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LANCE Ultra Europium-anti-di/mono-methyl-Histone H3 Lysine 27 (H3K27me2-1) Antibody, 100 µg

Europium-labeled monoclonal antibody recognizing human Histone H3 mono- and di-methylated at lysine 27 (H3K27me2-1). For use in LANCE® Ultra epigenetic writer and eraser assays.

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TRF0406-D
10 µg
2140.00 GBP
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TRF0406-M
100 µg
5360.00 GBP
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Detail Information

The LANCE® Ultra Europium-anti-di/mono-methyl-Histone H3 Lysine 27 (H3K27me2-1) antibody was used for the development and optimization of a EZH2 methyltransferase assay using a biotinylated Histone H3 (21-44) as substrate. A JMJD3 demethylase assay was also developed, using a biotinylated Histone H3 (21-44) peptide tri-methylated at lysine 27 as substrate. A technical note describing
the assay is available in our product literature.

Formats:

  • 10 µg = 1,500 assay points (384-well assay format)
  • 100 µg = 15,000 assay points (384-well format)

Proven, cost-efficient LANCE Ultra reagents can be used to quantitate peptide modifications, detecting specific meythylation and acetylation states. No wash, homogenous LANCE Ultra reagents are HTS friendly - design your own epigenetics screening strategy for greatest efficiency.


Epigenetic enzymatic assays are optimized using a biotinylated histone H3-derived peptide as substrate. The modified peptide is captured by the Eu-labeled antibody (Ab) and ULight-Streptavidin (SA) which bring the Eu donor and ULight acceptor dye molecules into close proximity. Upon irradiation at 320 or 340 nm, the energy from the Eu donor is transferred to the ULight acceptor dye which, in turn, generates light at 665 nm. The intensity of the light emission is proportional to the level of biotinylated substrate modification.

Specifications

Antibody Conjugates Anti-H3K27me2
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Experimental Type In vitro
Fluorophore Eu-W1024
Molecular Modification Methylation
Product Brand Name LANCE Ultra
Shipping Condition Dry Ice
Unit Size 100 µg
Resources, Events & More
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Brochure

Poster

Development of Homogeneous Non-radioactive Assays for Studying Histone H3 Methyltransferases and Demethylases

Anti-mark antibodies coupled to AlphaLISA Acceptor beads or labeled with LANCE Ultra europium chelate were used for the successful optimization of robust and, sensitive epigenetic assays using histone H3-derived peptides as substrates.

PDF 381 KB
Development of Homogeneous Proximity Assays for JMJD2A/2C Histone Demethylases

In eukaryotes, the covalent modification of histones has a crucial role in chromatin architecture and plays an important part in a plethora of cellular processes, from chromatinre modeling and transcriptional regulation, to DNA repair and cell cycle control.

PDF 289 KB
Development of a Non-Radioactive, No-Wash Biochemical Assay for High-Throughput Screening of Small Molecule Modulators of DNA MethyltransferasesNathalie

Covalen modification of DNA through methylation is catalyzed by specific DNA methyltransferases (DNMTs).

PDF 386 KB

Technical Note

LANCE Ultra EZH2 Histone H3-Lysine 27 N-methyltransferase Assay

In this technical note, we present the optimization of an epigenetic enzymatic assay using a biotinylated histone H3-derived peptide as substrate. The modified peptide is captured by the Eu-labeled antibody(Eu-Ab) and ULight-Streptavidin (SA) which bring the Eu donor and ULight acceptor dye molecules into close proximity.

PDF 591 KB
LANCE Ultra JMJD3 Histone H3-Lysine 27 Demethylase Assay

In this technical note, we present the optimization of an epigenetic enzymatic assay using a biotinylated histone H3-derived peptide as substrate. The modified peptide is captured by the Eu-labeled antibody(Eu-Ab) and ULight-Streptavidin (SA) which bring the Eu donor and ULight acceptor dye molecules into close proximity.

PDF 550 KB
Lance Ultra p300 Histone H3-Lysine Acetyltransferase Assay

In this technical note, we present the optimization of an epigenetic enzymatic assay using a biotinylated histone H3-derived peptide as substrate. The modified peptide is captured by the Eu-labeled antibody(Eu-Ab) and ULight-Streptavidin (SA) which bring the Eu donor and ULight acceptor dye molecules into close proximity.

PDF 699 KB