LANCE Ultra total EGFR kits are designed for the detection of EGFR (phosphorylated and non-phosphorylated) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay can be used as a normalizaton assay for phospho-EGFR, and is compatible with both adherent and suspension cells.
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For research use only. Not for use in diagnostic procedures.
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Please note control lysates are NOT included in kits. Control lysates are sold separately, catalog number TRF4015S.
LANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).
The epidermal growth factor receptor (EGFR) is a receptor tyrosine kinase for a number of extracellular ligands in the EGF family. Ligand binding triggers a number of events including dimerization and auto-phosphorylation. Several major signaling cascades are activated by EGFR.
|Assay Target Class||Protein|
|Detection Method||Time-Resolved Fluorescence (TRF), TR-FRET|
|Product Brand Name||LANCE Ultra|
|Shipping Condition||Blue Ice|
|Unit Size||500 Assay Points|