TSA® biotin reagent pack, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. These reagents amplify by depositing extra biotin in the localized area of the probe or antibody.
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Note: If you have never used TSA Amplification Reagents before, we strongly suggest purchasing the full TSA kit, which includes a set of quality controlled reagents that have been optimized to work together.
TSA reagent packs are provided in larger sizes for economical scale-up of assay. These packs include labeled tyramide and amplification diluent. Blocking reagent and HRP must be purchased or provided separately.
TSA Biotin Systems:
|Detection Method||Fluorescence, Chromogenic|
|Product Brand Name||TSA|
|Shipping Condition||Blue Ice|
|Unit Size||200-600 Slides|
TSA reduces exposure timein an imaging assay for PKCa. Protein kinase C alpha (PKCa) is associated with a wide variety of cellular processes including proliferation, adhesion and motility.
The extraordinary sensitivity of the Tyramide Signal Amplification (TSA™) kits from PerkinElmer lets you see previously undetectable levels of protein and nucleic acid. The resolution is remarkable. And with multi-target detection, you can get more information from each experiment. It’s clear to see. TSA makes it easy to gain valuable insight from your immunohistochemistry (IHC) and immunocytochemistry (ICC) results.
Tyramide Signal Amplification (TSA™) provides remarkable sensitivity enhancement for your ISH experiments without the drawbacks associated with other methods. Signal amplification with TSA allows detection at levels as low as asingle copy and enables the use of shorter probes for more precise localization of targets. The TSA reaction occurs within 10 minutes, and labels are bonded covalently, ensuring outstanding resolution.
The TSA Biotin System technology uses HRP to catalyze the deposition of the biotin-labeled tyramide (amplification reagent) onto tissue sections or cell preparation surfaces that have been previously blocked with proteins. The reaction is quick (less than 10 minutes) and results in the deposition of numerous biotin labels immediately adjacent to the immobilized HRP enzyme.