The AlphaLISA® Human Granulocyte/Macrophage Colony-Stimulating Factor (GM-CSF) Detection Kit is designed for detection and quantitation of human GM-CSF in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay.
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For research use only. Not for use in diagnostic procedures.
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Colony-stimulating factors (CSFs) are proteins necessary for the survival, proliferation, and differentiation of hematopoietic progenitor cells. The human Granulocyte/Macrophage colony-stimulating factor (GM-CSF) is a ~23 kDa glycosylated protein (144-aa), encoded by the CSF2 gene. It has been originally purified from culture media conditioned by lung tissue from endotoxin injected mice, having the capacity to stimulate the formation of both granulocyte and macrophage colonies.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
|Assay Target Class||Protein|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||5,000 assay points|