PerkinElmer
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Fluorescein-12-ddATP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.

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NEL402001EA
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Detail Information

Fluorescein labeled dideoxyadenosine triphosphate is biologically active and can be incorporated with a variety of DNA polymerases including Taq, Klenow fragment, and Sequenase™. It terminates further incorporation and enables identification of the complementary base for applications like DNA sequencing and detection of point mutations via single base extension.

Specifications

Detection Method Fluorescence
Fluorophore Fluorescein
Nucleotide ddATP
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Data Sheet

Fluorescein-12-ddATP - Technical Data

Fluorescent 2’, 3’-dideoxynucleotide analogs are biologically active with a variety of DNA polymerases. Since dideoxynucleotides are chain terminators, they result in the analog being extended in a base specific manner onto the 3’-end of the DNA chain.

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