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TSA Tetramethylrhodamine Tyramide Reagent Pack, Reagents for 100-300 Slides

TSA® tetramethylrhodamine (TMR) reagent pack, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. These reagents amplify by depositing extra biotin in the localized area of the probe or antibody.

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SAT702001EA
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Detail Information

Note: If you have never used TSA Amplification
Reagents before, we strongly suggest purchasing the full TSA kit,
which includes a set of quality controlled reagents that have been
optimized to work together.


TSA reagent packs are provided in larger sizes for economical scale-up of assay. These packs include labeled tyramide and amplification diluent. Blocking reagent and HRP must be purchased or provided separately.

These systems are designed for direct fluorescence detection in
ISH or IHC procedures.


  • Signal amplification for IHC, ISH and related applications

  • Direct fluorescence detection

  • Up to 1000-fold higher sensitivity

  • Conserve precious antibodies or probes

  • High resolution result

  • Adds 2 steps to existing protocol for rapid results

Specifications

Detection Method Fluorescence
Fluorophore TMR
Product Brand Name TSA
Shipping Condition Blue Ice
Unit Size 100-300 Slides
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Application Note

Signal Amplification Strategies for Rapid and Sensitive High Content Analysis

TSA reduces exposure timein an imaging assay for PKCa. Protein kinase C alpha (PKCa) is associated with a wide variety of cellular processes including proliferation, adhesion and motility.

PDF 776 KB

Brochure

TSA™ Signal Amplification for IHC, ICC, IF

The extraordinary sensitivity of the Tyramide Signal Amplification (TSA™) kits from PerkinElmer lets you see previously undetectable levels of protein and nucleic acid. The resolution is remarkable. And with multi-target detection, you can get more information from each experiment. It’s clear to see. TSA makes it easy to gain valuable insight from your immunohistochemistry (IHC) and immunocytochemistry (ICC) results.

PDF 3 MB
TSA™ Signal Amplification for ISH

Tyramide Signal Amplification (TSA™) provides remarkable sensitivity enhancement for your ISH experiments without the drawbacks associated with other methods. Signal amplification with TSA allows detection at levels as low as asingle copy and enables the use of shorter probes for more precise localization of targets. The TSA reaction occurs within 10 minutes, and labels are bonded covalently, ensuring outstanding resolution.

PDF 2 MB