The LANCE® Ultra Human IgA Detection Kit is designed for detection and quantitation of human immunoglobulin A in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
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|Part Number||Unit Size||List Price||Your Price||Quantity|
|TRF1262C||500 Assay Points||514.00 GBP|
|TRF1262M||10,000 Assay Points||6860.00 GBP|
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The LANCE Ultra Human IgA Detection Kit is designed for detection and quantitation of human immunoglobulin A in buffered solution and cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.
LANCE and LANCE (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.
Immunoglobulin A (IgA) is a class of antibody mainly present in mucosal areas. IgA is essentially secreted in a dimeric form, which is maintained in place by the J-chain, a polypeptide of 15 kDa rich in cysteine residues. There are two isotypes of IgA: IgA1 is prevalent in the serum while IgA2 is prevalent in secretions. IgA is present in the gut, respiratory tract, urogenital tract, and in mucous secretions like saliva, tears, colostrum, vaginal fluid, and breast milk. IgA is relatively resistant to degradation and can remain active in extreme environments such as the digestive tract. IgA binds to pathogen, but is a poor activator of the complement. IgA deposition in the glomerulus is associated with IgA nephropathy.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Antibody|
|Detection Method||Time-Resolved Fluorescence (TRF), TR-FRET|
|Product Brand Name||LANCE Ultra|
|Unit Size||500 Assay Points|
|Resource Type||File Name||File Format|
|Brochure||LANCE product listing||PDF 82 KB|
|Application Note||Quantifying Bcl-2 Expression Levels in Human Cells with LANCE Ultra TR-FRET||PDF 3 MB|
|Manual||LANCE Ultra human IgA Detection Kit||PDF 519 KB|
|Flyer||LANCE Ultra Biomarker Detection Kits||PDF 585 KB|