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LANCE Ultra Europium-anti-O-linked-GlcNAc Antibody, 10 µg

Europium-labeled monoclonal antibody recognizing O-linked n-acetylglucosamine (O-linked GlcNAc). For use in LANCE® Ultra post-translational modification assays.

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TRF0413-D
10 µg
2140.00 GBP
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100 µg
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Detail Information

The LANCE® Ultra Europium-anti-anti-O-linked-GlcNAc antibody was used for the development and optimization of a O-GlcNAc hydrolase (OGA) assay using a biotinylated Tau-derived peptide O-GlcNAcylated at Ser400 as substrate. A technical note describing
the assay is available in our product literature.

Formats:

  • 10 µg = 1,500 assay points (384-well assay format)
  • 100 µg = 15,000 assay points (384-well format)

Proven, cost-efficient LANCE Ultra reagents can be used to quantitate peptide modifications, detecting specific meythylation and acetylation states. No wash, homogenous LANCE Ultra reagents are HTS friendly - design your own epigenetics screening strategy for greatest efficiency.


Epigenetic enzymatic assays are optimized using a biotinylated histone H3-derived peptide as substrate. The modified peptide is captured by the Eu-labeled antibody (Ab) and ULight-Streptavidin (SA) which bring the Eu donor and ULight acceptor dye molecules into close proximity. Upon irradiation at 320 or 340 nm, the energy from the Eu donor is transferred to the ULight acceptor dye which, in turn, generates light at 665 nm. The intensity of the light emission is proportional to the level of biotinylated substrate modification.

Specifications

Antibody Conjugates Anti-O-linked-GlcNAc
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Experimental Type In vitro
Fluorophore Eu-W1024
Molecular Modification GlcNAc
Product Brand Name LANCE Ultra
Shipping Condition Dry Ice
Unit Size 10 µg
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Brochure

Technical Note

LANCE Ultra Tau-Ser400 O-GlcNAc hydrolase (OGA) Assay

This LANCE Ultra immunodetection assay measures the hydrolysis of an O-GlcNAc moiety,from a biotinylated Tau-Ser400-O-GlcNAc peptide.

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