PerkinElmer
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LANCE Eu-W1024 Anti-6xHis, 10 µg

Europium-labeled anti-6X His antibody, for capturing His-tagged proteins in LANCE® TR-FRET assays.

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AD0205
10 µg
145.00 GBP
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AD0110
50 µg
688.00 GBP
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AD0111
1 mg
6870.00 GBP
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Detail Information

Eu-W1024 labeled anti-6xHis antibodies for LANCE® assays.The anti-6xHis antibody is a purified mouse IgG1 monoclonal antibody. It is raised against a polypeptide containing a 6X histidine tag. The antibody has been shown to detect polyhistidine tags localized at the amino- or carboxyl-terminus.

LANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC), etc.]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm (for ULight dye and APC).

Specifications

Antibody Conjugates Anti-6X His
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Experimental Type In vitro
Fluorophore Eu-W1024
Product Brand Name LANCE
Shipping Condition Dry Ice
Unit Size 10 µg