PerkinElmer

TR-FRET Toolbox Reagents

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Design your own assay by choosing your preferred donor- and acceptor-labeled reagents. The versatility of LANCE® TR-FRET technology allows the assembly of donor and acceptor dye pairs for use in protein binding and other assays. This is assay design at its most flexible -- featuring the most common affinity biomolecules and europium chelate-labeling reagents.

LANCE toolbox reagents can be used to design a variety of assays, including kinase, protein-protein interaction, protein-nucleic acid interaction, protein-small molecule interaction, epigenetic, receptor-ligand binding, nuclear receptor, receptor dimerization, ubiquitination, and other post-translational modifications, protease, and other binding and enzymatic assays.

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Antibody Conjugates
  • Anti-6X His (3)
  • Anti-acetyl-lysine (1)
  • Anti-c-Myc (2)
  • Anti-C-tag (2)
  • Anti-FITC (2)
  • Anti-FLAG (3)
  • Anti-GST (3)
  • Anti-H3K27ac (1)
  • Anti-H3K27me2 (1)
  • Anti-H3K27me3 (1)
  • Anti-H3K36me2 (1)
  • Anti-H3K4 (1)
  • Anti-H3K4me1-2 (1)
  • Anti-H3K9/H3K27 (1)
  • Anti-H3K9ac (1)
  • Anti-H3K9me2 (1)
  • Anti-H4R3me (1)
  • Anti-HA (1)
  • Anti-human IgG (2)
  • Anti-methyl arginine (1)
  • Anti-mouse IgG (3)
  • Anti-O-linked-GlcNAc (1)
  • Anti-P-Ser 14-3-3 (1)
  • Anti-P-Tyr-100 (1)
  • Anti-p53K382ac (1)
  • Anti-phospho-4EBP1 (1)
  • Anti-phospho-Acetyl-CoA-Carboxylase (1)
  • Anti-phospho-Crosstide (1)
  • Anti-phospho-glycogen synthase (1)
  • Anti-phospho-Histone H3 (2)
  • Anti-phospho-I?Ba (1)
  • Anti-phospho-MEK1/2 (1)
  • Anti-phospho-p70S6K (1)
  • Anti-phospho-PKC (1)
  • Anti-phospho-PLK (1)
  • Anti-phospho-rpS6 (1)
  • Anti-phospho-threonine (1)
  • Anti-phospho-Topo IIa (1)
  • Anti-phospho-Tyrosine Hydroxylase (1)
  • Anti-rabbit IgG (3)
  • Anti-V5 (2)
  • Biotin (1)
  • Protein A (1)
  • Protein G (1)
  • PT66 (1)
  • PY20 (1)
  • Streptavidin (5)
Application
  • Enzymatic (6)
  • Epigenetics (14)
  • Kinase (42)
  • Protein Analysis & Detection (17)
  • Protein-protein interactions (21)
Automation Compatible
  • Yes (89)
Detection Method
  • Time-Resolved Fluorescence (TRF) (82)
  • TR-FRET (82)
Fluorophore
  • Eu-W1024 (49)
  • Eu-W8044 (1)
  • SureLight APC (6)
  • SureLight-APC (1)
  • ULight (32)
Molecular Modification
  • Acetylation (6)
  • GlcNAc (1)
  • Methylation (9)
  • Phosphorylation (41)
Product Brand Name
  • LANCE (20)
  • LANCE SureLight (7)
  • LANCE Ultra (64)

"TR-FRET Toolbox Reagents" 1-50 of 89 Products and Services

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LANCE Ultra ULight™-Acetyl-CoA Carboxylase (Ser79) Peptide

ULight™-labeled synthetic peptide derived from residues 73-85 of rat Acetyl-CoA Carboxylase in which Ser77 is mutated to Ala (also known as SAMS). The ULight-Acetyl-CoA Carboxylase peptide can be phosphorylated at Ser 79 by the AMPK?1 kinase. Peptide sequence : CHMRSAMSGLHLVKRR

LANCE Ultra ULight™-PLK (Ser137) peptide

ULight™-labeled synthetic peptide containing the amino acid residues surrounding Ser137 of polo-like kinase (PLK). The ULight-PLK peptide can be phosphorylated at Ser137 by a variety of kinases including Aurora A, PAK2, MSK1, PKA and PKC. Peptide core motif : RRRSLLE
Price Range : 142.00 GBP - 516.00 GBP
PerkinElmer

SureLight Allophycocyanin-anti-Mouse antibody, 1 mg, lyophilized

APC-labeled anti-mouse IgG antibody, for capturing mouse IgG antibodies in LANCE TR-FRET assays.
List Price : 245.00 GBP

LANCE Ultra Europium-anti-acetyl-Histone H3 Lysine 27 (H3K27ac) Antibody

Europium-labeled monoclonal antibody recognizing human Histone H3 acetylated at lysine 27 (H3K27ac). For use in LANCE® Ultra epigenetic writer and eraser assays.
Price Range : 2140.00 GBP - 5360.00 GBP

LANCE Ultra Europium-anti-phospho-PKC (Ala25Ser) peptide Antibody

Mouse monoclonal antibody labeled with the LANCE® Europium chelate. This antibody detects phospho-Ser in a synthetic peptide (RFARKGSLRQKNV) only after the peptide is phosphorylated by PKC or PKA kinases. The synthetic peptide is derived from amino acids 19-31 of human PKCα in which Ala25 is mutated to Ser. Sequence motif recognized: RFARKG-pS-LRQKNV
Price Range : 1260.00 GBP - 4340.00 GBP

LANCE Ultra ULight™-Protein A

Protein A labeled with ULight™ dye.
Price Range : 246.00 GBP - 531.00 GBP
PerkinElmer

SureLight Allophycocyanin-streptavidin (APC-SA), protein binding assay, Iyophilized

APC-labeled streptavidin, for use in LANCE® TR-FRET protein binding assays.
Price Range : 245.00 GBP - 6880.00 GBP

LANCE Ultra ULight™-Histone H3 (Thr3/Ser10) Peptide

ULight™-labeled synthetic peptide derived from residues 463-480 of human RAC-alpha serine/threonine-protein kinase (AKT1 or PKB). AKT/PKB can be phosphorylated at Ser473 by the mTOR/Rictor complex and MAPKAP kinase 2. Peptide sequence : CSERRPHFPQFSYSASGTAR
Price Range : 181.00 GBP - 738.00 GBP

LANCE Ultra Europium-anti-unmodified Histone H3 Lysine 4 (H3K4) Antibody

Europium-labeled monoclonal antibody recognizing human Histone
H3 that is unmodified at lysine 4 (H3K4). For use in LANCE® Ultra epigenetic writer and eraser assays.
Price Range : 2140.00 GBP - 5360.00 GBP

LANCE Ultra Europium-anti-phospho-MEK1/2 (Ser217/221) Antibody

Rabbit polyclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects human MEK1 phosphorylated at Ser217 and Ser221. It also reacts with MEK1 singly phosphorylated at Ser217 or Ser221. MEK1 is phosphorylated in vivo by RAF1 and COT. Homologous phosphorylation sites are found in MEK2. Sequence motif recognized: GQLIDSMANSFVG
Price Range : 1340.00 GBP - 4340.00 GBP

ULight anti-c-myc antibody

ULight labeled anti-c-myc antibody for LANCE® TR-FRET assays.
Price Range : 276.00 GBP - 6990.00 GBP

LANCE Ultra Europium-anti-phospho-PLK (Ser137) antibody

Affinity-purified rabbit polyclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from polo-like kinase (PLK) and phosphorylated at serine 137. PLK (Ser137) peptides can be phosphorylated by a variety of kinases including Aurora A, PAK2, MSK1, PKA and PKC. Sequence motif recognized: RRRSLLE
Price Range : 943.00 GBP - 4340.00 GBP

LANCE Ultra ULight™-JAK-1 (Tyr1023) Peptide

ULight™-labeled synthetic peptide derived from amino acids 1015-1027 of human Janus kinase 1 (JAK-1). The ULight-JAK-1 peptide can be phosphorylated at Tyr1023 by JAK3 and several other tyrosine kinases. Peptide sequence : CAGAGAIETDKEYYTVKD
Price Range : 241.00 GBP - 738.00 GBP

LANCE Ultra Europium-anti-phospho-CREB (Ser133)

Monoclonal antibody labeled with the LANCE® Eu-W1024-ITC chelate. This antibody recognizes specifically phosphorylated Ser133 in the cAMP response element binding (CREB) protein. CREB (Ser 133) can be phosphorylated by a variety of kinases including MSK1, PKA and PKC isoforms.
Price Range : 685.00 GBP - 2960.00 GBP

ULight anti-FITC

ULight™ labeled anti-FITC antibody for LANCE® TR-FRET assays. The anti-FITC antibody is a purified mouse IgG2a monoclonal antibody.
Price Range : 276.00 GBP - 6990.00 GBP

LANCE Ultra Europium-anti-phospho-p70 S6K (Thr389) Antibody

Mouse monoclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects human p70 S6K phosphorylated at Thr389. p70 S6K is phosphorylated in vivo by mTOR. p70 S6K peptides are also phosphorylated by several kinases including COT, HGK, IKKε, IRAK1, IRAK4, MAP4K2, MINK, MST1, MST2, NEK1, NEK2, NEK6, NEK7, PEK, PLK1 and TAOK2. Sequence motif recognized: LGFTYVAP
Price Range : 943.00 GBP - 4340.00 GBP

LANCE Ultra ULight™-eIF4E-binding protein 1 (Thr37/46) Peptide

Synthetic peptide containing residues surrounding Thr37 and Thr46 of human eukaryotic translation initiation factor 4E-binding protein 1; phosphorylation sites: Thr37 and Thr46. The ULight-eIF4E-binding protein 1 peptide can be phosphorylated at Thr37/46 by mTOR and many kinases. Phosphorylation motif: STTPGGTLFSTTPG
Price Range : 246.00 GBP - 531.00 GBP
PerkinElmer

Eu-anti-V5 (LANCE TR-FRET)

Europium-labeled anti-V5 antibody, for capturing V5-tagged proteins in LANCE® TR-FRET assays. This Europium-labeled reagent can be used to capture V5-tagged proteins and peptides, and can be used in conjunction with LANCE® ULight or SureLight APC reagents to create TR-FRET no-wash assays for protein-protein interaction, protein detection, and enzymatic studies.
Price Range : 200.00 GBP - 5590.00 GBP

LANCE Ultra Europium-anti-O-linked-GlcNAc Antibody

Europium-labeled monoclonal antibody recognizing O-linked n-acetylglucosamine (O-linked GlcNAc). For use in LANCE® Ultra post-translational modification assays.
Price Range : 2140.00 GBP - 5350.00 GBP

LANCE Ultra Europium-anti-methyl-Histone H3 Lysine 4 (H3K4me1-2)

Europium-labeled monoclonal antibody recognizing human H3 mono- and di-methylated at lysine 4 (H3K4me1-2). For use in LANCE® Ultra epigenetic writer and eraser assays.
Price Range : 2140.00 GBP - 5360.00 GBP

ULight anti-mouse IgG

ULight™ labeled anti-mouse IgG antibody for LANCE® TR-FRET assays. The anti-mouse IgG antibody is a purified goat polyclonal antibody.
Price Range : 276.00 GBP - 6990.00 GBP

LANCE Ultra Europium-anti-phospho-Myelin Basic Protein (MBP)

Mouse monoclonal antibody labeled with the LANCE® Eu-W1024-ITC chelate. This antibody recognizes specifically phosphorylated Thr232 in myelin basic protein (MBP; Swiss-Prot: P02686). MBP can be phosphorylated by a variety of kinases including Erk1/Erk2. Sequence motif recognized: FFKNIVTPRpTPPPSQGK
Price Range : 181.00 GBP - 3710.00 GBP
PerkinElmer

LANCE® Eu-W1024 Anti-phosphotyrosine (PT66)

Europium-labeled anti-phosphotyrosine (PT66) antibody, for use in LANCE® TR-FRET kinase assays.
Price Range : 642.00 GBP - 5620.00 GBP
PerkinElmer

LANCE Eu-W1024 Biotin

Europium-labeled biotin for use in LANCE® TR-FRET assays.
List Price : 611.00 GBP
PerkinElmer

LANCE Eu-W1024 anti-FITC

Eu-W1024 labeled anti-FITC antibodies for LANCE® TR-FRET assays. The anti-FITC antibody is a purified mouse IgG2a monoclonal antibody.
Price Range : 206.00 GBP - 5760.00 GBP

LANCE Ultra Europium-anti-phospho-Tyrosine Hydroxylase (Ser40) antibody

Affinity-purified rabbit polyclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects peptides derived from tyrosine hydroxylase and phosphorylated at serine 40. Tyrosine hydroxylase peptides can be phosphorylated by a variety of kinases including PAK2, PKC, CHK1, Aurora A and PKA. Sequence motif recognized: RRQSLIE
Price Range : 1260.00 GBP - 4340.00 GBP
PerkinElmer

LANCE Eu-W1024 Anti-phosphotyrosine (PY20)

Europium-labeled anti-phosphotyrosine (PY20) antibody, for use in LANCE® TR-FRET kinase assays.
Price Range : 642.00 GBP - 5300.00 GBP

LANCE Ultra ULight™-DNA Topoisomerase 2-alpha (Thr1342) Peptide

Synthetic peptide containing residues surrounding Thr1342 of human DNA Topoisomerase 2-alpha; phosphorylation site: Thr1342. The ULight-DNA Topoisomerase 2-alpha (Thr1342) peptide can be phosphorylated by PLK3, CK2α and other kinases. Phosphorylation motif: DEKTDDE
Price Range : 241.00 GBP - 531.00 GBP

LANCE Ultra Europium-anti-acetyl-Histone H4 Lysine (H4Kac pan) Antibody

Europium-labeled monoclonal antibody recognizing human Histone H4 (H4Kac pan). For use in LANCE® Ultra epigenetic writer and eraser assays.
Price Range : 2140.00 GBP - 5350.00 GBP
PerkinElmer

LANCE Ultra ULight™-Tyrosine Hydroxylase (Ser40) peptide

ULight™-labeled synthetic peptide containing the amino acid residues surrounding Ser40 of tyrosine hydoxylase. The ULight-tyrosine hydroxylase peptide can be phosphorylated at Ser40 by a variety of kinases including Aurora A, PAK2, MSK1, PKA and PKC. Peptide core motif : RRQSLIE
Price Range : 189.00 GBP - 516.00 GBP
PerkinElmer

LANCE Eu-W1024 Anti-c-Myc

Europium-labeled anti-c-myc antibody, for capturing c-myc-tagged proteins in LANCE® TR-FRET assays.
Price Range : 183.00 GBP - 8830.00 GBP

LANCE® Eu-W1024 labeled anti-GST antibody

Europium-labeled anti-GST antibody, for capturing GST-tagged proteins in LANCE® TR-FRET assays.
Price Range : 130.00 GBP - 6420.00 GBP

LANCE Ultra ULight™-anti-6xHis

Mouse monoclonal anti-polyHistidine tag antibody labeled with ULight™ dye.
Price Range : 277.00 GBP - 6870.00 GBP
PerkinElmer

LANCE Eu-W1024 Anti-6xHis

Europium-labeled anti-6X His antibody, for capturing His-tagged proteins in LANCE® TR-FRET assays.
Price Range : 145.00 GBP - 6870.00 GBP

LANCE Ultra ULight™-phospho-40S ribosomal protein S6 (pSer235/236) Peptide

Synthetic peptide containing residues surrounding Ser235 and Ser236 of human 40S ribosomal protein S6; this peptide is pre-phosphorylated on Ser235; phosphorylation site: Ser236. This peptide is phosphorylated at Ser236 efficiently by kinases from the RSK family. Phosphorylation motif: RRL(pS)SLRA
Price Range : 305.00 GBP - 541.00 GBP
PerkinElmer

LANCE Eu-W1024 Anti-HA

Europium-labeled anti-HA antibody, for capturing HA-tagged proteins and peptides in LANCE® TR-FRET assays.
Price Range : 642.00 GBP - 8580.00 GBP

LANCE Ultra ULight™-Crosstide

Crosstide peptide (sequence: CGSGSGRPRTSSFAEG) labeled with ULight™ dye. Synthetic peptide derived from glycogen synthase kinase-3 alpha (GSK-3α) (Phosphorylation site: Ser21). GSK-3α can be phosphorylated by a variety of kinases including AKT1, AKT2, AKT3, MSK1, AMS2, PDK1, PLK3.
Price Range : 195.00 GBP - 531.00 GBP

LANCE Eu-W8044 Streptavidin

Europium-labeled streptavidin, for capturing biotinylated biomolecules in LANCE TR-FRET assays. The W8044 chelate provides more stability than the W1024 chelate.
Price Range : 642.00 GBP - 5620.00 GBP

LANCE Ultra Europium-anti-phospho-40S Ribosomal Protein S6 (Ser235/236)

Europium-labeled rabbit monoclonal antibody recognizing human 40S ribosomal protein S6 phosphorylated at both Ser235 and Ser236. Sequence motif recognized: RRL(pS)(pS)LRA
Price Range : 440.00 GBP - 1520.00 GBP

ULight anti-FLAG

ULight™ labeled anti-FLAG antibodies for LANCE® TR-FRET assays. The anti-FLAG antibody is a purified mouse IgG2a monoclonal antibody (clone 29E4.G7.H11). The antibody is know to recognize C-terminal and N-terminal FLAG-tagged proteins.
Price Range : 281.00 GBP - 6860.00 GBP

LANCE Ultra Europium-anti-phospho-eIF4E-binding protein 1 (Thr37/46)

Europium-labeled rabbit monoclonal antibody recognizing human eukaryotic translation initiation factor 4E-binding protein 1 phosphorylated at Thr37 or Thr46. Sequence motif recognized: ST(pT)PGGTLFST(pT)PG
(mono-phosphorylated peptides at either residue will also be successfully recognized)
Price Range : 1260.00 GBP - 4350.00 GBP
PerkinElmer

SureLight Allophycocyanin-anti-FLAG antibody, 1 mg, lyophilized

APC-labeled anti-FLAG antibody, for capturing FLAG-tagged proteins and peptides in LANCE TR-FRET assays.
List Price : 351.00 GBP

LANCE Ultra ULight™-CREBtide

CREBtide peptide (sequence: CKRREILSRRPSYRK) labeled with ULight™ dye. Synthetic peptide derived from human cAMP Response Element Binding (CREB) Protein (phosphorylation site: Ser133). CREB (Ser 133) can be phosphorylated by a variety of kinases including MSK1, PKA and PKC isoforms.
Price Range : 185.00 GBP - 531.00 GBP

ULight Anti-C-tag

ULight™ labeled anti-C-tag antibodies for LANCE® TR-FRET assays. This mouse monoclonal IgG1 antibody recognizes N-terminal and C-terminal Protein C tagged fusion proteins. The anti-C-tag antibody can be used to capture C-tag proteins, and can be used in conjunction with LANCE Europium-labeled reagents to create LANCE TR-FRET assays.
Price Range : 281.00 GBP - 6860.00 GBP

LANCE Ultra Europium-anti-acetyl-Histone H3 Lysine 9 (H3K9ac)

Europium-labeled monoclonal antibody recognizing human Histone H3 acetylated at lysine 9 (H3K9ac). For use in LANCE® Ultra epigenetic writer and eraser assays.
Price Range : 2140.00 GBP - 5360.00 GBP
PerkinElmer

LANCE Eu-W1024 Anti-Mouse IgG

Europium-labeled anti-mouse IgG, for capturing antibodies in LANCE® TR-FRET assays.
Price Range : 642.00 GBP - 5620.00 GBP

LANCE Ultra ULight™-anti-GST

Goat polyclonal anti-GST tag antibody labeled with ULight™ dye.
Price Range : 290.00 GBP - 6870.00 GBP

LANCE Ultra Europium-anti-di-methyl-Histone H3 Lysine 36 (H3K36me2) Antibody

Europium-labeled monoclonal antibody recognizing human Histone H3 di-methylated at lysine 36 (H3K36me2). For use in LANCE® Ultra epigenetic writer and eraser assays.
Price Range : 2190.00 GBP - 5360.00 GBP
PerkinElmer
Price Range : 245.00 GBP - 11370.00 GBP

LANCE Ultra Europium-anti-phospho-Crosstide (GSK-3α Ser21)

Mouse monoclonal antibody labeled with the LANCE® Eu-W1024-ITC chelate. This antibody recognizes specifically phosphorylated Ser21 in glycogen synthase kinase-3 alpha (GSK-3α), Crosstide and other peptides derived from GSK-3α. GSK-3α can be phosphorylated by a variety of kinases including AKT1, AKT2, AKT3, MSK1, AMS2, PDK1, PLK3. Sequence motif recognized: GRPRTSpSFAEG
Price Range : 1140.00 GBP - 3710.00 GBP

"TR-FRET Toolbox Reagents" 1-50 of 89 Products and Services