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Opera Phenix High-Content Screening System

The Opera Phenix High Content Screening System is the premier confocal solution for today's most demanding high content applications. Drawing on over a decade of experience with the industry-leading Opera® High Content Screening System, the Opera Phenix is designed for high-throughput, phenotypic screening and assays involving complex disease models, such as live cells, primary cells and microtissues.

Part Number HH14000000
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For research use only. Not for use in diagnostic procedures.

Detail Information

The Opera Phenix HCS system's innovative optical design lets you generate richer information through extremely sensitive confocal imaging and at higher throughput than ever through simultaneous acquisition – without the issue of crosstalk.

Speed and Sensitivity – No Compromise

Proprietary Synchrony Optics combine a microlens enhanced Nipkow spinning disk with dual view confocal optics to separate fluorescence excitation and emission during simultaneous acquisition minimizing spectral crosstalk – for more speed and more sensitivity.

  • Up to four large format sCMOS cameras with simultaneous acquisition in up to four channels, so you can generate images of exceptional quality - faster than ever
  • Custom-designed high NA water immersion objectives to capture more photons and provide high image resolution even in thick samples
  • Large field of view – 290% larger than the original Opera HCS system to acquire more cells in a single image for robust assay statistics
  • State-of-the-art sCMOS image sensors deliver low signal-to-background noise, a wide dynamic range and high resolution – ideal for sensitive and quantitative measurements at short exposure times
  • Spinning microlens disk with increased pinhole-to-pinhole distance to further reduce out-of-focus noise in thick samples such as microtissues
  • Spinning disk optics and precise synchronization of the excitation laser and camera to minimize phototoxicity and bleaching for live cell assays
  • Widefield or confocal imaging modes depending on your assay requirements
  • Fast laser-based autofocus to ensure sharp confocal images from every field
  • Modular design to adapt the system to your changing application needs – choose from single, dual or four camera configuration with four solid state lasers or a five laser system optimized for FRET applications

With the Opera Phenix HCS System you can generate high resolution images in up to four colors at ultra-high throughput.

It All Comes Together in Harmony – Acquisition to Analysis Made Easy

  • Intuitive workflow user-interface guides you through the entire process, from acquisition to analysis and evaluation
  • Templates for set-up of acquisition channels and parameters to get you productive right away
  • Ready-made solutions for common image analysis tasks so you can simply select and go
  • Image analysis building blocks that allow you to create, configure, and customize your own high content analysis applications
  • Advanced analysis features such as texture and STAR morphology analysis for detailed descriptions of cellular morphology and robust differentiation of cellular phenotypes
  • Data management for automatic storage of analysis results and metadata, such as assay layout, instrument, settings, plus user-defined keywords, and annotations

The Harmony software has an intuitive user-interface that guides you through the whole process from acquisition to analysis and evaluation.

Your Total Solution for High Content Screening

  • Get better results from your Opera Phenix system with our for high content screening, such as the CellCarrir Ultra 384-well microplates designed for optimal performance in high content imaging applications and our CellCarrier Spheroid ULA plates for imaging 3D models.
  • Improve throughput and productivity, and reduce variability and reagent costs by automating your Opera Phenix system, for example with the cell::explorer automated workstation
  • Export your results automatically into the Columus Image Data Storage and Analysis System, so you can access, reanalyze, store, and share image data from Opera Phenix and other HCS systems across your organization
  • NEW! Export your results to High Content Profiler powered by TIBCO Spotfire®, and perform screening data analysis and validation, QC analyses, calculate reliable normalization, perform multivariate hit stratification and drug response profiling

Publications:

  • Barrows, NJ; Campos, RK; Powell, ST; Prasanth, KR. 2016. A Screen of FDA-Approved Drugs for Inhibitors of Zika Virus Infection. Cell Host Microbe, doi:10.1016/j.chom.2016.07.004
  • Conos, SA; Lawlor, KE; Vaux, DL; Vince, JE. 2016. Cell death is not essential for caspase-1-mediated interleukin-1ß activation and secretion. Cell Death Differentiation, doi:10.1038/cdd.2016.69
  • Fraietta, I. and Gasparri, F. 2016. The development of high-content screening (HCS) technology and its importance to drug discovery. Expert Opinion on Drug Discovery, Vol.11, 5. DOI:10.1517/17460441.2016.1165203
  • Kleensang, A; Vantangoli, MM; Odwin-DaCosta, S. 2016. Genetic variability in a frozen batch of MCF-7 cells invisible in routine authentication affecting cell function. Scientific Reports 6, Article number: 28994, doi:10.1038/srep28994
  • Letzsch, S; Boettcher, K; Kelm, JM. and Messner, S. 2015. Quantifying Efflux Activity in 3D Liver Spheroids : New Confocal Imaging Instruments Allow Screening in Complex Human Liver Microtissues. Genetic Engineering Biotechnology News. 35(7): 14-15. doi:10.1089/gen.35.07.08.
  • Li, L; Zhou, Q; Voss, TC; Quick, KL and LaBarbera, DV. 2016. High-throughput imaging: Focusing in on drug discovery in 3D. Methods 96 (2016) 97–102. doi.10.1016/j.ymeth.2015.11.013
  • Li, M; Wang, X; Cao, L; Lin, Z; Wei, M; Fang, M and Li, S. 2016. Quantitative and epitope-specific antigenicity analysis of the human papillomavirus 6 capsid protein in aqueous solution or when adsorbed on particulate adjuvants. Vaccine 34(37) 4422-4428.
  • Phillips, SL; Soderblom, EJ; Bradrick, SS; Garcia-Blanco, MA. 2016. Identification of proteins bound to dengue viral RNA in vivo reveals new host proteins important for virus replication. mBio 7(1):e01865-15. doi: 10.1128/mBio.01865-15.
  • Whalley, HJ et al. 2015. Cdk1 phosphorylates the Rac activator Tiam1 to activate centrosomal Pak and promote mitotic spindle formation. Nat. Commun.6:7437 doi: 10.1038/ncomms8437

Introducing the New Operetta CLS High-Content Analysis System

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Specifications

21 CFR Part 11 Compatible No
Automation Compatible Yes
Detection Method Transmission, Fluorescence
Height 45.0 cm
Imaging Modality High Content
Interface Ethernet
Length 138.0 cm
Light Source Laser
Portable No
Product Brand Name Opera Phenix
Warranty Yes
Width 66.0 cm
Resources, Events & More
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Application Note

Analyzing ERK Signal Transduction in Live Cells Using a FRET-Based Biosensor

Extracellular signal-regulated kinase (ERK) is a key component in the regulation of embryogenesis, cell differentiation, cell proliferation, and cell death. The ERK signaling pathway is altered in various cancer types and is frequently investigated as a target for therapeutic intervention. This application note describes how a live cell FRET assay to study ERK signaling was performed on the Operetta CLS™ high-content analysis system. The optimized design of the FRET-based biosensor, the high-quality imaging of the Operetta CLS system and the easy-to-use image analysis tools of the Harmony® software contribute to the robustness of the high-content assay.

PDF 1 MB
Automated Single Cell Tracking using the Operetta System: Analyzing Chemokinesis of Cancer Cells

In this application note, we describe a high-content screening application for analyzing the migration of non-small cell lung cancer cells in a live cell assay. Using the Operetta® high-content imaging system and digital phase contrast imaging, we tracked migrating cancer cells using automated single cell tracking in the Harmony® high-content imaging and analysis software.

PDF 1 MB
Distinguishing Cell Types by Phenotypic Profiling of the Nucleus

The promise of high-content screening is the acceleration of discovery by extracting as much relevant information as possible from cells. Nevertheless, a large percentage of high-content screens analyze only a small number of image-based properties. As nearly all screening approaches require a nuclear counterstain to facilitate segmentation, phenotypic profiling of the nuclei can offer new and additional perspectives on assays at no extra cost. This study shows how a single nuclear stain can enable phenotypic profiling and how phenotypic profiles can be used to distinguish up to seven different cell types, without further staining or phenotypic markers. Such methods could be applied to other fluorescent labels, opening up new horizons for unbiased drug discovery and disease research.

PDF 1 MB
Imaging Bile Canaliculi in 3D Liver Microtissues using the Opera Phenix HCS System

Analyzing transport of biliary metabolites is essential to predict pharmacokinetics and hepatotoxicity during drug development. A functional impairment of hepatobilary transporters, such as bile salt export pump (BSEP) and multidrug resistance-associated protein 2 (MRP-2), is strongly associated with an increased risk of cholestatic liver injury. Here, we describe a 3D high-content screening assay to study hepatobiliary transporter function in InSphero human liver microtissues. Confocal imaging and automated image analysis were used to quantify BSEP and MRP-2-mediated efflux of fluorescent substrates into bile canaliculi.

PDF 2 MB
Measuring FRET using the Opera Phenix High-Content Screening System

Fundamental processes in living cells, such as apoptosis and signal transduction are controlled by proteins, often acting in concert with other protein partners through protein-protein interactions (PPIs). Inappropriate protein-protein recognition can fundamentally contribute to many diseases, including cancer. Therefore, inhibiting protein-protein interactions represents an emerging area in drug design.

PDF 1 MB
Phenotypic Analysis of Hypertrophy in Human iPSC-Derived Cardiomyocytes

Learn how a phenotypic screening assay to study time-dependent effects of endothelin-1-induced hypertrophy was set up using human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes. Learn how: The Opera Phenix system has been applied in the field of neurodegenerative diseases. In this assay, the Opera Phenix system is 4 times faster than the previous Opera® system. Primary neuron morphology is analyzed in a straightforward approach using Harmony software. Careful assay optimization can increase throughput, and minimize the data burden, without compromising assay performance.

PDF 1 MB
Phenotypic Profiling of Autophagy using the Opera Phenix High-Content Screening System

Cells constantly sense their environment and their response is a spatio-temporal summation of all signals. To maintain physiological stability, cells need to adjust to environmental changes, a process called homeostasis. One of the most important processes involved in maintaining homeostasis is autophagy, and its significance was recognized by the award of the Nobel Prize for Physiology in 2016 to Yoshinori Ohsumi for the discovery of its underlying mechanisms. Although this is not fully understood, it is believed that autophagy can prevent tumor development by degrading, for example, damaged organelles and protein aggregates.

PDF 4 MB

Brochure

Drug Discovery Screening Solutions Brochure

Info on PerkinElmer's complete solutions for high-throughput and phenotypic screening.

PDF 880 KB

Case Study

A Workflow to Characterize and Benchmark Human Induced Pluripotent Stem Cells

Human induced pluripotent stem cells (iPSCs) offer tremendous opportunities for disease modeling and discovery of novel therapeutics. The UK-based Human Induced Pluripotent Stem Cell Initiative (HipSci) aims to advance iPSC technology and pave the way to discovering how genomic variation impacts cellular phenotype by offering the scientific community access to a vast panel of cell lines with thorough characterization and data analysis tools. This case study details a phenotypic screen used to characterize human iPSCs on diverse extracellular matrix substrates, and a method for the capture of specific phenotypes emerging upon cell-to-cell contact.

PDF 1 MB
High-Content Analysis: Advancing Your Knowledge to Help Combat Infectious Disease

Infectious diseases remain a major burden to human health. The increased globalization of modern society that facilitates the spread of infectious diseases, and phenomena such as anti-microbial resistance, underscore the importance of the development of new preventative and therapeutic approaches. In our e-book, learn how high-content imaging and analysis plays a significant role in infectious disease research.

PDF 4 MB
Improving the Throughput of a Neuroprotection Assay Using the Opera Phenix High-Content Screening System

In this case study, we show how a previously described neuroprotection assay can be easily and directly transferred to the Opera Phenix™ high-content screening system, with a 4-fold decrease in acquisition time.

PDF 4 MB

Manual

Five Top Tips for a Successful High-Content Screening Assay with a 3D Cell Model System

Whether you’re familiar with high-content screening, or a newcomer, you’ll need the right tools and strategies to overcome the challenges of using complex 3D cell models in such an assay. For example, growing consistent, reproducible 3D cultures can be problematic and imaging large, thick cell samples can be challenging, while managing the huge volumes of data generated is perhaps the most demanding aspect of all. In this article, we provide our top tips for running a successful high-content screening assay using a 3D cell model. Learn how you can: Generate uniform 3D cell models, Get the best quality images, Minimize imaging time and volume of data, Get deeper insights from your 3D cell model and Avoid unnecessary data transfer steps.

PDF 1 MB

Technical Note

3D Volumetric Analysis of Luminal Spaces Inside Cysts or Organoids

High-content assays using 3D objects such as cysts or organoids can be challenging from the perspectives of both image acquisition and image analysis. In this technical note we describe how to image and analyze epithelial Madin-Darby canine kidney (MDCK) cysts in 3D on the Operetta CLS™ high-content analysis system.

PDF 1 MB
How to Perform Successful Long Term Live Cell Imaging in a High-Content Analysis System

Download our technical note to find out how you can overcome some of the challenges associated with long-term live cell imaging. Learn how you can perform successful five-day live cell imaging on Operetta CLS™ and Opera Phenix™ high-content systems, avoid phototoxicity with gentle digital phase contrast imaging, and analyze cell growth and morphology on a single cell level without fluorescence staining.

PDF 3 MB
Opera Phenix High-Content Screening System Technical Performance: Crosstalk Suppression

Simultaneous multi-color imaging is a commonly used approach to increase the speed of high-content screening systems. However, one of the main problems arising from this approach is spectral crosstalk which can limit assay sensitivity. To overcome this challenge, we have developed an innovative optical concept for the Opera Phenix™ High Content Screening System, known as Synchrony Optics™. The Opera Phenix system can be equipped with up to four large field of view (FOV) sCMOS cameras to maximize throughput and Synchrony Optics enable parallel acquisition of up to four spectrally adjacent channels at the same time with minimal crosstalk.

PDF 1 MB
Opera Phenix High-Content Screening System Technical Performance: Speed

The Opera Phenix™ high-content screening system’s state of the art hardware, combined with Harmony® imaging and analysis software, improves acquisition and analysis speed, enabling users to perform highly multi-parametric phenotypic screens effectively. In this technical note, learn how the Opera Phenix system’s Synchrony™ Optics and sCMOS cameras image up to 6x faster, how water immersion objectives combined with binning can reduce exposure time by approx. 20-fold and how image acquisition and analysis time can be reduced by >70% compared to a classical spinning disc confocal system.

PDF 708 KB
The Benefits of Automated Water Immersion Lenses for High-Content Screening

Balancing the key factors in HCS imaging - sensitivity, resolution and speed - can be challenging since they cannot be optimized independently: changing one impacts the others. Nevertheless, there is a way to overcome some of the obstacles and here we explain why the choice of the objective lens is critical.

PDF 1 MB