomolecular interactions in a microplate format. The acronym “Alpha” stands for Amplified Luminescent Proximity Homogeneous Assay. The assay does not require any washing steps. Binding of proteins or other binding partners captured on the beads leads to an energy transfer from one bead to the other, ultimately producing a luminescent signal.

PDF 3 MB