PerkinElmer
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AlphaLISA unmodified Histone H3 Lysine 4 (H3K4) Cellular Detection Kit, 5,000 Assay Points

This kit is designed to be used as a control for the AlphaLISA Epigenetic Cellular Detection Kits, through detection of unmodified Histone H3 Lysine 4 (H3K4) in cell lysates using a homogeneous AlphaLISA assay (no wash steps).

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For research use only. Not for use in diagnostic procedures.

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Unit Size
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AL719HV
100 assay points
428.00 GBP
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AL719C
500 assay points
1330.00 GBP
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AL719F
5,000 assay points
7620.00 GBP
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Detail Information

The AlphaLISA® Epigenetic Cellular Detection Kits enable rapid and direct detection of endogenous modification of epigenetic marks on histone H3. These no wash, all-in-one-well assays are optimized for simplicity and throughput.

  • Simple assay protocol amenable to automation, eliminating tedious ELISAs and Westerns
  • Suitable for endogenous and recombinant cell lines, providing the flexibility to work with relevant cell models
  • Ideal for HTS protocols with demonstrated Z’ factors greater than 0.6

The AlphaLISA detection of epigenetic marks in cellular extracts is performed as follows: cell cultured in the presence of compounds are lysed with the Cell-Histone Lysis buffer. Histones are then extracted from the nucleosomes by the addition of the Cell-Histone Extraction buffer. AlphaLISA anti-mark Acceptor beads and Biotinylated anti-Histone H3 (C-terminus) antibodies are then added for the capture of histone proteins carrying the mark of interest. After incubation, Streptavidin Donor beads are added for the capture of the biotinylated antibody. In the presence of histone proteins bearing the mark of interest, the beads come into proximity. Excitation of the Donor beads provokes the release of singlet oxygen molecules that trigger a cascade of energy transfer reactions in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Specifications

Assay Target Histone H3
Assay Target Class Histone
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Molecular Modification Unmodified
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Therapeutic Area Epigenetics
Unit Size 5,000 assay points
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Brochure

Epigenetics Drug Discovery Solutions

Epigenetics, the study of changes in gene expression caused by mechanisms that do not,alter DNA sequence, is a rapidly growing field which presents a significant opportunity for,drug discovery. As the understanding of the role of epigenetic changes in various disease,states advances, the need for high quality reagents that can rapidly identify and validate,therapeutic molecules that target these enzymes is increasing.

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